Gill Histopathological Changes of Common Carp (Cyprinus carpio) During Exposure to Sub-Lethal Concentrations of Copper Oxide Nanoparticles

Subject Areas : Environmental pollutions (water, soil and air)

hasan sahraei ¹ , Seyyed Abbas Hoseini ² , Seyyed Aliakbar Hedayati ³ , Rasoul Ghorbani ⁴

1 - P.hd. Student, Dept. of Fisheries, Faculty of Natural Resources, Gonbad kavous University, Gonbad kavous, Iran
2 - Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources
3 - Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources
4 - Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources

Received: 2015-07-29 Accepted : 2015-09-13 Published : 2018-03-21

Keywords: Common Carp, Copper oxide nanoparticles, hyperplasia, Histopathology,

Abstract :

Background and Objective:At the present century, using of copper Nano-particles has increased within the environment. In this regard influx of Nano-materials to the environment may have harmful impact on living organisms, including fish. The aim of this study was to examine histological lesions of cooper nanoparticles on the gill tissue of common carp. Method: In order to investigate the effects of histopathological changes of Nano copper oxide on the gill of carp, 60 pieces of young common carp with an average weight of 42± 2 gr were divided in 3 groups with three replicates for exposure group of Nano copper oxide (10, 40, 80, mg/l of copper oxide) and a control group of fish for 6 weeks. Findings: Histopathology of Gill tissue showed that the copper nanoparticles have such injuries as cell swelling, Aourtism , edema, secondary connecting adjacent blades, shortening of the secondary blade, and hyperplasia. Gill histopathological result showed that at high concentrations (80 ppm), lesions were mostly detachment, hyperplasia and sticking attach Lamela, versus harm in low concentrations (10 and 40 ppm) to form  Aurism, inflation squamous cell and the shortening of the secondary blades. Discussion and Conclusion Due to changes of gill tissue in the common carp at various concentrations of copper nanoparticles compared with the control group, it can be concluded that copper nanoparticles can have significant effects on fish gill tissue. Therefore it can be used as a simple and useful tool to assess the impact of these new born contaminants on fish and also human environmental.

References:

1. Salarijoo, H,. Kalbasi, M,. Johari,. A. 2012. Influence of water on acute toxicity of colloidal nanoparticles in rainbow trout (Oncorhynchus mykiss). Journal of Health and Environment, Vol 5, No 1, Pp. 121-132(In Persian).


2. Scown, T. M., Santos, E. M., Johnston, B. D., Gaiser, B. 2010. Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout.Toxicol. Sci.115(2): 521-534.


3. Arora, S., Jain, J., Rajwade, J. M., Paknikar, K. M. 2008. Cellular responses induced by silver nanoparticles: in vitro studies, Toxicol. Let.179(2): 93–100.


4. Al-Bairuty, G. A., Shaw, B. J., Handy, R. D., Henry, T. B. 2013. Histopathological effects of waterborne copper nanoparticles and copper sulphate on the organs of rainbow trout (Oncorhynchusmykiss). Aquatic toxicology. 126, 104-115.


5. Li, B., Hwang, J.Y., Drelich, J., Popko, D., Bagley, S. 2010. Physical,chemical and antimicrobial characterization of copper-bearing material.Jom Journal of the Minerals,Metals and Materials Society. 62: 80-95.


6. Gaetke, L. M., Chow, C. K. 2003. Copper toxicity, oxidative stress, and anti oxidant nutrients Toxicology. 189:147-154.


7. Ramsden, C. S. 2009. Dietary exposure to titanium dioxide nanoparticles in rainbow trout (Oncorhynchus mykiss) no effect on growth, but subtle biochemical disturbances in the brain. Ecotoxicolog. 18: 939-951.


8. Hao, L., Wang, Z,. Xing, B. 2009. Effect of sub-acute exposure to TiO2 nanoparticles on oxidative stress and histopathological changes in Juvenile Carp (Cyprinus carpio), Journal of Environmental Sciences.21:1459–1466.


9. Zhao, J., Wang, Z., Liu, X., Xie, X., Zhang, K., Xing, B. 2011. Distribution of CuO nanoparticles in juvenile carp (Cyprinus carpio) and their potential toxicity. Journal of hazardous materials, 197, 304-310.


10. Martoja, R. 1967. Martoja–Pierson, M., Initiation Aux Techniques de l histology animale. Masson etCie, Paris.345 p.


11. Gernhofer, M., Pawert, M., Schramm, M., Muller, E., Triebskorn, R. 2001. Ultra structural biomarkers as tools to characterize the health status of fish in contaminated streams. Journal of Aquatic Ecosystem Stress and Recovery.8: 241-260.


12. Schwaiger, J., Fent, K., Stecher, H., Ferling, H., Negele, R. D. 1996. Effects of sub lethal concentrations of triphenyltinacetate on rainbow trout (Oncorhynchusmykiss).Archives of Environmental Contamination and Toxicology. 30(3), 327-334.


13. Alazemi, B. M., Lewis, J.W., Andrews, E. B. 1996. Gill damage in the freshwater fish Gnathonemuspetersi(family: Mormyridae) exposed to selected pollutants: an ultrastructural study. Environmental Technology.17 (3): 225–238.


