Investigation of antimicrobial properties of green synthesized CuO nanostuctures using Berberis integerrima aqueous extract on infected surgical wound caused by Staphylococcus aureus
Subject Areas :
Parvin Eskandari
1
,
Faeze Naghilonia
2
,
Reza Shapouri
3
,
Mahdi Arfaei
4
1 - Department of Chemistry, Zanjan Branch, Islamic Azad University , Zanjan, Iran
2 - Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Gava Zang, Zanjan, Iran
3 - Department of Microbiology,, Zanjan Branch, Islamic Azad University, Zanjan, Iran
4 - Nanobiotechnology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
Received: 2023-05-20
Accepted : 2023-07-19
Published : 2023-07-23
Keywords:
Mice,
Staphylococcus aureus,
Eucerin,
Berberis integerrima,
CuO nanostuctures,
Abstract :
Background & Aims: CuO is one of the most important transition metal oxides due to its interesting properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, photocatalyst, and so on. Staphylococcus aureus bacteria causes a wide range of infectious diseases. In this study, the antimicrobial activity of the synthesized copper oxide nanoparticles in the presence of Berberis integerrima aqueous extract on the infected surgical wound caused by this bacterium was investigated in mice.
Materials and methods: First, copper oxide nanoparticles were synthesized in the presence of Berberis integerrima aqueous extract and confirmed by XRD, FT-IR, FE-SEM analyses, and the MIC and MBC levels of the nanostructures used were determined by the dilution method in Mueller Hinton Broth culture medium. Also, after creating a skin wound and inoculating the solution of Staphylococcus aureus bacteria with a concentration of (5 × 105 CFU/ml), the concentration of MBC along with Eucerin ointment was checked on the infected wounds.
Results: In general, copper oxide nanoparticles synthesized in the presence of Berberis integerrima aqueous extract has a significant antimicrobial activity to control the growth of Staphylococcus aureus bacteria. Also, the synthesized copper oxide has a favorable antimicrobial and reparative effect on the infected surgical wound caused by this bacterium.
Conclusion: According to the findings, copper oxide nanoparticles can be effectively and efficiently synthesized by Berberis integerrima aqueous extract in simple and environmentally friendly conditions. It also has a significant antibacterial effect on Staphylococcus aureus bacteria and has a favorable reparative effect compared to Eucerin ointment. Copper oxide nanoparticles can be expected to play an effective role in the treatment of microbial infections.
Key words: Berberis integerrima, copper nanoparticles, Eucerin, mice, Staphylococcus aureus.
References:
Azam A, Ahmed SA, Oves M, Khan MS, Memic A. Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and Gram–negative bacterial strains. International Journal of Nanomedicine. 2010 Jul 10, 7: 3527-3535.
Rahmani Gohar M, Moslemi HR, Kafshdouzan KH, Mazaheri Nezhad Fard R. Antibacterial activity of origanum vulgare on staphylococcus aureus in a rat model of surgical wound infection. Journal of Medicinal Plants. 2016 Feb 10; 15(57):19-24.
Kazemi N, Arfaei M, Ghasemi M. Antimicrobial and healing effect of Nettle, Purslane and Hedge Nettle extracts on burn infections of Staphylococcus aureus in mice. Journal of Animal Philology and Development. 2022 Dec 19; 16 (1): 59-69. (Persian)
Faheem I, Sammia S, Shakeel AK, Ahmad W, Zaman S. Green synthesis of copper oxide nanoparticles using Abutilon indicum leaf extract: Antimicrobial, antioxidant and photocatalytic dye degradation activities. Tropical Journal of Pharmaceutical Research. 2016 May 4; 16(4): 743-53.
Yallappa S, Manjanna J, Sindhe MA, Satyanarayan ND, Pramod SN, Nagaraja K. Microwave assisted rapid synthesis and biological valuation of stable copper nanoparticles using T. arjuna bark extract. Spectrochim Acta Mol Biomol Spectrosc. 2013 Jun 1; 110:108-115.
Seigneuric R, Markey L, Nuyten DS, Dubernet C, Evelo CT, Finot E, Garrido C. From nanotechnology to nanomedicine: applications to cancer research. Current Molecular Medicine. 2010 Oct 1; 10:640-652.
Liu Z, Kiessling F, Gatjent J. Advanced nanomaterials in multimodel imaging: design, functionalization, and biomedical applications. Journal of Nanomaterials. 2010 Jun; 2010.
Kwak K, Kim C. Viscosity and thermal conductivity of copper oxide nanofluid dispersed in ethylene glycol. Korea Australia Rheology Journal. 2005; 17:35-40.
Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ. Metal oxide nanoparticles as bactericidal agents. Langmuir. 2002 Aug 20; 18(17):6679-6686.
Sankar R, Manikandan P, Malarvizhi V, Fathima T, Shivashangari KS, Ravikumar V. Green synthesis of colloidal copper oxide nanoparticles using Carica papaya and its application in photocatalytic dye degradation. Spectrochim Acta Mol Biomol Spectrosc. 2014 Mar 5; 121:746-50.
Atarod M, Nasrollahzadeh M, Sajadi SM, Green synthesis of Pd/RGO/Fe3O4 nanocomposite using Withania coagulans leaf extract and its application as magnetically separable and reusable catalyst for the reduction of 4-nitrophenol. Journal of Colloid and Interface Science, 2016 Mar 1; 465(1): 249-258.
Nasrollahzadeh M, Sajadi SM. Preparation of Au nanoparticles by Anthemis xylopoda flowers aqueous extract and their application for alkyne/aldehyde/amine A3-type coupling reactions. RSC Adv. 2015; 5: 46240-46246.
Hatamifard A, Nasrollahzadeh M, Lipkowski J. Green synthesis of a natrolite zeolite/palladium nanocomposite and its application as a reusable catalyst for the reduction of organic dyes in a very short time. RSC Advances. 2015; 5: 91372-91381.
Nasrollahzadeh M, Sajadi SM, Maham M. Green synthesis of palladium nanoparticles using Hippophae rhamnoides Linn leaf extract and their catalytic activity for the Suzuki–Miyaura coupling in water. Journal of Molecular Catalysis A: Chemical, 2015 Jan 1; 396: 297-303.
Nasrollahzadeh M, Sajadi SM, Rostami-Vartooni A, Alizadeh M, Bagherzadeh MJ. Green synthesis of the Pd nanoparticles supported on reduced graphene oxide using barberry fruit extract and its application as a recyclable and heterogeneous catalyst for the reduction of nitroarenes. Journal of Colloid and Interface Science, 2016 Mar 15; 466:360-368.
Nasrollahzadeh M, Sajadi SM, Maham M. Tamarix gallica leaf extract mediated novel route for green synthesis of CuO nanoparticles and their application for N-arylation of nitrogen-containing heterocycles under ligand-free conditions. RSC Advances. 2015; 5:40628-40635.
Nasrollahzadeh M, Maham M, Sajadi SM. Green synthesis of CuO nanoparticles by aqueous extract of Gundelia tournefortii and evaluation of their catalytic activity for the synthesis of N-monosubstituted ureas and reduction of 4-nitrophenol. Journal of Colloid and Interface Science, 2015 Oct 1; 455:245-253.
Ahamed M, Alhadlaq HA, Majeed Khan MA, Karuppiah P, Al-Dhabi NA. Synthesis, characterization, and antimicrobial activity of copper oxide nanoparticles. Journal of Nanomaterials. 2014 Jan 1; 2014:17-17.
Timothy C, Birdsall ND, Gregory S, Kelly ND. Berberine: Therapeutic potential of an alkaloid found in several medicinal plants. Alternative medicine review. A Journal of Clinical Therapeutic. 1977; 2(2):94-103.
Khosrokhavar R, Ahmadiani A, Shamsa F. Antihistaminic and anticholinergic activity of methanolic extract of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. Journal of Medicinal Plants. 2010; 9(35):99-105.
Fatehi M, Saleh TM, Fatehi-Hassanabad Z, Farrokhfal K, Jafarzadeh M, Davodi SA. Pharmacological study on Berberis vulgaris fruit extract. Journal of ethnopharmacology. 2005 Oct 31; 102:46-52.
Imanshahidi M, Hosseinzadeh H, Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine. Phytotherapy Research, 2008 Aug; 22(8):999-1012.
Sabir M, Bhide NK. Study of some pharmacological actions of berberine. Indian Journal of Physiology and Pharmacology, 1971 Jul; 15(3):111-32.
Shamsa F, Ahmadiani A, Khosrokhavar R. Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. Journal of Ethnopharmacology, 1999 Feb 1; 64(2):161-166.
Abootorabi Z, Poorgholami M, Hanafi-Bojd MY, Hoshyar, R. Green synthesis of gold nanoparticles using Barberry and Saffron extracts. Modern Care Journal. 2016 Oct 31; 13(4): e13000.
Anzabi, Y. Biosynthesis of ZnO nanoparticles using barberry (Berberis vulgaris) extract and assessment of their physico-chemical properties and antibacterial activities. Green Processing and Synthesis. 2018 Apr 1; 7(2):114–121.
