Defense responses of apple treated with the yeast Pichia guilliermondii and silicon against Penicillium expansum

Subject Areas : Plant Protection

Leila Farahani ¹ , Hassan Reza Etebarian ²

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Received: 2011-04-08 Accepted : 2011-10-11 Published : 2011-11-22

Keywords:

Abstract :

In this study variation of Peroxidase activity and phenolic compounds were studied after treating with Pichia guilliermondii (A6) and silicon (Si) and inoculation with Penicillium expansum (F1). The samples were taken in zero, second, fourth, sixth and eighth days after pathogen inoculation. The results showed that Peroxidase activity increased during these days and achieved the highest activity in sixth and eighth days. These results indicated that the phenolic compounds were in maximum amount in the second day after pathogen inoculation. These findings emphasized that increase in peroxidase activity and phenolic compounds could be the reason for induction of resistance in apple fruits.

References:

Benhamou, N. & Bélanger,R.R. 1998. Benzothiadiazole-mediated induced resistance to Fusarium oxysporum f. sp. radicis-lycopersici in tomato. Plant Physiology, 118:1203-1212.

Bélanger, R. R., Bowen, P. A., Ehret, D. L., & Menzies, J. G. 1995. Soluble silicon: Its role in crop and disease management of greenhouse crops. Plant Disease. 79:329-336.

Bi, Y., Tian, S.P., Guo, Y.R., Ge, Y.H. & Qin, G.Z. 2006. Sodium silicate reduces postharvest decay on Hami melons: Induced resistance and fungistatic effects. Plant Disease, 90:279-283.

Campos-Vargas, R. & Saltveit, M.E. 2002. Involvement of putative chemical wound signals in the induction of phenolic metabolism in wounded lettuce. Physiologia Plantarum, 114:73-84.

Chérif, M., Asselin, A., & Bélanger, R. R. 1994. Defense responses induced by soluble silicon in cucumber roots infected by Pythium spp. Phytopathology, 84, 236-242.

El-Ghaouth, A., Wilson, C. L., & Wisniewski, M. 1998. Ultrastructural and cytochemical aspects of the biological control of Botrytis cinerea by Candida saitoana in apple fruit. Phytopathology, 88: 282-291.

Epstein, E. 1999. Silicon. Annual Review of Plant Physiological Molecular Biology, 50: 641–64.

Etebarian, H. R. 1988. Evaluation of quantity changes in phenolic compound in races of barley during growth of Puccinia hordei, and their relation with the resistance of these races to leaf rust of barley. Iranian Journal of  plant pathology, 24: 61-67 (In Persian).

Etebarian, H.R., Sholberg, P.L., Eastwell, K.C., Sayler, R.J. 2005. Biological control of apple blue mold with Pseudomonas fluorescens. Canadian  Journal of Microbiology, 51: 591-598.

Ghasemi, R. 2010. Studies on Biological Control of  Blue and Grean Mold of Orange with Some Yesats and Antagonism Mechanisms Involved. MSc thesis. Aboureihan Campus, University of  Tehran. (In Persian).

Gholamnejad, J., Etebarian, H.R. & Sahebani, N. 2010. Biological control of apple blue mold with Candida membranifaciens and Rhodotorula mucilaginosa. African Journal of Food Science, 4: 001-007

Gong, Y., Toivonen, M.A., Lau, O.L. & Wiersma, A. 2001. Antioxidant system level in Braeburn apple is related to its browing disorder. Botanical Bulletin of Academic Sinica, 42:259-264.

Gorin, N., & Heidema, F.T. 1976. Proxidase activity in Golden Delicious apples as a possible parameter of ripening and senescence. Journal of Agricultural and Food Chemistry, 24: 200-201

Murphy, A.M., Holcombe, L.J. & Carr, J.P. 2000. Characteristics of salicylic acid-induced delay in disease caused by a necrotrophic fungal pathogen in tobacco. Physiological Molecular Plant Pathology, 57:47-54.

Qin, G.Z. & Tian, S.P. 2005. Enhancement of biocontrol activity of Cryptococcus laurentii by silicon and the possible mechanisms involved. Phytopathology, 95:69-75.

Stevens, C., Khan, V. A., Lu, J. Y., Wilson, C. L., Chalutz, E., Droby, S., Kabwe, M. K., Haung, Z., Adeyeye, O., Pusey, L. P., & Tang, A. Y. A. 1999. Induced resistance of sweet potato to Fusarium root rot by UV-C hormesis. Crop Protection, 18: 463-470.

Vetter, J.L., Steinoerg, M.P. Nelson, A. 1958. Quantitative determination of Proxidase in sweet corn. Journal of Agricultural and Food Chemistry, 6:39-41.