The effect of Linden (Tilia platyphyllos) extract on memory retention in Alzheimer model adult male rats
Subject Areas :مژگان شبیری 1 , اکرم عیدی 2 , Pejman Mortazavi 3 , شهربانو عریان 4
1 - گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران،ایران
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران
3 - Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - دانشکده زیست شناسی، دانشگاه خوارزمی، تهران. ایران.
Keywords: Tilia platyphyllos, Memory, Streptozotocin, Rat,
Abstract :
Introduction: Trees from Tilia species are used around the world for their medicinal properties. In folk medicine, the linden (Tilia platyphyllos) flower is used as a diuretic, stomachic, antispasmodic, and sedative agent. This plant has different flavonoids. Flavonoids have significant antioxidant activity. In the present study, the effect of linden ethanolic extract on memory retention induced by intracerebroventricular (ICV) of streptozotocin (STZ) was investigated in adult male Wistar rats. Materials and methods: The rats were randomly divided into 9 groups: normal control, Sham-operated control (saline, ICV), linden extract (0.05, 0.1, and 0.2 g/kg intragastrically, daily) alone, Alzheimer control rats (STZ, 110 mg/ml, 3 l/site, ICV), linden extract (0.05, 0.1, and 0.2 g/kg intragastrically, daily) together with STZ, and treatment was performed accordingly. Animals were injected with STZ bilaterally, on the 1st and 3rd days. Administration of linden extract (0.05, 0.1 and 0.2 g/kg) was started 1 h before the first dose of STZ and continued up to 28 days. The learning and memory behavior was assessed by a passive avoidance test 28 days after the first dose and step through Latency (STL) and time in the dark compartment (TDC) were measured by shuttle box. Results: Our results showed that administration of linden extract (0.1 and 0.2 g/kg) significantly improved STZ-induced cognitive impairment. Linden extract decreased the levels of TDC, while increased STL levels. Conclusion: These results indicate that linden extract is effective in providing protection against memory deficit and neuronal damage induced by STZ.