Molecular Identification and Phylogeny of16Species of Bivalvia on Shores of the Persian Gulf(Hengam Island,Larak Island,Geshm Island,Lenge Island)
Subject Areas :Shohreh Masaeli 1 , Pargol Ghavam Mostafavi 2 , Homauon Hosseinzadeh Sahafi 3 , Saeid Tamadoni 4 , Abdolreza Nabinejad 5 , Vahid Noaman 6
1 - - گروه بیولوژی دریا، دانشکده علوم و فنون دریایی ،دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران.ایران
2 - - گروه بیولوژی دریا، دانشکده علوم و فنون دریایی ،دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران.ایران
3 - موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات ،آموزش و ترویج کشاورزی، تهران. ایران.
4 - موسسه تحقیقات علوم شیلاتی کشور، پژوهشکده اکولوژی خلیج فارس و دریای عمان، سازمان تحقیقات ،آموزش و ترویج کشاورزی، بندرعباس. ایران
5 - بخش تحقیقات دامپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان اصفهان، سازمان تحقیقات آموزش و ترویج کشاورزی، اصفهان. ایران
6 - بخش تحقیقات دامپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان اصفهان، سازمان تحقیقات آموزش و ترویج کشاورزی، اصفهان. ایران.
Keywords: Persian Gulf, bivalvia, 16S rRNA, phylogeny,
Abstract :
Inroduction & Objective:Bivalvia are one of the least studied orders in the Persian Gulf .A survey and molecular analysis was conducted to determine bivalvia species diversity in the Persian Gulf.Phylogenetic relationships among all described species of the bivalvia ,were examined with nucleotide sequence data from portions of mitochondrial gene 16srRNA We provide 16s barcode sequences of commertial bivalvia of Persian Gulf ,Iran.Industrial activities , ecologic consideration , and goals of the bivalvia mollecular Identification campaign make it crucial that species of the south costal be identified. The reconstruction of evolut phylogeny of these species are crucial for revealing stock identity that can be used for the management of fisheries industries in Iran. Mitochondrial DNA sequences were used to reconstruct the phylogeny of the bivalvia species .Material and Methods:: For the purpose , DNA was extracted using Isoamyl alcohl phenol chloroform method. The evolutionary relationships among 16 species of the bivalvia were examined using 550 bp of mitochondrial DNA from the 16s rRNA gene.Results: Finally the cladograms were compared and the resulted phylogenetic trees confirmed that the Iran ′s species origin is Indo west pacific species .some of Iran′s species , which were grouped with the other bivalvia taxa seem to always form a sister clade with Indo-west pacific species with strong bootstrap .Conclusion: The result completely agrees with the previously defined species using morphological characters. However , we still lack any comprehensive and clear understanding of phylogenetic relationship in this group.
