investigation of sirtuin activity in diabetic and caloric restricted rat and effect of nitric oxide on it

Subject Areas : blood biochemistry & investigation of biomarkers

safar mohseni bandpay ¹ (golestan university)
nazanin nazari ² (golestan university-faculty of science-biology department)

Received: 2023-04-16 Accepted : 2023-07-29 Published : 2024-05-22

Keywords: Nitric oxide, diabetes, calorie restriction, sirtuin,

Abstract :

Background & Aim: Sirtuins are category 3 proteins of a family of histone deacetylase enzymes that catalyze the hydrolysis of the acetylated end of histone, causing chromatin shape change, creating heterochromatin, and as a result inhibiting transcription, and plays a role in aging and cancer. Sirtuin regulates important cellular processes such as apoptosis, cell aging and metabolism. Therefore, sirtuin can be a new therapeutic target for diabetes. Also, sirtuin is known as a protein that regulates lifespan. In this study, the effect of nitric oxide on sirtuin activity in diabetic rats under caloric restriction was investigated.

Materials & Methods: The animals used in this study were male Wistar rats weighing 250 grams, which were divided into 5 groups: control (C), calorie restricted (CR), diabetic (D), L-Arginine and L-Name groups. And each group included ten animals. To make each animal diabetic, streptozocin with a dose of 50 mg/kg was injected intraperitoneally. The group receiving L-Name (NO inhibitor) received it at a dose of 10 mg/kg as an intra-abdominal injection. The L-arginine (precursor of nitric oxide) group also received a dose of 50 mg/kg as an intraperitoneal injection.

Results: The calorie restriction group was subjected to a low calorie diet for 4 weeks. After a period of 4 weeks, the animals were anesthetized and blood was taken directly from their hearts. The amount of sirtuin was measured by the relevant kit and with the Elizarider device. The results were analyzed using spss software. The amount of serum sirtuin increased significantly in the L-arginine (P<0.05) and CR (P<0.01) groups, while it decreased significantly in the diabetes group (P<0.001). Also, the reduction of sirtuin in the L-Name group was not significant.

Conclusion: The results showed that L-arginine, as a precursor of nitric oxide, increases the amount of sirtuin in diabetic rats under caloric restriction.

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