Isolation and Evaluation of Meso-philic, Thermo-philic and SRBs Bacteria and Fungi Effective on the Effluent Wastewater in the Moulding Industrials

Subject Areas : environmental management

Mohammad Hossein Habibollahi ¹ , Mohammad Kargar ² , Abbasali Damangir ³ , Sara Nabizadeh ⁴

1 - PhD Student, Microbiological Science, Karaj Islamic Azad University.
2 - Assistant Professor, Faculty of Microbiological Science, Jahrom Islamic Azad University
3 - PhD Student, Agricultural Science, Faculty of Research and Science, Tehran Islamic Azad University
4 - Msc. Farsi Language and Literature

Received: 2010-08-11 Accepted : 2011-01-05 Published : 2014-05-22

Keywords: Bio-surfactant, Wastewater, Aerobes and Anaerobes bacteria, Moulding industrial,

Abstract :

Introduction: Nowadays due to attention to progress of environmental laws and the importance of the wastewater purification, treatment and degradation of it have been considered as one of the important subjects. The purpose of this research is isolation and evaluation of aerobes and anaerobes microorganisms effective on oil wastewater. Materials and Methods: The specimens of this research were collected from effluent wastewater containing oil compounds of moulding unit of Sarcheshmeh Copper Complex. Mesophilic, Thermophilic and SRBs bacteria and fungus were isolated in special media. Then all of the colonies were evaluated for purpose of bio-surfactant production by emulsification tests and surface tension reduction by Tensio-meter in M.S.S medium. At last the effects of colonies with the emulsification power of 2.5–4 and surface tension reduction up to 9mN/m on wastewater structure were evaluated by HPLC chromatography. Result and Discussion: Only 15 Mesophilic bacteria of 104 isolated microorganisms have shown the most effect on the wastewater structure. Thermophilic and SRBs bacteria and fungus had insignificant effects on wastewater structure. With due results of this research, omitting heavy and organic compounds from wastewater aerobe and Mesophilic bacteria should be considered more effectively.

References:

1. Kadarwati, S., Herlina, L., 2008. Effect of biosurfactant produced by bacillus in only wastewater degradation. Lemigas scientific contributions, Vol. 31, No. 3, pp. 40-46


2. Shaban, AM., Kamal, MM., Kenawy, N., El Manakhly, H., 2009. Role of interior structure of agro and non-agro materials for industrial wastewater treatment. Jounal of Applied Sciences Research, Vol. 5, No. 8, pp. 978-985


3. El-Bestawy, E., Hussein, H., Baghdadi, HH., El-Saka, MF., 2005. Comparison between biological and chemical treatment of wastewater containing nitrogen and phosphorus. J and Microbiol Biotechnol,Vol. 32, pp. 195-203


4. Izanloo, H., Mesdaghinia, A., Nabizadeh, R., Naddafi, K., Nasseri, S., Mahvi, AH., Nazmara, S., 2007. The treatment of wastewater containing crude oil with aerated submerged fixed-film reactor. Pakistan Journal of Biological Sciences, Vol. 10, No. 17, pp. 2905-2909


5. Helmy, Q., Kardena, E., Nurachman, Z., Wisjnuprapto., 2010. Application of biosurfactant produced by Azotobacter vinelandii AVO1 for enhanced oil recovery and biodegradation of oil sludge. International Journal of Civil & Environmental Engineering IJCEE, Vol. 10, No. 1, pp. 7-14


6. Rossmoore, HW., Rossmoore, LA., 1993. MIC in metalworking processes and hydraulic systems. A practical manual on Microbiologically Influenced Corrosion Engineers (NACE), Chapter, 5, pp. 31-40


7. کارگر، محمد و همکاران، «شناسایی باکتری های مولد بیوسورفکتانت و کاربرد آنها در حذف آلاینده های نفتی»، فصلنامه علوم و تکنولوژی محیط زیست، تابستان 1385، دوره هشتم، شماره 2، صفحات 109تا 118


8. Tabatabaee, A., Mazaheri, M., Noohi, AA., Sajadian, VA., 2005. Isolation of biosurfactant producing bacteria from oil reservoirs. Iranian J Env Health Sci Eng, Vol. 2, No. 1, pp.6-12


9. Rossmoore, LA., Rossmoore, HW., 1994. Metalworking fluid microbiology. Chapter, 9., pp. 247-271


10. Chipasa, KB., 2001. Limits of physicochemical treatment of wastewater in the vegetable oil refining industry. Polish Journal of Environmental Studies, Vol. 10, No. 3, pp. 141-147


11. Matsui, T., Miura, A., Liyama, T., Shinzato, N., Matsuda, H., Furuhashi, K., 2004. Effect of fatty oil dispersion on oil-containing wastewater treatment. Elsevier B.V, pp. 1-4.