Breast Cancer Phothothermal Therapy by Using Gold Nanorods and Tissue Distribution of Gold Nanoparticles in Mouse Model
Subject Areas :محسن کلانتری 1 , کاظم پریور 2 , مجتبی Salouti 3 , مهرداد حمیدی 4 , سعید عمادی 5
1 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
3 - گروه میکروبیولوژی، دانشکده علوم، واحد زنجان، دانشگاه آزاد اسلامی، زنجان، ایران
4 - مرکز تحقیقات نانوتکنولوژی داروئی، دانشگاه علوم پزشکی زنجان، زنجان، ایران.
5 - دانشکده علوم زیستی، مرکز تحصیلات تکمیلی در علوم پایه، زنجان، ایران.
Keywords: Near-Infrared (NIR) laser, gold nanorods, breast cancer, photothermal therapy,
Abstract :
v:* {behavior:url(#default#VML);} o:* {behavior:url(#default#VML);} w:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);} >1024x768Normal0falsefalsefalseEN-USX-NONEAR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif"; mso-bidi-font-family:"B Nazanin";} >Inroduction & ObjectiveTreatment methods for breast cancer are not specific and each one has its own drawbacks. For this reason, scientists are seeking ways of which specifically affect cancer cells. Photothermal therapy is a method that uses near-infra red (NIR) laser energy to create sufficient heat to destroy cancer cells. In this study, the photothermal effect of gold nanorods (GNRs) was investigated for breast cancer treatment in vitroMaterials and Methods:GNRs with peak absorption of 808 nm were synthesized and coated with Poly ethilen glycol (PEG). After confirming the characteristics of the prepared complex, the therapeutic effect of new agent was studied on SK-BR-3 cell line by using the NIR laser.ResultsThe cytotoxcicity assay showed the biocompatibility of PEGylated GNRs complex. Through the in vitro photothermal study, significant cell death was observed in breast cancer cells incubated with the complex along with the laser irradiation. The biodistribusion study showed the appropriate localization of new agent in vital organs after 24 h in mouse model. ConclusionIn summary, the present study reports the successful treatment of Breast cancer cells by the prepared photothermal agent using. Labeling of gold nanoparticles(such as antibodies) can improve the treatment efficiency and prevent damage to healthy cells that need further studies.
