Evaluation of Metabolic and Enzymatic Changes in Tears in Women During Menstrual Period

Subject Areas :

Vahab  Jafarian ¹ (Biology scienceZanjan University, Zanjan, Iran)
Zahra  Bayani ² (BranchZanjan azad eslamic University, Zanjan, Iran.)

Received: 2020-10-06 Accepted : 2019-12-22 Published : 2019-12-22

Keywords:

Abstract :

Inroduction & Objective: Human tears are a complex combination of proteins, enzymes, fats, metabolites, and electrolytes that in spite of keeping the eye surface clean and transparent, play an important role in nourishing the eye. Material and Method:The age of the women tested was ranging from 18 to 24 years old and had complete physical and visual health with similar diet during one month before sampling. Tear samples of women were prepared as treatment on the third day of menstruation and control samples three days after menstruation. Some tear proteins were also analyzed by FPLC and RP-HPLC. In the present study, some biochemical alterations such as changes in lysozyme, peroxidase, alpha-amylase, tyrosinase, and changes in metabolites such as glucose, phosphorus, and calcium were measured and compared in tears of women during menstruation and normal conditions. Results: Biochemical results showed that the concentration of proteins (quantitative and qualitative (SDS-PAGE)) and lysozyme, peroxidase and tyrosinase enzymes increased in menstrual women as compared to their normal state, while alpha-amylase, glucose, phosphorus, and calcium were lower in tears during menstruation. Conclusion: According to our study, the reduction of glucose, phosphorus and calcium in tears can be considered as an indicator in comparative studies and medical diagnosis in women under menstruation

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