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Oregon; Clinical and Laboratory Standards Institute. 2014.
Shanbhag TV, Sharma C, Adiga S, Bairy LK, Shenoy S, Shenoy G. Wound healing activity of alcoholic extract of Kaempferia galanga in Wistar rats. Indian Journal of Physiology and Pharmacology. 2006 Oct 1; 50(4):384-90.
Parivar K, Yaghmaei P, Hayati RN, Mohammadi MP. Effects of synchronized oral administration and topical application of Kombucha on third-degree burn wounds regeneration in mature rats. Medical Sciences. 2012 Apr 1; 22(1):1-11
Mohamed H. One year prevalence of critically ill burn wound bacterial infections in surgical ICU in Egypt: Retrospective study. Egyptian Journal of Anaesthesia. 2016 Jul 1; 32(3):431-4.
Cullity BD, Elements of X-ray Diffraction, 2nd edition. Addison Wesley Publishing, London. 1978.
Villani M, Alabi AB, Coppede N, Calestani D, Lazzarini L, Zappettini A. Facile synthesis of hierarchical CuO nanostructures with enhanced photocatalytic activity. Crystal Research and Technology. 2014 Aug; 49(8):594-8.
Alizadeh H, Salouti M, Shapouri R. Bactericidal effect of silver nnanoparticles on intramacrophage brucella abortus 544. Jundishapur Journal of Microbiology. 2014 Mar; 7(3): e9039.
Gottenbos B, vander Mei HC, Klatter F, Nieuwenhuis P, Busscher HJ. In vitro and in vivo antimicrobial activity of covalently coupled quaternary ammonium silane coatings on silicone rubber. Biomaterials. 2002 Mar 1; 23(6):1417–23.
Ahani, S, Moayer, F, Khosravi Dehaghi, N. Effect of hydro-alcoholic extract of barberries (Berberis vulgaris) on wound healing process in rats. Journal of Animal Philology and Development. 2023; 16(2): 12-26. (Persian)
Ghahremani, F, Izanloo, C. Green synthesis of copper oxide nanopartcles using extract of Hypericum perforatum and Marrubium vulgare and evaluation of antioxidant properties of herbal extracts and antibacterial feature of green-synthesized nanostructures. Journal of Nanomaterials. 2020; 12(44): 239-249. (Persian)
Ebrahimi, K. Synthesis of copper nanoparticles using aqueous extract of Postia puberula flora and evaluation of their antimicrobial activity. Cellular and Molecular Research (Iranian Journal of
Biology), 2018 Dec 22; 31(4): 437-445. (Persian)
Shankar, Sh, Jong-Whan, R. Effect of copper salts and reducing agents on characteristics and antimicrobial activity of copper nanoparticles. Materials Letters. 2014; 132:307–311
Murugan ,V, Sadhasivam, S. Glucosamine functionalized copper nanoparticles: preparation, characterization and enhancement of anti-bacterial activity by ultraviolet irradiation. Chemical Engineering Journal. 2012 Oct 1; 209:558-567.
Amjady, F, Golestani, B, Karimi, F. An investigation of the effect of copper oxide nanoparticles on E. coli genome by RAPD molecular markers. Cellular and Molecular Research (Iranian Journal of Biology), 2016 Feb 20; 28(4): 475-487. (Persian)
Golestani B, Jafari A, karimi F. An investigation of the effect of copper oxide nanoparticles on Salmonella tifi morum genome by RAPD molecular markers. Modares Journal of Biotechnology, 2016 Sep 10; 7 (2) :80-90. (Persian)
_||_
Azam A, Ahmed SA, Oves M, Khan MS, Memic A. Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and Gram–negative bacterial strains. International Journal of Nanomedicine. 2010 Jul 10, 7: 3527-3535.
Rahmani Gohar M, Moslemi HR, Kafshdouzan KH, Mazaheri Nezhad Fard R. Antibacterial activity of origanum vulgare on staphylococcus aureus in a rat model of surgical wound infection. Journal of Medicinal Plants. 2016 Feb 10; 15(57):19-24.
Kazemi N, Arfaei M, Ghasemi M. Antimicrobial and healing effect of Nettle, Purslane and Hedge Nettle extracts on burn infections of Staphylococcus aureus in mice. Journal of Animal Philology and Development. 2022 Dec 19; 16 (1): 59-69. (Persian)
Faheem I, Sammia S, Shakeel AK, Ahmad W, Zaman S. Green synthesis of copper oxide nanoparticles using Abutilon indicum leaf extract: Antimicrobial, antioxidant and photocatalytic dye degradation activities. Tropical Journal of Pharmaceutical Research. 2016 May 4; 16(4): 743-53.
