The Role Of TNF-α in the Pattern Of CXCR4 Gene Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells
Subject Areas :Rozita Ziaei 1 , Maryam Ayatollahi 2 , رامین یعقوبی 3 , زینب صحرائیان 4 , نصرت الله ضرغامی 5
1 - دانشگاه علوم پزشکی تبریز، شعبه بین الملل ارس. جلفا. ایران.
2 - دانشیار، دانشگاه علوم و پزشکی شیراز، مرکز تحقیقات پیوند و ترمیم اعضاء بیمارستان نمازی، شیراز. ایران
3 - دانشیار، دانشگاه علوم و پزشکی شیراز، مرکز تحقیقات پیوند و ترمیم اعضاء بیمارستان نمازی، شیراز. ایران.
4 - دانشگاه آزاد اسلامی، ,واحدزنجان. گروه میکروبیولوژی ، زنجان .ایران.
5 - ستاد، دانشگاه علوم پزشکی تبریز ، دانشکده علوم نوین، تبریز، ایران.
Keywords: Mesenchymal stem cells, CXCR4, Pattern of Gene Expression, Tumor Necrosis Factor-α,
Abstract :
Inroduction and ObjectiveMigration and engraftment have a vital role in tissue regeneration and transplantation and CXCR4 is a known chemokin receptor in making and increasing homing and engraftment of cells into damaged tissue or wounds. CXCR4 expresses in low level on human MSCs, but we show here that CXCR4 expression by hBM MSCs up-regulates through exposure to TNF-α in a dose and time dependent manner.Materials and Methods:Cultured hBM MSCs were divided into 9 groups. One group was un treated and the others treated with 1 ng/ml and 10 ng/ml TNF- α in 2, 10, 24 and48 hours. Total mRNA was extracted and cDNA synthesized. Then rate of CXCR4 expression compared with housekeeping gene (β-actin) by Real Time PCR.Results: In untreated hMSCs CXCR4 expressed in low level. In treated hMSCs with 1ng/ml TNF-α in 2, 10, 24 and 48 hours CXCR4 expression was increased respectively 32, 64, 256,32 fold and with 10ng/ml increased 16, 32, 512, 32 fold. The highest level of CXCR4 gene expression found after treatment with 10ng/ml TNF-a in 24h was highly increased(2-∆∆Ct = 512 ).Conclusion:These finding indicate that in vitro control of both dose and time factors may be important in future-stem cell transplantation therapies
