Effect of Hydroalcoholic Extract of Berberis integerrima FruitOn Function and Liver Tissue in Adult Male Rat’s Treatment with Ethanol
Subject Areas :Shiva Shakurunia 1 , Mehrdad Shariati 2
1 - دانشجوی کارشناسی ارشد، گروه بیولوژی، واحدکازرون، دانشگاه آزاد اسلامی،کازرون. ایران.
2 - دانشیار، گروه بیولوژی، واحدکازرون، دانشگاه آزاد اسلامی، کازرون. ایران
Keywords: Rat, ethanol, liver, Berberis integerrima,
Abstract :
Inroduction & Objective:Nowadays consuming alcohol causes diseases and different disorders ,specially liver disorder.The purpose of this survey is to study the effect of berberis integerrima’s fruit on liver enzymes,serum level (ALT, AST, ALP,GGT), albumin scale and total protein and changes in liver tissue caused by consuming alcohol.Material and Methods:: In this study, ethanol was used to apply liver damage.50 male Wistar rats(160-220 gr) were randomly divided into 5 groups as follows:1)Control group without any treatment,2)Positive sham group receiving 2 ml of 20% ethanol daily by gavage ,3)Negative sham group receiving berberis integerrima dose(200 mg/kg bw) and experimental groups1 and 2 receiving 20% ethanol respectively for 21 days and in the second 28days,100mg/ kg and 200 mg / kg doses of berberis integerrimawere prescripted ,Then blood samples,were taken to measure,liver enzyme ,Albumin and the Total proteinlevels.Histological section for histopathological studies of liver were provided.Results: The results showed that changes in body and liver weight in sham groups were not significant in comparison to control groups. In enzymes like AST, ALP, GGT,ALT,Albumin and total protein in exprimental groups receiving Berberis integerrima were significant atp≤./5 level in compare to positive sham group that was treated with ethanol.Histological studies shows that in the positive sham group that was treated with ethanol large areas of empty hepatocyte cells were seen, and the recovery processes were observed in the experimental groups receiving Berberis integerrima’s extractConclusion: Because of having anti-oxidative compounds and more flavonord Consuming berberis integerrima extract in (200 mg/kg) dose protects liver cell against oxidative stress caused by ethanol
