The Effect of the Ibuprofen on the Genetic Changes of Zebrafish (Danio rerio) Using QPCR Methods
Subject Areas :مهسا افشاری کاشانیان 1 , پرگل قوام مصطفوی 2 , علی ماشینچیان مرادی 3
1 - دانشگاه علوم و تحقیقات
2 - دانشگاه علوم و تحقیقات
3 - دانشگاه علوم و تحقیقات
Keywords: qPCR, Zebrafish, Ibuprofen, P53 Gene,
Abstract :
Inroduction & Objective: Ibuprofen, as a drug contaminant, has deleterious and in some cases irreversible effects on aquatic organisms and in general on living things, which in many cases can result in genetic damage and damage to the DNA structure. The effects of different concentrations of ibuprofen on DNA damage and damage to zebrafish are investigated. In this study, genetic changes of p53 gene and its expression in zebrafish after two weeks of exposure to 0.1, 1 and 10 mg / l ibuprofen in vitro were investigated. Material and Method: In this study zebrafish were exposed to the above mentioned concentrations for two weeks and then RNA extracted from them. The results were analyzed using QPCR method. Results: The low drug, 0.1 mg / l had no effect on p53 gene expression, while the other two concentrations, which were 1 and 10 mg / l ibuprofen, respectively, showed a significant difference between the control and the drug containing sample. As a result, the p53 gene was highly expressed. Also for the internal control gene that was GAPDH, there was no significant difference at the lowest drug concentration. Ray did not show a significant difference between the treatment and control samples, while in the subsequent treatments there was a significant difference between control and treatment samples.The final result of this study was that p53 gene expression increased 1.09, 0.57 and 2.2 fold in sample with lowest drug concentration, mean concentration and highest drug concentration, respectively.
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