Identification of Pseudomonas syringae pv. syringae isolateds from stone fruit trees by specific fingerprinting with REP sequence

Subject Areas : Plant Protection

Saghar Ketabchi ¹ , Nader Hassanzadeh ²

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Received: 2008-11-12 Accepted : 2010-02-17 Published : 2009-12-22

Keywords:

Abstract :

Repetitive PCR fingerprinting of 24 strains of P. syringae pv. syringae isolated from different host/areas were compared with a standard strain of Pss from Greece (BPIC 242). Bacterial DNA was prepared with three known methods i.e. freeze-boil, whole colony boiling and directly from washed leaf surface. Initial result showed no purified genomic DNA extraction was necessary for rep-PCR analysis. On the other hand, Rep patherns of Pss showed some correlation between host specialization and its habitant with genomic diversity. Strains isolated from stone fruits formed a distinct cluster from wheat and sugar beet strains and a similar pathern was found among all stone fruits strains in confined geographical areas. Therefore it was found the rep-PCR technique as a rapid, simple and producible method to identify and classify these important plant pathogens.

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