the effect of galbanic acid on the prevention of intra-abdominal adhesion after surgery in rat
Subject Areas :
Melika Montazeri
1
,
Firoozeh Saghaei
2
1 - Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Received: 2023-05-04
Accepted : 2023-06-21
Published : 2023-07-23
Keywords:
",
intra-abdominal adhesion",
galbanic acid",
rat",
Abstract :
Background and Aim: Abdominal adhesions are a common complication after abdominal surgery that can cause intestinal obstructions, pelvic pain, or infertility. This study was conducted with the aim of investigating the effect of galbanic acid on preventing the formation of intra-abdominal adhesions after surgery in rats.Material & Methods: 40 male Wistar rats were randomly divided into 4 groups of 10: after abdominal surgery and induction of adhesion, in the control group: 1cc of distilled water, in the galbanic acid group (GA5): galbanic acid with a dose of 5mg/kg, in the galbanic acid group (Ga10): galbanic acid with a dose of 10 mg/kg and in the tween group: 0.5 cc of tween (solvent of galbanic acid) was prescribed intraperitoneally for 10 days. After the end of the course, the rats were anesthetized, and the open abdomen and adhesive bands were observed and graded and analyzed with spss software and chi-square test. A tissue sample was removed from the adhesion site and after staining, it was observed under a microscope.Results: Galbanic acid in doses of 5 and 10 mg/kg significantly reduced adhesion compared to the control and tween groups. Histopathological studies show the beneficial effect of galbanic acid by reducing collagen fibers and fibrocytes.Conclusion: Galbanic acid with anti-fibrin, antioxidant and anti-inflammatory properties in doses of 5 and 10 mg/kg can prevent intra-abdominal adhesions in rats.
References:
1-Avsar FM, Sahin M, Aksoy F, Avsar AF, Aköz M, Hengirmen S, Bilici S. Effects of diphenhydramine HCl and methylprednisolone in the prevention of abdominal adhesions. The American journal of surgery. 2001 Jun 1;181(6):512-5.
BW H. Use of fibrinolytic agents in the prevention of postoperative adhesion formation. Fertil Steril. 2000; 74:203-12.
Emre A, Akin M, Isikgonul I, Yuksel O, Anadol AZ, Cifter C. Comparison of intraperitoneal honey and sodium hyaluronate-carboxymethylcellulose (SeprafilmTM) for the prevention of postoperative intra-abdominal adhesions. Clinics. 2009; 64:363-8.
Ward BC, Panitch A. Abdominal adhesions: current and novel therapies. Journal of Surgical Research. 2011 Jan 1;165(1):91-111.
Giusto G, Vercelli C, Iussich S, Audisio A, Morello E, Odore R, Gandini M. A pectin-honey hydrogel prevents postoperative intraperitoneal adhesions in a rat model. BMC veterinary research. 2016 Dec; 13:1-5.
Najafian A, Alavi A, Amjadigolpayegani K, Dadipour S, Safarimoradabadi A, Fallahi S, Faramarzi A. Post surgical adhesion prevention: Vitamin C or Satureja Khuzestanica. Veterinary Clinical Pathology The Quarterly Scientific Journal. 2013 May 22;7(1 (25) Spring):1786-92.
Zhang Q, Qiao H, Wu D, Lu H, Liu L, Sang X, Li D, Zhou Y. Curcumin potentiates the galbanic acid-induced anti-tumor effect in non-small cell lung cancer cells through inhibiting Akt/mTOR signaling pathway. Life sciences. 2019 Dec 15; 239:117044.
Chahardoli A, Mavaei M, Shokoohinia Y, Fattahi A. Galbanic acid, a sesquiterpene coumarin as a novel candidate for the biosynthesis of silver nanoparticles: In vitro hemocompatibility, antiproliferative, antibacterial, antioxidant, and anti-inflammatory properties. Advanced Powder Technology. 2023 Jan 1;34(1):103928.
Fatehi Hassanabad A, Zarzycki AN, Jeon K, Deniset JF, Fedak PW. Post-operative adhesions: a comprehensive review of mechanisms. Biomedicines. 2021 Jul 22;9(8):867.
