Investigating Corona virus nucleocapsid protein (N) mutations in the domain effective in immunogenicity and its effect on serological diagnostic tests in samples isolated from infected patients in Zanjan
Corona virus nucleocapsid protein mutations effective in immunogenicity and its effect on serological diagnostic tests
Subject Areas : Molecular detection of biochemical and genetic markers
Samaneh Karimkhaniloui
1
,
Saeed Ghorbian
2
,
Sanaz Mahmazi
3
,
Changiz Ahmadizadeh
4
,
Keyvan Nedaee
5
1 - Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran
2 - Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran
3 - Department of Biology, Faculty of Basic Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
4 - , Departmenet of Microbiology, Ahar Branch, Islamic Azad University,Ahar, Iran.
5 - Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Keywords: Covid-19, mutation, nucleocapsid, IgG, IgM, protein N,
Abstract :
Background &Aim: Wellness requires the proper functioning of the immune system in all of the body systems. The immune system active by the cooperation of several immune sections. The immune cells and substances can identify and destroy foreign viral and microbial agents that worn out cells and cancer cells in the body. The aim of this study was to investigate the effect of high-speed sports activity on the anti-inflammatory factors lysozyme, LL-37 and HDB-2 in the saliva of obese adolescent boys. Materials &Methods: 32 male students voluntarily participated in the present study and were randomly assigned to four groups: 1) obese aerobic exercise, 2) normal weight aerobic exercise, 3) obese control without exercise, and 4) normal weight control without exercise. Anthropometric indices of height, weight and body mass index were measured. Before and after eight weeks of practice, Shatell-Run standard test was performed. Salivary samples of lysozyme, lactoferrin, lactate and C-reactive protein concentrations were taken after eight weeks of training with a frequency of three sessions per week with an intensity of 30 to 90% of maximum aerobic power. Using analysis of covariance, variables with a significance level of less than (p≥0.05) were included in the analysis. Results: The results showed that the salivary levels of lysozyme (p=0.001), LL-37 (p=0.002), and HDB2 (p=0.001) increased significantly, and the amount of increase in obese students was higher than in people with normal weight. Conclusion: The increased response of some salivary anti-inflammatory proteins following eight weeks of high-speed exercise training after increased activity may be due to the short-term responses of the immune system against the pressures caused by intense activity.
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