The effects Of deferoxamine on angiogenesis in chick embryo chorioallantoic membrane

Subject Areas :

Atena Dashtizade ¹ (دانشگاه آزاد اسلامی واحد مشهد، گروه زیست شناسی، کارشناسی ارشد سلولی تکوین جانوری، مشهد. ایران.)
Javad Baharara ² (دانشگاه آزاد اسلامی واحد مشهد، گروه زیست شناسی، دانشیار بیولوژی تکوین جانوری، مشهد. ایران)
Saeedeh Zafar Balanezhad ³ (دانشگاه آزاد اسلامی واحد مشهد، گروه زیست شناسی، مربی بیولوژی تکوین جانوری، مشهد. ایران)
Khadijeh Shahrokh Abadi ⁴ (دانشگاه آزاد اسلامی واحد مشهد، گروه زیست شناسی، استادیار ژنتیک، مشهد. ایران.)

Received: 2015-05-09 Accepted : 2018-08-04 Published : 2013-07-23

Keywords: angiogenesis, Chorioallantoic Membrane, Deferoxamine,

Abstract :

Inroduction and Objective: Deferoxamine (DFO), an iron chelator, used to treat diseases of iron poisoning and affects process of angiogenesis In the present research, the effect of deferoxamine on angiogenesis of chick embryo chorioallantoic membrane was assessed.Materials and Methods: 40 fertilized eggs were divided into 4 equal groups: control group and experimental control 1,  experimental 2 and 3 treated with 10 and 100µm DFO, respectively. On day 8 of incubation, a gelatin sponge was placed on chorioallantoic membrane (CAM) and was soaked with 10 µl of DFO (10 μm) for group 2 and for group 3 with 10 µl of DFO (100 μm). On day 12, number and length of vessels in all samples was measured by Image J software. The obtained data was statistically analyzed by means of SPSS software (v.19), ANOVA and t test PResults: Mean number and length of vessels in the control and experimental control 1 cases didn’t show any significant differences (p > 0.05). Comparison between mean number and length of vessels in the control and group 1 showed a significant decrease (p 0.05).Conclusion: Deferoxamine cause inhibition of angiogenesis in chick embryo chorioallantoic membrane.