Antagonistic Mechanisms of Trichoderma spp. against Rhizoctonia solani, the causal agent of chickpea wet root rot disease
Subject Areas : Plant Protection
Sediqqeh Mohammadi
1
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)
Bahram Mansoori
2
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Hamid Reza Zamani Zadeh
3
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Asghar Heydari
4
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)
Keywords:
Abstract :
Antagonistic mechanisms of three isolates of Trichodermaharzianum (THJ1, THJ2 & THB), one isolates of T.virens and one isolates of T.viride were studied against wet root rot agent of Iranian chickpea, Rhizoctoniasolani. In dual culture, all isolates of Trichoderma were able to affect the mycelial growth of R. solani. The isolate THJ2 had the highest percent of growth inhibition (72.77, 94.72 and 94.05 %) at three times interval. On the base of microscopic study R. solani was affected by Trichoderma through hyphal contact, hyphal penetration, hyphal coiling and hyphal fragmentation. The effect of Non Volatile Metabolites (NVM) of Trichoderma isolates on mycelial growth of R. solani indicated that the NVM of THJ2 inhibited the growth of pathogen more than other isolates at three times of measurement, using 5ml (62.34, 73.83 and 70.97 %) and 10 ml (68.04, 72.59 and 80.52 %). Volatile Metabolites (VM) of all Trichoderma isolates inhibited mycelial growth wherease T. harzianum (THB) had the highest effect at three times of measurement (68.02, 73.32 and 73.54 %).
Alexopoulos, C.J., Mims, C.W., & Blackwell, M. 1996. Introductory Mycology, 4th edition, John Wiley and sons Incorporated, New York.
Benitez, T., Rincon, A.M., Timon, M.C. & Cordon, A.C. 2004. Biocontrol mechanisms of Trichoderma strains. International Microbiology, 7(4): 249-260.
Burmeister, L. 2008. The antagonistic mechanisms employded by Trichodermaharzianum and their impact on the control of the bean rust fungus Uromycesappendiculatus. Ph.D. Thesis. University of Hannover, Germany.
Davet, P., Artgues, M. & Martin, C. 1981. Production en conditions non aseptiques d'inoculum de Trichoderma harzianum Rifai pour des essais de lutte biologique. Agronomie, 1(10):933-935 (In French with English abstract).
Deacon, J.W. 1991. Significance ecology in the development of biological agent against soilborn plant pathogens. Biocontrol Science and Technology, 1: 5-20.
De Marco, J.L., Valadares-Inglis, M.C. & Felix, C.R. 2003. Production of hydrolytic enzymes by Trichoderma sp. isolates with antagonistic activity against Crinipellis perniciosa, the causal agent of witches broom of cocoa. Brasilian Journal of Microbiology, 34(1): 33-38.
El- Katany, M.H., Gudelj, M., Robra, K.H., Elnaghy, M.A. & Gubitez, G.M. 2001. Characterization of Chitinase and endo ß-1,3 glucanase from Trichoderma harzianum Rifai T21 involved in control of the phytopathogen Sclerotium rolfsii. Applied Microbiology and Biotechnology, 562: 137-143.
Emma, W.G. & Simeon, O.K. 2008. The use of Trichoderma harzianum and Trichoderma viride as potential biocontrol agent against peanut microflora and their effectiveness in reducing aflatoxin contamination of infected kernels. Biothecnology, Asian Network for Scientific Information, 1-9.
Godtfredsen, W.O. & Vangedal, S. 1965. Trichodermin, a new sesquiterpen antibiotic. Acta Chimica Scandinavia, 19: 1088-1102.
Harman, G.E. 2000. Myths and dogmas of biocontrol, changes in perception drived from research on Trichoderma harzianum T-22. Plant Disease, 84: 377-393.
Lorito, M., Farkas, V., Rebuffat, S., Bodo, B. & Kubicek. C. 1996. Cell wall synthesis is a major target of mycoparasitic antagonism by Trichoderma harzianum. Journal of Bacteriology, 187:6382-6385.
Lumsden, R.D., Ridout, C.J., Vendemia, M.E. Harrison, D.J., Waters, R.M., & Walter, L.F. 1992. Characterization of major secondary metabolites produced in soilless mix by a formulated strain of the biocontrol fungus Gliocladium virens. Canadian Journal of Microbiology, 38: 1274-1280.
Mohammadi, S., Mansoori, B., Zamani Zade, H.R. & Heydari, A. 2004. Biological control of Rhizoctonia solani the casual agent of wet root rot of chickpea in greenhouse condition. Proceedings of the 16th Iranian Plant Protection Congress, Vol. 2, Plant Disease and Weeds, Tabriz University, Tabriz, Iran
Ogoshi, A. 1997. Ecology and pathogenecity of anastomosis and intera specific groups of Rhizoctonia. Annual Review of Phytopathology, 25:125-143.
Papavizaz, G.O. 1995. Trichoderma biology, ecology and potential for biocontrol. Annual Review of Phytopathology, 23: 28-54.
Zeppa, G., Allegron, G., Barbeni, M. & Guarda, P.A. 1991. Variability in the production of volatile metabolites by Trichoderma viride. Review of Plant Pathology, 70: 4735.
