Isolation and molecular identification of the bacteria involved in removing phosphate from industrial wastewater

Subject Areas : Environmental pollutions (water, soil and air)

Seyed Hossein Hosseini ¹ , Maryam Tabibi ² , Hamidreza Pordeli ³ , Reza Najafpour ⁴ , Fatemeh Karimi ⁵ , Sajjad Yazdansetad ⁶

1 - MSc of Microbiology, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Semnan, Iran.
2 - PhD Candidate of Microbiology, Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
3 - Associate Professor of Mycology, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Gorgan, Iran.
4 - PhD Candidate of Microbiology, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Qom Branch, Qom, Iran.
5 - PhD Candidate of Microbiology, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Qom Branch, Qom, Iran.
6 - PhD Candidate of Microbiology, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran.* (Corresponding Author)

Received: 2015-03-31 Accepted : 2015-11-17 Published : 2017-08-23

Keywords: Phosphate-removing bacteria, Bioremediation, Wastewater, Contaminant,

Abstract :

Background and Objective: Phosphate is one of the most important contaminants entering recipient waters (rivers, lakes, and seas) by wastewater disposal and causative agent of eutrophication due to the enrichment of aquatic ecosystems. In bioremediation process, the phosphate-removing bacteria accumulate polyphosphate intracellularly and take it away from the environment. The objective of this study was to isolate and identify the bacteria which remove phosphate from industrial wastewater. Method: In this study, phosphate-removing bacteria were isolated from wastewaters of Aq Qlala industrial park of Golestan province. The isolates were identified based on the creation of clear zone in the bacterial lawn, leading to phosphate removal on the specific agar plate Seperb. Finally, the isolates were identified by macroscopical, microscopical, biochemical, and molecular methods. Findings: In total, 3 out of 30 isolates had high ability in phosphate removal regarding their large clear zone on agar. Molecular identification of isolates by 16S rDNA typing method indicated that the isolates belong to the genera Brevundimonas, Ochrobactrum, Exiguobacterium. Conclusion: Variance analysis using SAS 9.2 software indicated a significant difference in phosphate removal by the isolates. The obtained results demonstrated that the isolates are highly efficient in phosphate removing from wastewater and they are suitable candidates for bioremediation along with other methods.

References:

1-      Usharani, K. and Lakshman aperumalsamy, P., 2010. Bio-treatment of phosphate from synthetic wastewater using Pseudomonas sp YLW-7. Journal of Applied Sciences and Environmental Management, Vol. 14, No. 2, pp. 75-80.

2-      DebRoy, S., S. Das., S. Ghosh., S. Banerjee and D. Chatterjee et al., 2012. Isolation of nitrate and phosphate removing bacteria from various environmental sites. OnLine Journal of Biological Sciences, Vol. 12, No. 2, pp. 62-71.

3-      Van Loosdrecht, M.C.M., C.M. Hooijmans., D. Brdjanovic., J.J. Heijnen., 1997. Biological phosphate removal processes. Applied Microbiology and Biotechnology, Vol. 48, No. 3, pp. 289-296.

4-      Taheriyoun, M., Karamouz, M., and Baghvand, A., 2010. Development of an entropy based fuzzy eutrophication index for reservoir water quality evaluation. Journal of Environmental Health Science and Engineering., Vol. 7, No. 1, pp. 1-14.

5-      Cech, J.S., Hartman, P., 1993. Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems. Water Research., Vol. 27, No. 7, pp. 1219-1225.

6-      Kulaev, I.S., Vagabov, V.M., Kulakovskaya, T.V., 2004. The biochemistry of inorganic polyphosphates. 2th ed., New York, John Wiley & Sons.

7-      Pasayeva, P., Gezqin, Y., Pekin, G., Eltem, R., 2011. Phosphate uptake performance of bacteria isolated from a full-scale Izmir municipal wastewater treatment plant. Environmental Technology., Vol. 32, No. 5-6, pp. 543-549.

8-      Benammar, L., Menasria, T., Ayachi, A., Benounis, M., 2015. Phosphate removal using aerobic bacterial consortium and pure cultures isolated from activated sludge. Process Safety and Environmental Protection., Vol. 95, No. 1, pp. 237-246.

9-      Eaton, A.D., Clesceri, L.S., Rice, E.W., Greenberg, A.E., editors., 2005. Standard methods for the examination of water and wastewater. 21th ed. Washington, DC: American Public Health Association (APHA)-American Water Works Association (AWWA)-Water Environment Federation (WEF). 1368 p.

10-  Teimouri, M., Korori, S.A.A., Matinizadeh, M., and Khoshnevis, M., 2005. Isolation and identification of phosphate solubilizing bacteria from Vaz forest soil. Pajouhesh & Sazandegi., Vol. 17, No. 65, pp. 57-64. [In Persian].

11-  John, G.H., Noel, R.K., Peter, H.S., James, T.S., Stanley, T.W., 1997. Bergey´s Manual of Determinative Bacteriology (In Russian). 9th ed. Moscow: Mir Publishers 2.

12-  Sambrook, J., Russell, D., and Irwa, et al., 2001. Molecular Cloning, a Laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press.

13-  Krishnaswamy, U., Muthusamy, M., and Perumalsamy, L., 2009. Studies on the Efficiency of the Removal of Phosphate Using Bacterial Consortium for the Biotreatment of Phosphate Wastewater. European Journal of Applied Sciences., Vol. 1, No. 1, pp. 6-15.

14-  Hupfer, M., Gloess, S., Grossart, HP., 2007. Polyphosphate-accumulating microorganisms in aquatic sediments. Aquatic Microbial Ecology., Vol. 47, No. 3, pp. 299-311.

15-  Hupfer, M., Glöss, S., Schmieder, P and Grossart, HP., 2008. Methods for Detection and Quantification of Polyphosphate and Polyphosphate Accumulating Microorganisms in Aquatic Sediments. International Review of Hydrobiology., Vol. 93, No. 1, pp. 1-30.

16-  Brdjanovic, D., Hooijmans C.M., Van Loosdrecht, M.C.M., Alaerts, G.J., and Heijnen, J.J., 1996. The dynamic effects of potassium limitation on biological phosphorus removal. Water Research., Vol.  30, No. 10, pp. 2323-2328.

17-  Park, J., Bolan, N., Megharaj, M., Naidu, R., 2010. Isolation of Phosphate-Solubilizing Bacteria and Characterization of Their Effects on Lead Immobilization. Pedologist, Vol.  53, No. 1, pp: 67-75.

18-  Rodrigues, D.F., Goris, J., Vishnivetskaya, T., Gilichinsky, D., Thomashow, M.F., Tiedje, JM., 2006. Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov. Extremophiles, Vol. 10, No. 4, pp. 286-294.

19-  Sidat, M., Bux, F and Kasan, H.C., 1999. Polyphosphate accumulation by bacteria isolated from activated sludge. Water SA., Vol. 25, No. 2, pp. 175-179.