Muir, W.W., Hubbell, J.A.E., Skarda, R.T. and Bednarski, M. Hand book of veterinary anesthesia. 2nd Ed., The Ohio State University College of Veterinary Medicine, Columbus, OH. Mosby,1995, p. 110.
Wei G, Wu Y, Gao Q, Shen C, Chen Z, Wang K, Yu J, Li X, Sun X. Gallic acid attenuates postoperative intra-abdominal adhesion by inhibiting inflammatory reaction in a rat model. Medical science monitor: international medical journal of experimental and clinical research. 2018; 24:827.
12. Abbasian B, Kazemini H, Esmaeili A, Adibi S. Effect of bovine amniotic fluid on intra-abdominal adhesion in diabetic male rats. Journal of Diabetes and its Complications. 2011 Jan 1;25(1):39-43.
Diamond MP, Burns EL, Accomando B, Mian S, Holmdahl L. Seprafilm® adhesion barrier:(2) a review of the clinical literature on intraabdominal use. Gynecological surgery. 2012 Sep;9(3):247-57.
Charboneau AJ, Delaney JP, Beilman G. Fucoidans inhibit the formation of post-operative abdominal adhesions in a rat model. PLoS One. 2018 Nov 21;13(11): e0207797.
Asadi S.Y, Parsai P, Karimi M, Rafiyan Kopaei M. The effect of ethanolic extract of green tea (Camellia Sieneses) on intra-abdominal adhesion following laparotomy in rats. Scientific Journal of Zanjan University of Medical Sciences (Journal of Advances in Medical and Biomedical Research). 2013;21(86):86-96.
16.Iranshahy M, Iranshahi M. Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gum-resin)—A review. Journal of ethnopharmacology. 2011 Mar 8;134(1):1-0.
Niazmand R, Razavizadeh BM. Ferula asafoetida: chemical composition, thermal behavior, antioxidant and antimicrobial activities of leaf and gum hydroalcoholic extracts. Journal of Food Science and Technology. 2021 Jun;58(6):2148-59.
Goudarzi S, Rassouli FB, Kahrizi D, Shirkhani P, Mahdifar M, Iranshahi M, Rafatpanah H, Keramati MR, Ayatollahi H. Comparing toxicity of galbanic acid, auraptene and umbelliprenin on adult T-cell leukaemia-lymphoma in normoxia and hypoxia. Cellular and Molecular Biology. 2022 Dec 31;68(12):17-20.
Sirizi MA, Ghalenoei JA, Allahtavakoli M, Forouzanfar H, Bagheri SM. Anticancer potential of Ferula assa-foetida and its constituents, a powerful plant for cancer therapy. World Journal of Biological Chemistry. 2023 Mar 27;14(2):28-39.
Kim KH, Lee HJ, Jeong SJ, Lee HJ, Lee EO, Kim HS, Zhang Y, Ryu SY, Lee MH, Lü J, Kim SH. Galbanic acid isolated from Ferula assafoetida exerts in vivo anti-tumor activity in association with anti-angiogenesis and anti-proliferation. Pharmaceutical research. 2011 Mar; 28:597-609.
Kohno S, Murata T, Sugiura A, Ito C, Iranshahi M, Hikita K, Kaneda N. Methyl galbanate, a novel inhibitor of nitric oxide production in mouse macrophage RAW264. 7 cells. Journal of natural medicines. 2011 Apr; 65:353-9.
Asadi SY, Parsaei P, Karimi M, Rafieian-kopaei M. Effect of ethanolic extract of green tea (Camellia sinensis) on intraabdominal adhesions in rats. J Zanjan Univ Med Sci. 2013;21(86):86-96.
Nazem Zumardi P, Sattari K. Macroscopic and microscopic examination of the anti-adhesion properties of honey and corticosteroids following experimental surgery in the abdominal cavity of rabbits. Clinical studies of large animals. 2022;9(2):24-35.
