Histological,Molecular Examination and Nano-Garaphene Oxide and Antibiotic Synergistic Effect on E.coli Isolated from of Colorectal Cancer
Subject Areas :Sedighe Mehrabian 1 , Azadeh Behzady 2 , Shahla Mohamad Ganji 3
1 - استاد گروه میکروبیولوژی، دانشکده زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی و دانشگاه خوارزمی، تهران. ایران.
2 - کارشناسی ارشد بیوتکنولوژی میکروبی، دانشکده زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران. ایران.
3 - استادیار ژنتیک مولکولی پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری تهران ایران.
Keywords: Synergism .Graphene Oxide. Escherishiacoli . Colorectal Cancer,
Abstract :
Inroduction & Objective: One of the most important factors for colorectal cancer is bacterial infection. Infection with certain types of E.coli bacteria in patients with inflammatory bowel disease, colorectal cancer can be started.The bacteria produce toxins general secretary coliBaktyn as secondary metabolites intestinal bacteria microbiomeconditions provide for their pathogenicity. With impaired cell cycle triggers the development of colorectal cancer .The aim of this study due to bacteria becoming resistant E.colipks has colibaktyn, the use of nano antibiotics to eliminate the infection and ultimately prevent the development of colorectal cancer.Material and Method60Colon biopsies from patients referred to the clinic,30 samples from colorectal cancer patients and 30 samples from healthy subjects bowel disease and colon cancer, genes ofclbNclbBpks was a marker of genomic islands. Two samples isolated from patients and healthy individuals affected by E.coli pks common antibiotics treat intestinal and nanographene oxide inhibition diameters were measured using nano synergistic effect of antibiotics on the bacteria was measured. Results: The E.coli bacteria isolated from biopsy of colorectal cancer patients in the control group who were contained both genes meaning fulconection between the prevalence of bacteria E.coli contain clbB and clbN gene and colorectal cancer not founded. Bacteria isolated from colorectal cancer than the control group showed greater resistance to antibiotics. The synergism between antibiotics and graphene oxide nanoparticles were observed.Conclusions:The results of these genes in Iranian society comparedwith other communities research shows less frequent E.coli bacteria isolated from colorectal cancer resistant than the control sample
