The Effect of Zolpidem on Uterus Tissue Histological Changes and Estradiol Serum of Adult NMRI Mouse Strain
Subject Areas :Samaneh Mohammadian Kondori 1 , Nasim Hayati Rodbari 2 , Simin Mohammadi Gorji 3 , Kazem Parivar 4
1 - دانشگاه آزاد اسلامی،واحد علوم و تحقیقات، کارشناس ارشد گروه زیست شناسی، تهران. ایران.
2 - دانشگاه آزاد اسلامی،واحد علوم و تحقیقات، کارشناس ارشد گروه زیست شناسی، تهران. ایران.
3 - دانشگاه آزاد اسلامی واحد ساری، استادیار گروه زیست شناسی، ساری. ایران.
4 - دانشگاه آزاد اسلامی،واحد علوم و تحقیقات، کارشناس ارشد گروه زیست شناسی، تهران. ایران
Keywords: Keywords:Zolpidem, estradiol, tissue, Uterus, NMRI mouse strain,
Abstract :
Inroduction & Objective: Zolpidem (with the brand name of Ambien) is a non-benzodiazepine hypnotic which binds to the benzodiazepine binding site on the GABA-A receptors given that this drug is used in more puberty so.The aim of this study was the effect of zolpidem on reproductive system of female adult NMRI mouse.Material and Methods:In this experimental study thirty adult female mice NMRI strain at a mean weight of 30±26 grams were divided into five groups Zolpidem solution was prepared in distilled water at 5, 10, and 20 (mg/kg of body weigh) doses, and 0.5 cc injections were done intraperitoneally every day for 14 days The con trol group received no injection. The sham group received distilled water (as solvent of zolpidem) and treatment groups of 1, 2, and 3 received doses of 5, 10, and 20 mg/kg The treatment groups were sacrificed one day after the last injection, and their hearts were dissected and blood samples were obtained.The concentrations of the hormones were measured by the ELISA testThe uterus tissue was separated and examined after the process of alcohol supply, molding, shredding as well as Hematoxylin and eosin painting and the results were evaluated via the tukey-test, ANOVA by SPSS program.Results:The results showed a significant decrease in the mean serum, Estradiol in the experimental groups compared to the sham and control histological studies of sections showed the diameter of endometrium and uterus lumen, decreased in experimental groups compared to the sham and control Also significant decrease the number of uterus glands in experimental groups 3 compared to the sham and control.Conclusion:Injection of zolpidem significantly is effective on ,uterus tissue and concentration of Estradiol hormone.