Yallappa S, Manjanna J, Sindhe MA, Satyanarayan ND, Pramod SN, Nagaraja K. Microwave assisted rapid synthesis and biological valuation of stable copper nanoparticles using T. arjuna bark extract. Spectrochim Acta Mol Biomol Spectrosc. 2013 Jun 1; 110:108-115.
Seigneuric R, Markey L, Nuyten DS, Dubernet C, Evelo CT, Finot E, Garrido C. From nanotechnology to nanomedicine: applications to cancer research. Current Molecular Medicine. 2010 Oct 1; 10:640-652.
Liu Z, Kiessling F, Gatjent J. Advanced nanomaterials in multimodel imaging: design, functionalization, and biomedical applications. Journal of Nanomaterials. 2010 Jun; 2010.
Kwak K, Kim C. Viscosity and thermal conductivity of copper oxide nanofluid dispersed in ethylene glycol. Korea Australia Rheology Journal. 2005; 17:35-40.
Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ. Metal oxide nanoparticles as bactericidal agents. Langmuir. 2002 Aug 20; 18(17):6679-6686.
Sankar R, Manikandan P, Malarvizhi V, Fathima T, Shivashangari KS, Ravikumar V. Green synthesis of colloidal copper oxide nanoparticles using Carica papaya and its application in photocatalytic dye degradation. Spectrochim Acta Mol Biomol Spectrosc. 2014 Mar 5; 121:746-50.
Atarod M, Nasrollahzadeh M, Sajadi SM, Green synthesis of Pd/RGO/Fe3O4 nanocomposite using Withania coagulans leaf extract and its application as magnetically separable and reusable catalyst for the reduction of 4-nitrophenol. Journal of Colloid and Interface Science, 2016 Mar 1; 465(1): 249-258.
Nasrollahzadeh M, Sajadi SM. Preparation of Au nanoparticles by Anthemis xylopoda flowers aqueous extract and their application for alkyne/aldehyde/amine A3-type coupling reactions. RSC Adv. 2015; 5: 46240-46246.
Hatamifard A, Nasrollahzadeh M, Lipkowski J. Green synthesis of a natrolite zeolite/palladium nanocomposite and its application as a reusable catalyst for the reduction of organic dyes in a very short time. RSC Advances. 2015; 5: 91372-91381.
Nasrollahzadeh M, Sajadi SM, Maham M. Green synthesis of palladium nanoparticles using Hippophae rhamnoides Linn leaf extract and their catalytic activity for the Suzuki–Miyaura coupling in water. Journal of Molecular Catalysis A: Chemical, 2015 Jan 1; 396: 297-303.
Nasrollahzadeh M, Sajadi SM, Rostami-Vartooni A, Alizadeh M, Bagherzadeh MJ. Green synthesis of the Pd nanoparticles supported on reduced graphene oxide using barberry fruit extract and its application as a recyclable and heterogeneous catalyst for the reduction of nitroarenes. Journal of Colloid and Interface Science, 2016 Mar 15; 466:360-368.
Nasrollahzadeh M, Sajadi SM, Maham M. Tamarix gallica leaf extract mediated novel route for green synthesis of CuO nanoparticles and their application for N-arylation of nitrogen-containing heterocycles under ligand-free conditions. RSC Advances. 2015; 5:40628-40635.
Nasrollahzadeh M, Maham M, Sajadi SM. Green synthesis of CuO nanoparticles by aqueous extract of Gundelia tournefortii and evaluation of their catalytic activity for the synthesis of N-monosubstituted ureas and reduction of 4-nitrophenol. Journal of Colloid and Interface Science, 2015 Oct 1; 455:245-253.
Ahamed M, Alhadlaq HA, Majeed Khan MA, Karuppiah P, Al-Dhabi NA. Synthesis, characterization, and antimicrobial activity of copper oxide nanoparticles. Journal of Nanomaterials. 2014 Jan 1; 2014:17-17.
Timothy C, Birdsall ND, Gregory S, Kelly ND. Berberine: Therapeutic potential of an alkaloid found in several medicinal plants. Alternative medicine review. A Journal of Clinical Therapeutic. 1977; 2(2):94-103.
Khosrokhavar R, Ahmadiani A, Shamsa F. Antihistaminic and anticholinergic activity of methanolic extract of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. Journal of Medicinal Plants. 2010; 9(35):99-105.
Fatehi M, Saleh TM, Fatehi-Hassanabad Z, Farrokhfal K, Jafarzadeh M, Davodi SA. Pharmacological study on Berberis vulgaris fruit extract. Journal of ethnopharmacology. 2005 Oct 31; 102:46-52.
