Study of the effect of safflower(carthamus tinctorius) hydroalcoholic extract on renal function in male Wistar rats

Subject Areas :

Ameneh Khoshvaghti ¹ ( Department of Clinical Sciences, Faculty of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran )
Mohammad Bizaval ² ( Graduated of Faculty of Veterinary Medicine Kazerun Branch, Islamic Azad University, Kazerun, Iran )

Received: 2023-09-08 Accepted : 2023-10-18 Published : 2023-10-23

Keywords: Renal function, hydroalcoholic extract, Safflower, Rat,

Abstract :

Background & Aims: Safflower as a chromogen plant can be used in food industry instead of synthetic colorful compound and saffron as well. This plant has some useful properties such as anti-inflammation. But there are no published researches about useful and harmful effects of this plant on renal function. Therefore this study was done to determine this point. Materials & Methods: 40 wistar male rats were selected and divided into 4 groups (control and 3 experimental groups). The experimental groups 1,2 and 3 received 100, 200 and 300mg/kg/B.W of the extract respectively as subcutaneously for 28days . Blood sample was obtained at days 14 and 28 .After separating serum uric acid, creatinine and BUN were measured by colorimetrically methods. Potassium and sodium were assayed by photometrically methods. Results: There were no significant statistical differences between the mean concentration of creatinine and uric acid in experimental groups and control group in two terms of study. But there were significant statistically differences between the mean concentration of urea nitrogen of some experimental groups in comparison to control group and also between the mean levels of sodium and potassium in all three experimental groups in comparison to control group in days 14 and 28. Conclusion: Due to the serum creatinine and uric acid concentration don’t changes statistically we can resulted the different doses of Safflower  hydroalcoholic extract have no effect on renal function in short, or long term, so can be used this plant, without any concern with it's renal complications in long term.

References:

1. Delshad E, Yousefi M, Sasannezhad P, Rakhshandeh H, Ayati Z. Medical uses of Carthamus tinctorius L. (Safflower): a comprehensive review from Traditional Medicine to Modern Medicine.Electron Phys 2018;10(4):6672-6681.

2.Khoshvaghti A, Abtahi M.The effects of safflower (Carthamus tinctorius) extract on thyroid gland activity in rats. J Bas Clin Path 2020 ;8(2):22-27.

3.Deylamghani J , Gharibzadeh Sh. Human Physiology. Tehran   university of  medical sciences.2004;426-475.( Persian)              

4. Shier D,Butler J, lewis R. Laboratory Manual for Hole,s Essentials of human anatomy and physiology .McGraw Hill Science/Engineering/Math , 12^th ed.2014; P.467-488.

5.Anthony McG. Anat physiol Bowel  2005; pp:1-5.

6.Abdelhalim MAK, Abdelmoussa SA. The gold nanoparticle size and expasureduration effect on the liver and kidney function of rats:In vivo. Saudi J Biol sci 2013 ;20(2):177-8.

7. Guyton C,Hall E. Text book of medical physiology. Elsevier.12^th ed.2011; chapter 26; 307-325.


8. Carl A, Edward R, David E. Tietz Fundamental of Clinical Chemistry. Diwcmr: Andrew Allen.2008;P.632-636.


9. Lulich JP,Osborne CA .Interpretation of urine protein-ceratinine ralios in dogs with glomerular and nonglomerular disorders. Compendium on continuing education for the practicing veterinarian 1990;12 :69-72.


10. Uma pradeed K, Geervani P, Eggum Bo .Common Indian spices nutrient composition consumption and contribution to dietary value . Plant foods Hum Nutr 1993;44(2):137-148.

11.Verma RJ, Asnani V. Ginger extract ameliorates parable induced biochemical changes in liver and kidney of mice . Acta pol pharm 2007; 64(3):217-220.

12.Murahashi SHI. Ruthenium in organic syntheses . Wiley‐VCH Verlag GmbH & Co. KGaA. 2004. DOI:10.1002/3527603832

13. Zhang XD, Wu HY,  Wu D ,  Wang  YY,  Chang JH,  Zhai ZB,  Meng AM,  Liu PX,  Zhang LA, Fan FY. Toxicologic effects of gold nanoparticles in vivo by different administration .Int J Nanomedicine 2010;7:771-781


14. Ghasemi A, Dehkordi N. Iranian herbal pharmacopoeia. Tehran Ministry of Health and Medical Education.2002; 24-5 (Persian).


15. Aeenehchi Y. Iran Medicinal plants.Tehran University.1992;1042-3. (Persian)


16. Tietze NW. Fundumental of Clinical Chemistry. 3^th ed. W.B Saunders.1987.


17. Stockhom SL, Scott MA. Fundamental of Veterinary Clinical Pathology. Saunders. 2002; pp:317-528.


18. Alavash-Shooshtari A, Khorsandi LS, Ahmadi Kh. Protective effect of Carthamus tinctorius L.extract on acetaminophen induced nephrotoxicity in mice. J Gorgan Uni Med Sci 2016; 18(3): 28-33. (Persian)


19. Gao F, Wu XH, Luo CL, He YF, Zhang LS, Yang M. Effect of saffor (Carthamus tinctorius) injection on renal ischemia/reperfusion injury in rats. Zhongguo Zhong Yao Za Zhi 2006;31(21):1814-1818. ( Chinese)


20. Yang YL, Chang SY, Teng HC, Liu YS, Lee TC, Chuang LY. Safflower extract: a novel renal fibrosis antagonist that functions by suppressing autocrine TGF-beta. J Cell Biochem 2008; 104(3):908-19. doi: 10.1002/jcb.21676.


21. Lin WC, Lai MT, Chen HY, Ho CY, Man KM, Shen JL. Protective effect of Flos carthami extract against ethylene glycolinduced urolithiasis in rats. Urol Res 2012; 40(6):655-61. doi:10.1007/s00240-012-0472-4.


22. Qazi N, Khan RA, Rizwani GHH , Feroz Z. Effect of Carthamus tinctorius (Safflower) on fasting blood glucose and insulin levels in alloxan induced diabetic rabbits Pak J Pharm Sci 2014 ; 27(2):377-380.