Molecular investigation of the adhesin gene sfaC in isolates of Escherichia coli (E.coli) tissue of patients with colorectal cancer and inflammatory bowel disease large Iranian population
Subject Areas : microbiology
الیکا حاجی آقا ابراهیم
1
(دانشجوی کارشناسی ارشد)
شهلا محمد گنجی
2
(عضو هیات علمی دانشگاه ازاد اسلامی قم)
Mohsen Zargar
3
(عضو هیات علمی- دانشگاه آزاد اسلامی واحد قم)
Keywords: Escherichia coli(E.coli), Adhesin gene sfaC, Colorectal cancer (CRC), Inflammatory bowel disease (IBD),
Abstract :
Colorectal cancer after lung cancer, stomach and liver cancer is fourth most common cancer in Iran and worldwide. Studies have shown that patients with inflammation bowel disease (IBD) in large intestine are at increased risk of CRC. Various factors play a role in colorectal cancer that bacteria are one of these factors. E. coli is a member of the Microbiota of human intestinal tract in the colon. Also, the ability of binding to host cells is the most basic step in successful colonization in microbial pathogens. Some strains of E. coli has a number of fimbriae and adhesion, such as P fimbriae, S fimbriae, and adhesion of bacteria afimbrial which gives the ability to connect to the host cells. This study was performed to investigate the sfaC adhesin gene in E.coli isolated from intestinal tissue biopsies of patients with inflammation bowel disease and colorectal cancer. 38 biopsies were obtained from intestinal tissue and bacteria were isolated and identified using microbial and biochemical methods. After DNA extraction, strains for adhesin gene sfaC were evaluated using polymerase chain reaction (PCR).Molecular studies showed that the number of positive samples for this gene (sfaC) in normal individuals and in patients with inflammatory bowel disease and patients with colorectal cancer, were 42.8%, 62.5% and 60% of, respectively. The results of this study shows the relationship between the adhesin gene and colorectal cancer.
