Identification of dehydration-responsive element binding transcription factor gene in Bromus japonicus under salinity stress

Subject Areas : Weed Research Journal

mahboobeh basiri ¹ , seyed mohsen mosavi nik ² , seyed kazem sabagh ³ , davood naderi ⁴ , Asiyeh Siahmarguee ⁵ , Khodadad Mostafavi ⁶

1 - gaduated
2 - Dept. of Agronomy and Plant Breeding Faculty of Agriculture Zabol University
3 - Assoc. Prof., Dept. of Biology, Faculty of Science, Yazd University, Yazd, Iran
4 - Laboratory Expert, Institute of Plant Biotechnology, University of Zabol, Zabol, Iran
5 - Assist. Prof., Dept. of Agronomy, Faculty of Agriculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
6 - Associate Professor, Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran

Received: 2017-03-05 Accepted : 2017-03-10 Published : 2019-08-23

Keywords: AP2 domain, sequence of the gene, transcription factors, Resistance of salinity,

Abstract :

Invasive plant species that are characterized as weed, plants are resistant to environmental hard conditions that this feature can be used to the genetic alternation and transformation to resistant crops. The aim of this study was identification of gene involved in tolerance to abiotic environmental stresses in the Bromus japonicus called DREB2. in this subject, salinity stress was applied via sodium chloride, Sequencing and Identification of stress resistance gene in this plant and then sequence obtained was examined. Sequence Analysis and Comparing sequence obtained with sequences this gene in other plants was performed with softwares such as BLAST, ExPasy, ELM, Clustal W, Meg Align. The result was showed that the amplified fragment belonged to part of salinity-responsive element binding-factor gene in this plant. The comparison DREB2 gene sequence with other sequences this gen in the other plant species was confirm the Bromus japonicus and Aegilops tauschii have the most similarity with 89.9% the highest likeness (the lowest genetic distance). Bioinformatics analysis of the amino acid sequence of the translated nucleotide sequence of the gene DREB2 in the Bromus japonicus through software CDD search showed that AP2 Domain area is in this sequence, These sequence was submitted to GenBank, Its accession code is KP406596.