Comparison of the Efficiency of Iodine Solution, Methylene Blue and Potassium Permanganate for Disinfection of Stellate Sturgeon Acipenser stellatus(Pallas, 1771) Eggs
Subject Areas :Abdolali Rahdari 1 , Bahram Falahatkar 2 , Hossein Mohammadi 3 , Samad Darvishi 4
1 - دانشجوی دکتری، گروه شیلات، دانشکده منابع طبیعی، دانشگاه گیلان، صومعه سرا، گیلان. ایران.
2 - استاد، گروه شیلات، دانشکده منابع طبیعی، دانشگاه گیلان، صومعه سرا، گیلان. ایران
3 - مجتمع تکثیر و بازسازی ذخایر ماهیان خاویاری شهید دکتر بهشتی، رشت، گیلان. ایران.
4 - مجتمع تکثیر و بازسازی ذخایر ماهیان خاویاری شهید دکتر بهشتی، رشت، گیلان. ایران.
Keywords: Artificial Breeding, Iodine, Egg Disinfection, Stellate Sturgeon, potassium permanganate, Methylene Blue,
Abstract :
Inroduction & Objective:Considering the importance of stellate sturgeon fish as endangered species, the artificial breeding plays an important role in the restoration of natural resources of this fish in the Caspian Sea. One of the essential activities in the incubation period is disinfection of eggs, which if done correctly can increase survival and larvae production. Therefore, finding the best approach for disinfection of stellate sturgeon eggs is so necessary.Material and Methods:Eggs were artificially fertilized and subjected to a bath dip treatment in given concentrations of the above chemicals before being incubating. Prior to treatment, the eggs were splited into three disinfected groups (100 mg L-1 iodophor, 15 min; 20 mg L-1 potassium permanganate, 30 min exposure and 100 mg L-1 methylene blue; 2 hours and) and non-disinfected group (control). Then, the eggs were transferred to Yushchenko incubators for incubation. During the incubation period, fungal and dead eggs were separate and counted physically every day. After hatching, the numbers of larvae per box were counted separately.Results: Eggs that disinfected with iodine solution and methylene blue were less likely to fungal infection and their conversion to larvae was higher than eggs that disinfected with potassium permanganate and the control group.Conclusion: The study showed that using iodine solution to disinfect stellate sturgeon is more effective and easier than some other disinfectants.