14. Lin, Y.M., Chen, C.N., Lee, T. H. 2003. The expression of gill Na, K-ATPase in milkfish, Chanos chanos, acclimated to seawater, brakishwater and freshwater. Comp Biochem Physiol A Mol Integr Physion. 135(3): 489-497.


15. Evans, D. H. 1993. Osmotic and ionic regulation, In: The physiology of fishes, edited by D.H. Evans, Boca Raton, FL: CRC, 315–341.


16. Rostami, M,. Soltani, M. 2009. Study of tissue effects of chronic copper sulfate on some organs of common carp, Journal of Veterinary Research, Vol. 64, No. 3, Pp 193-198.(In Persian).


17. Genten, F., Terwinghe, E., Danguy, A. 2009. Atlas of histology. Published by Science Publishers, Enfield, NH, USA.


18. Evans, D. H. Piermarini PM, Choe KP. 2005. The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation and excretion of nitrogenous waste. Physiol Rev. 85: 97- 177.


19. Schlenk, D. and Benson, W. H. 2001. Target organ toxicity in marine and fresh water teleosts. Taylor and Fransis, PP. 1-90.


20. Oliveira-Filho, E.C., Muniz, D.H.F., and Ferreira, M.F.N. 2010. Cesar Koppe Grisolia evaluation of acute toxicity,cytotoxicity and genotoxicity of a nickel mining waste to(Oreochromis niloticus). B Environ Contam Tox. 85:467-471.


21. Cengiz, E.I., Unlu, E. 2006.  Sublethal effects of commercial deltamethrin on the structure of the gill, liver and gut tissues of mosquitofish Gambusia affinis, A microscopic study.Environmental Toxicology and Pharmacology.21(3): 246-253.


22. Chen, J., Dong, X., Xin, Y., & Zhao, M. 2011.  Effects of titanium dioxide nano-particles on growth and some histological parameters of zebrafish (Danio rerio) after a long-term xposure. Aquatic Toxicology. 101(3), 493-499.‏


23. Handy, R. D., Henry, T. B., Scown, T. M., Johnston, B. D. and Tyler, C. R. Manufactured nanoparticles their uptake and effects on fish a mechanistic analysis. Ecotoxicology.17: 396–409.


24. Straus, D. 2003. The acute toxicity of copper to bluetilapia in dilutions of settled pond water.Aquaculture.219: 233-240.


25. Rostami, M,. Soltani, M,. Sasani, F. 2000.  Study of the histopathologic effects of some heavy metals (copper sulfate, zinc sulfate and mercuric chloride - cadmium sulfate) on common carp tissues. Journal of Faculty of Veterinary Medicine, University of Tehran, No. 4, Volume 55, Pp 3-1 ( In Persian).


26. Monfared, A. L., & Soltani, S. 2013.  Histological, Histometric and biochemical alterations of the            gill and kidney of rainbow trout (Oncorhynchus mykiss) exposed to silver nanoparticles. European Journal of Experimental Biology, 3(2), 391-395.


27. Patel JM, and Bahadur A. 2011. American-Eurasian J Toxicol Sci, 4(1): 01-05.


28. Wood C, Munger S, Galvez F, Hogstrand C. 1994.  Procee 2nd international conference, 11-14.


29. Heath, A. G. 1987. Water pollution and fish physiology. Boca Raton, CRC Press.


30. Martinez, C. B. R., M. Y. Nagae, C. T. B. V. Zaia & D. A. M. Zaia. 2004. Morphological and physiological acute effects of lead in the neotropical fish Prochilodus lineatus. Brazilian Journal of Biology. 64 (4): 797-807.


31. Camargo, M.M.P and Martinez, C.B.R. 2007.  Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotropical Ichthyology. 5(3): 327-336.


32. Reddy P.B. and Baghel B.S. 2010.  Archana Kushwah and Shehla Ishaque, Biochemical and  hematological studies on effect of textile Industry effluent in Muscus albinus, In; Proc. ICEM., 10 (2), 713-719.


33. Au D.W.T. 2004. The application of histo-cytopathological biomarkers in marine pollution monitoring: a review, Marine Pollution Bulletin, 48, 817-834.


34. Naji, T,. Safaeiyan, Sh,. Rostami, M,. Sabrjoo, M. 2006. Effects of zinc sulfate on gill tissue of ordinary carp. Journal of Environmental Science and Technology, No. 2: Volume 9, Pp 29 ( In Persian).


35. Hedayati, A,. Jahanbakhshi, A,. Ghaderi romazi, F. 2013. Aquatic toxicology, Gorgan, Gorgan University of Agricultural Sciences and Natural Resources. 210p (In Persian).


36. Johari, A. 2011. Application of silver nanoparticles in reducing fungal infections of eggs during incubation and their possible effects on changes in some physiological and genetic indices of rainbow trout, Ph.D., Natural Resources Faculty. Tarbiat Modares University, 148 p (In Persian).


37. Farrokhrouz, M,. Zamini, A,. Mozaffari, E. 2013. The Effects of Formalin and Copper Sulfate on Guinea Pig Gill (Rutilus kutum), Journal of Physiology and Animal Husbandry, Vol. 6, No. 4: Pp 27-35 (In Persian).