The study of the effects and side effects of methyldopa drug on changes in male rat kidney tissue

Subject Areas : zoology

Mehdi  Ahmadifar ¹ (Royan Institute)
سید مهدی  کلانتر ² (هیئت علمی)
ناصر  کلهر ³ (مدرس دانشگاه آزاد قم)
سپیده  جعفرزاده راستین ⁴ (دانشجو/ دانشگاه شهید صدوقی یزد)
فاطمه  تقوایی ⁵ (دانشجو/ دانشگاه شهید صدوقی یزد)
مهدی  لطفی پناه ⁶ (پژوهشگر/ پژوهشگاه رویان)

Received: 2016-04-04 Accepted : 2016-05-18 Published : 2016-08-22

Keywords: methyldopa, kidney tissue, Blood pressure, Rat,

Abstract :

Introduction: Increased kidney disease in human societies is now one of the urologists concerns. Many of these kidney disorders can be caused by various drugs including blood pressure lowering drugs.Methods: In this experimental study, 40 male wistar rats were used. The rats were divided into 4 groups of 10 including control, first, second and third experimental groups which received respectively water, 0.03, 0.06 and 0.08 mg/ kg methyldopa drug via gavage. After 1 month, these40 mice were anesthetized and their kidneys were removed.After preparing microscopic sections, they were stained using H & E staining and stained samples were observed with a light microscope. Then images of desired samples were prepared using a light microscope equipped with camera.Results: the obtained results shows that the consumption of high doses methyldopa drugs lead to disturbance in kidney function and related enzymatic processes. Moreover it can cause remarkable changes in different kidney layers.Conclusion:regarding obtained results, prescribing methyldopa drug should be done more carefully.