Isolation of Toluene Biodegrade Bacteria from ISFAHAN Petrochemical soil

Subject Areas : environmental management

Abbas Akhavanesepahi ¹ , Elahe Nazer ² , Bagher Yakhchaly ³ , Mohammad Reza Nazer ⁴

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Received: 2008-12-10 Accepted : 2009-06-28 Published : 2011-12-22

Keywords: Bacillus pumilus, Bioremediation, Toluene,

Abstract :

Toluene is one the common and main environmental pollutant factors which often contaminates sites. it is a result of spillages of fuels, solvents or chemicals, also it is one of the major products of petro chemical activities. Because of being highly toxic, it represents a significant environmental hazard for human and has potential toxicity, carcinogenicity, negative effects on reproduction and mortality rates of aquatic animals. This material is a mutagen as well. Microbial degradation represents the major responsible route for removing these pollutants including fungus, aerobic and anaerobic bacteria. It is important that this component degrades in a high dosage that certain species of bacteria will able to degrade it. 6Toluene-utilizing bacteria is isolated from petrochemical of Isfahan soils .Amongst them one strain tolerant toluene is highly concentrated. They were isolated when bacteria exposed on vapor pressure of toluene (1-100%). They used toluene as sole sources of carbon and energy. This strain was identified by biochemical characteristic and 16S rRNA sequencing as Bacillus. Also amount of deletion of toluene were detected by gas chromatography (GC) during growth of the bacteria. The investigated isolates are considered to have a good potential for application in remediation of toluene contaminated environments and industrial wastewater and soils.