Antibody production against Chlorpyrifos Pesticide (first experience in Iran)

Subject Areas : Plant Protection

Maryam Basirian ¹ , Sohrab Imani ² , Kambiz Larijani ³

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Received: 2011-08-25 Accepted : 2012-03-07 Published : 2011-01-21

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Abstract :

Nowadays use of pesticides is common in agriculture and so many  investigations are carried out annually for determination of pesticide residue in crops with gas and liquid chromatography. But these methods are expensive and time consuming. Developing new methods of  immunoassays like  ELISA is interesting for many researchers. In this research, an evaluation of production of antibody against chlropyrifos was done. Pesticide was broken, then conjucation was made with  protein BSA and a rabbit got immunized by injection. Then after different intervals the blood was collected and titer was determined  with ELISA  reader showing a great difference with the reference. This method is cheap, easy and more sensitive as compared to chromatography.  

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