Isolation and identification of Bacillus subtilis and Bacillus coagulans from intestines of local chickens and its effect on ctxm and luxs gene expression in Escherichia coli pathogenic isolates

Subject Areas :

Zahra  Elahianfiroz ¹ (Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran)
Majid  baseri salehi ² (Department of microbiology, kazeoun branch, Islamic azad university, kazeoun, Iran)
Masoud  Ghane ³ (Department of Microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran)

Received: 2021-08-10 Accepted : 2022-01-20 Published : 2022-02-20

Keywords: Escherichia coli, Ctxm, Bacillus subtilis, Luxs, probiotics, Bacillus coagulans,

Abstract :

Inroduction & Objective: The aim of this study was to investigate the effects of probiotics isolated from local chickens on the expression of luxS, ctxM genes in resistant Escherichia coli.  Material and Methods: Biochemical tests of Escherichia coli samples were isolated and then the presence of luxS and ctxM genes was detected by PCR with specific primers. In order to extract the native strains of Bacillus coagulans and Bacillus subtilis, the intestinal contents of 9 local chickens were cultured, isolated and identified by biochemical and PCR methods. Results: Commercial strains of Bacillus subtilis and Bacillus coagulans were isolated along with isolates of resistant Escherichia coli strains containing ctxm and luxs genes. Real-time PCR was used to evaluate gene expression. From 300 fecal samples taken, 40 isolates(7.5%) of Escherichia coli were obtained; Four Escherichia coli strains were isolated from patients carrying ctxM and luxS genes. Isolates of Bacillus coagulans and Bacillus subtilis were confirmed by molecular experiments. Decreased by 2.2 times, equal to the control group in Escherichia coli. Conclusion: The results of statistical analysis showed that there was a significant relationship between the presence of native and commercial probiotics in culture and reduced expression of ctxM and luxS genes.

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