Gill Histopathological Changes of Common Carp (Cyprinus carpio) During Exposure to Sub-Lethal Concentrations of Copper Oxide Nanoparticles

Subject Areas : Environmental pollutions (water, soil and air)

hasan sahraei ¹ ( P.hd. Student, Dept. of Fisheries, Faculty of Natural Resources, Gonbad kavous University, Gonbad kavous, Iran )
Seyyed Abbas Hoseini ² ( Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources )
Seyyed Aliakbar Hedayati ³ ( Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources )
Rasoul Ghorbani ⁴ ( Associate Prof., Dept. of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources )

Received: 2015-07-29 Accepted : 2015-09-13 Published : 2018-03-21

Keywords: Common Carp, Copper oxide nanoparticles, hyperplasia, Histopathology,

Abstract :

Background and Objective:At the present century, using of copper Nano-particles has increased within the environment. In this regard influx of Nano-materials to the environment may have harmful impact on living organisms, including fish. The aim of this study was to examine histological lesions of cooper nanoparticles on the gill tissue of common carp. Method: In order to investigate the effects of histopathological changes of Nano copper oxide on the gill of carp, 60 pieces of young common carp with an average weight of 42± 2 gr were divided in 3 groups with three replicates for exposure group of Nano copper oxide (10, 40, 80, mg/l of copper oxide) and a control group of fish for 6 weeks. Findings: Histopathology of Gill tissue showed that the copper nanoparticles have such injuries as cell swelling, Aourtism , edema, secondary connecting adjacent blades, shortening of the secondary blade, and hyperplasia. Gill histopathological result showed that at high concentrations (80 ppm), lesions were mostly detachment, hyperplasia and sticking attach Lamela, versus harm in low concentrations (10 and 40 ppm) to form  Aurism, inflation squamous cell and the shortening of the secondary blades. Discussion and Conclusion Due to changes of gill tissue in the common carp at various concentrations of copper nanoparticles compared with the control group, it can be concluded that copper nanoparticles can have significant effects on fish gill tissue. Therefore it can be used as a simple and useful tool to assess the impact of these new born contaminants on fish and also human environmental.

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