Imanshahidi M, Hosseinzadeh H, Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine. Phytotherapy Research, 2008 Aug; 22(8):999-1012.
Sabir M, Bhide NK. Study of some pharmacological actions of berberine. Indian Journal of Physiology and Pharmacology, 1971 Jul; 15(3):111-32.
Shamsa F, Ahmadiani A, Khosrokhavar R. Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. Journal of Ethnopharmacology, 1999 Feb 1; 64(2):161-166.
Abootorabi Z, Poorgholami M, Hanafi-Bojd MY, Hoshyar, R. Green synthesis of gold nanoparticles using Barberry and Saffron extracts. Modern Care Journal. 2016 Oct 31; 13(4): e13000.
Anzabi, Y. Biosynthesis of ZnO nanoparticles using barberry (Berberis vulgaris) extract and assessment of their physico-chemical properties and antibacterial activities. Green Processing and Synthesis. 2018 Apr 1; 7(2):114–121.
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Oregon; Clinical and Laboratory Standards Institute. 2014.
Shanbhag TV, Sharma C, Adiga S, Bairy LK, Shenoy S, Shenoy G. Wound healing activity of alcoholic extract of Kaempferia galanga in Wistar rats. Indian Journal of Physiology and Pharmacology. 2006 Oct 1; 50(4):384-90.
Parivar K, Yaghmaei P, Hayati RN, Mohammadi MP. Effects of synchronized oral administration and topical application of Kombucha on third-degree burn wounds regeneration in mature rats. Medical Sciences. 2012 Apr 1; 22(1):1-11
Mohamed H. One year prevalence of critically ill burn wound bacterial infections in surgical ICU in Egypt: Retrospective study. Egyptian Journal of Anaesthesia. 2016 Jul 1; 32(3):431-4.
Cullity BD, Elements of X-ray Diffraction, 2nd edition. Addison Wesley Publishing, London. 1978.
Villani M, Alabi AB, Coppede N, Calestani D, Lazzarini L, Zappettini A. Facile synthesis of hierarchical CuO nanostructures with enhanced photocatalytic activity. Crystal Research and Technology. 2014 Aug; 49(8):594-8.
Alizadeh H, Salouti M, Shapouri R. Bactericidal effect of silver nnanoparticles on intramacrophage brucella abortus 544. Jundishapur Journal of Microbiology. 2014 Mar; 7(3): e9039.
Gottenbos B, vander Mei HC, Klatter F, Nieuwenhuis P, Busscher HJ. In vitro and in vivo antimicrobial activity of covalently coupled quaternary ammonium silane coatings on silicone rubber. Biomaterials. 2002 Mar 1; 23(6):1417–23.
Ahani, S, Moayer, F, Khosravi Dehaghi, N. Effect of hydro-alcoholic extract of barberries (Berberis vulgaris) on wound healing process in rats. Journal of Animal Philology and Development. 2023; 16(2): 12-26. (Persian)
Ghahremani, F, Izanloo, C. Green synthesis of copper oxide nanopartcles using extract of Hypericum perforatum and Marrubium vulgare and evaluation of antioxidant properties of herbal extracts and antibacterial feature of green-synthesized nanostructures. Journal of Nanomaterials. 2020; 12(44): 239-249. (Persian)
Ebrahimi, K. Synthesis of copper nanoparticles using aqueous extract of Postia puberula flora and evaluation of their antimicrobial activity. Cellular and Molecular Research (Iranian Journal of
Biology), 2018 Dec 22; 31(4): 437-445. (Persian)
Shankar, Sh, Jong-Whan, R. Effect of copper salts and reducing agents on characteristics and antimicrobial activity of copper nanoparticles. Materials Letters. 2014; 132:307–311
Murugan ,V, Sadhasivam, S. Glucosamine functionalized copper nanoparticles: preparation, characterization and enhancement of anti-bacterial activity by ultraviolet irradiation. Chemical Engineering Journal. 2012 Oct 1; 209:558-567.
Amjady, F, Golestani, B, Karimi, F. An investigation of the effect of copper oxide nanoparticles on E. coli genome by RAPD molecular markers. Cellular and Molecular Research (Iranian Journal of Biology), 2016 Feb 20; 28(4): 475-487. (Persian)
Golestani B, Jafari A, karimi F. An investigation of the effect of copper oxide nanoparticles on Salmonella tifi morum genome by RAPD molecular markers. Modares Journal of Biotechnology, 2016 Sep 10; 7 (2) :80-90. (Persian)