24. Davari H, Goharian V, Eghbal A. Experimental study of pentoxifylline in prevention of post-operative intraperitoneal adhesion band. Iranian Journal of Surgery. 2005;13(2)
Mostajeran F, Manouchehri M. Effect of Amniotic Fluid in Treatment of Post-Laparatomy Adhesion Formation in Rat Model. Journal of Isfahan Medical School. 2011;28(121):1668-75.
Pourjafar M, KARIMI I, Farid M, Maghsoudi E, Shakhs NM. The comparison of standard heparin and dalteparin on prevention of adhesion in peritonitis with or without infection in rat. Journal of Veterinary Research. 2007; 62(50:277-280,2007
Panahi F, SHAHRAM E, Khoshmohabat H, Kaka GR, Sadraei S, Hossein AM. Macroscopic and microscopic assessment of normal saline on postoperative adhesion formation in a rat model. Iranian Journal of Surgery. 2013; 21(1)
_||_1-Avsar FM, Sahin M, Aksoy F, Avsar AF, Aköz M, Hengirmen S, Bilici S. Effects of diphenhydramine HCl and methylprednisolone in the prevention of abdominal adhesions. The American journal of surgery. 2001 Jun 1;181(6):512-5.
BW H. Use of fibrinolytic agents in the prevention of postoperative adhesion formation. Fertil Steril. 2000; 74:203-12.
Emre A, Akin M, Isikgonul I, Yuksel O, Anadol AZ, Cifter C. Comparison of intraperitoneal honey and sodium hyaluronate-carboxymethylcellulose (SeprafilmTM) for the prevention of postoperative intra-abdominal adhesions. Clinics. 2009; 64:363-8.
Ward BC, Panitch A. Abdominal adhesions: current and novel therapies. Journal of Surgical Research. 2011 Jan 1;165(1):91-111.
. Giusto G, Vercelli C, Iussich S, Audisio A, Morello E, Odore R, Gandini M. A pectin-honey hydrogel prevents postoperative intraperitoneal adhesions in a rat model. BMC veterinary research. 2016 Dec; 13:1-5.
Najafian A, Alavi A, Amjadigolpayegani K, Dadipour S, Safarimoradabadi A, Fallahi S, Faramarzi A. Post surgical adhesion prevention: Vitamin C or Satureja Khuzestanica. Veterinary Clinical Pathology The Quarterly Scientific Journal. 2013 May 22;7(1 (25) Spring):1786-92.
Zhang Q, Qiao H, Wu D, Lu H, Liu L, Sang X, Li D, Zhou Y. Curcumin potentiates the galbanic acid-induced anti-tumor effect in non-small cell lung cancer cells through inhibiting Akt/mTOR signaling pathway. Life sciences. 2019 Dec 15; 239:117044.
Chahardoli A, Mavaei M, Shokoohinia Y, Fattahi A. Galbanic acid, a sesquiterpene coumarin as a novel candidate for the biosynthesis of silver nanoparticles: In vitro hemocompatibility, antiproliferative, antibacterial, antioxidant, and anti-inflammatory properties. Advanced Powder Technology. 2023 Jan 1;34(1):103928.
Fatehi Hassanabad A, Zarzycki AN, Jeon K, Deniset JF, Fedak PW. Post-operative adhesions: a comprehensive review of mechanisms. Biomedicines. 2021 Jul 22;9(8):867.
Muir, W.W., Hubbell, J.A.E., Skarda, R.T. and Bednarski, M. Hand book of veterinary anesthesia. 2nd Ed., The Ohio State University College of Veterinary Medicine, Columbus, OH. Mosby,1995, p. 110.
11. Wei G, Wu Y, Gao Q, Shen C, Chen Z, Wang K, Yu J, Li X, Sun X. Gallic acid attenuates postoperative intra-abdominal adhesion by inhibiting inflammatory reaction in a rat model. Medical science monitor: international medical journal of experimental and clinical research. 2018; 24:827.
Abbasian B, Kazemini H, Esmaeili A, Adibi S. Effect of bovine amniotic fluid on intra-abdominal adhesion in diabetic male rats. Journal of Diabetes and its Complications. 2011 Jan 1;25(1):39-43.
