The effect of microcystin toxin from algae microcystis on human liver carcinoma HepG2 cell line by using Real-TimePCR
Subject Areas : cellular , molecular
samaneh
mohammadkhani
1
(department biology,Islamic Azad univercity,Tehran Bruch)
roudabeh
behzadi andouhjerdi
2
(ISLAMIC AZAD UNIVERSITY-CENTER UNIT)
فاطمه
نوری
3
(استادیار، دانشگاه آزاد اسلامی، واحد تهران مرکز، تهران، ایران)
Keywords: cancer, microcystin, HepG2,
Abstract :
Pioneering and Objective: Microsystemin is a brain heptapide that is known as the most abundant cyanotoxin produced in the world's most extensive distribution, and is the most commonly produced hepatotoxin produced by cyanobacteria. This complication affects vertebrate cells, and thus the cyanotoxins are limited to limbs, which, like the liver, express and transport anionic organic carriers on their cells. In this study, the expression of BAX and BCL2 genes in the HepG2 cell line under the influence of Microsystemin was investigated using Real-TimePCR, so that it can be studied further by studying the mechanism and its effect as an appropriate target for treatment. Liver cancer.Methods: At first, HepG2 Cell Cell Passage was performed, then MTTassay test was performed. Next, RNA extraction and CDNA extraction were used and the RNA sample was purified from the nanotypic spectrophotometer, then the specific and suitable primers for the desired gene were designed And synthesized and using the Real-Time PCR, the expression of Bax and BCL2 genes was measuredResults: The BAX gene was expanded as a pro-apoptosis, and the more hepG2 was exposed to venom, the higher the expression of BAX, and the expression of BCL2 decreased, and in this case the cell went to apoptosis
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