Antibiotic Resistance in Isolated Negative Gram Bacteria From Intestinal Organ of Anzali Wetland Wild Common Carp

Subject Areas : environmental management

Shila Safaeian ¹ , Zahra Moghaddam ² , Hedayat Hosseiny ³ , Akbar Esmaili ⁴

1 - Assistant Professor, Faculty of Marin Biology (head of the group), Islamic Azad University, Tehran
2 - M. Sc. Student, Faculty of Marin Biology, Islamic Azad University, Tehran
3 - Assistant Professor, Member of the Science Board, Ministry of Health, Research Center, Institute of Research of Nutrition and Food Industry of the Country, Shahid Beheshti University
4 - Assistant Professor, Faculty of Marin Biology (head of the group), Islamic Azad University, Tehran

Received: 2009-11-19 Accepted : 2009-06-08 Published : 2014-02-20

Keywords: Negative Gram Bacteria, Antibiotic resistance, Wild Common Carp, Anzali Wetland,

Abstract :

Introduction: A research has been performed on antibiotics' resistance in negative gram bacteriaseparated of wild common carps intestinal organ.Material and Method: 126 negative Gram bacteria have been separated in total and resistancerate of bacteria in relation to 6 Antibiotics are measured: Ampicillin (10 microgram Am),Streptomycin (30 microgram S), Tetracycline (30 microgram T), Chloramphenicol (30 microgram C),Gentamycine (10 microgram GM), Amikacin (30 microgram AK).Results and Discussion: The results showed that negative Gram bacteria in comparison toantibiotic such as Ampicillin (93.5%), Streptomycin (51.15%), Tetracycline (46.5%) had higherresistance. So it showed that such bacteria’s resistance in comparison to Chloramphenicol (12.15%). isrelatively low and it showed loss of existence of resistance in negative Gram bacteria in comparison toGentamycine and Amikacin (0%).Surveying of the resistance rate of bacteria to some antibiotics show that 80% of separated bacteriafrom fish intestinal organ in comparison to consumption antibiotics are multi-resistance.Research shows that antibiotics consumption in animal is growing and more consumption ofantibiotics in environment could cause bioenvironmental problems such as resistance againstantibiotics. This research which was done for the first time in Anzali Wetland on Antibiotic resistancerate in negative Gram bacteria separated of wild common carp digestive system shows that negativegram system resistance against antibiotics in Anzali Wetland is high.

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