Diamond MP, Burns EL, Accomando B, Mian S, Holmdahl L. Seprafilm® adhesion barrier:(2) a review of the clinical literature on intraabdominal use. Gynecological surgery. 2012 Sep;9(3):247-57.
Charboneau AJ, Delaney JP, Beilman G. Fucoidans inhibit the formation of post-operative abdominal adhesions in a rat model. PLoS One. 2018 Nov 21;13(11): e0207797.
Asadi S.Y, Parsai P, Karimi M, Rafiyan Kopaei M. The effect of ethanolic extract of green tea (Camellia Sieneses) on intra-abdominal adhesion following laparotomy in rats. Scientific Journal of Zanjan University of Medical Sciences (Journal of Advances in Medical and Biomedical Research). 2013;21(86):86-96.
16.Iranshahy M, Iranshahi M. Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gum-resin)—A review. Journal of ethnopharmacology. 2011 Mar 8;134(1):1-0.
17. Niazmand R, Razavizadeh BM. Ferula asafoetida: chemical composition, thermal behavior, antioxidant and antimicrobial activities of leaf and gum hydroalcoholic extracts. Journal of Food Science and Technology. 2021 Jun;58(6):2148-59.
18. Goudarzi S, Rassouli FB, Kahrizi D, Shirkhani P, Mahdifar M, Iranshahi M, Rafatpanah H, Keramati MR, Ayatollahi H. Comparing toxicity of galbanic acid, auraptene and umbelliprenin on adult T-cell leukaemia-lymphoma in normoxia and hypoxia. Cellular and Molecular Biology. 2022 Dec 31;68(12):17-20.
19. Sirizi MA, Ghalenoei JA, Allahtavakoli M, Forouzanfar H, Bagheri SM. Anticancer potential of Ferula assa-foetida and its constituents, a powerful plant for cancer therapy. World Journal of Biological Chemistry. 2023 Mar 27;14(2):28-39.
Kim KH, Lee HJ, Jeong SJ, Lee HJ, Lee EO, Kim HS, Zhang Y, Ryu SY, Lee MH, Lü J, Kim SH. Galbanic acid isolated from Ferula assafoetida exerts in vivo anti-tumor activity in association with anti-angiogenesis and anti-proliferation. Pharmaceutical research. 2011 Mar; 28:597-609.
Kohno S, Murata T, Sugiura A, Ito C, Iranshahi M, Hikita K, Kaneda N. Methyl galbanate, a novel inhibitor of nitric oxide production in mouse macrophage RAW264. 7 cells. Journal of natural medicines. 2011 Apr; 65:353-9.
22. Asadi SY, Parsaei P, Karimi M, Rafieian-kopaei M. Effect of ethanolic extract of green tea (Camellia sinensis) on intraabdominal adhesions in rats. J Zanjan Univ Med Sci. 2013;21(86):86-96.
Nazem Zumardi P, Sattari K. Macroscopic and microscopic examination of the anti-adhesion properties of honey and corticosteroids following experimental surgery in the abdominal cavity of rabbits. Clinical studies of large animals. 2022;9(2):24-35.
Davari H, Goharian V, Eghbal A. Experimental study of pentoxifylline in prevention of post-operative intraperitoneal adhesion band. Iranian Journal of Surgery. 2005;13(2)
Mostajeran F, Manouchehri M. Effect of Amniotic Fluid in Treatment of Post-Laparatomy Adhesion Formation in Rat Model. Journal of Isfahan Medical School. 2011;28(121):1668-75.
Pourjafar M, KARIMI I, Farid M, Maghsoudi E, Shakhs NM. The comparison of standard heparin and dalteparin on prevention of adhesion in peritonitis with or without infection in rat. Journal of Veterinary Research. 2007; 62(50:277-280,2007
Panahi F, SHAHRAM E, Khoshmohabat H, Kaka GR, Sadraei S, Hossein AM. Macroscopic and microscopic assessment of normal saline on postoperative adhesion formation in a rat model. Iranian Journal of Surgery. 2013; 21(1)
