The Investigation of Effect of Soil Moisture and Temperature on Crude Oil Bioremediation by Pseudomonas Putida
Subject Areas :
soil pollution
Akbar Ghavidel
1
(
Assistant Prof., Department of Soil Science and Engineering, University of Mohaghegh Ardabili, Ardabil, Iran
)
Sumayyah Naji rad
2
(
Assistant Prof., Department of Environmental Science, Islamic Azad University, Ardabil Branch, Ardabil, Iran * (Corresponding Author)
)
Hossein Ali Alikhani
3
(
Professor, Department of Soil Science and Engineering, University of Tehran, Tehran, Iran.
)
Received: 2016-09-28
Accepted : 2017-05-16
Published : 2021-03-21
Keywords:
Pseudomonas Putida,
Environmental Conditions,
Bioremediation,
Crude Oil Contamination,
Abstract :
Background and Objective: Bioremediation is an approach that exploits the ability of microorganisms to increase the rate and extent of degradation of pollutants and thereby removing pollutants from the environment. The moisture content and temperature are of the main environmental factors affecting growth and activity of microorganisms and accordingly affecting the efficiency of organic pollutant biodegradation.Method: To study the effect of these two factors a factorial experiment was carried out as completely randomized design with three replications. The factors were moisture in three levels (30%, 55% and 80% of Field Capacity), temperature in three levels (25, 30 and 35 degrees of Celsius) and inoculation with bacteria in two levels (with and without inoculation by Pseudomonas putida) which were triplicated.Findings: The results showed that highest biodegradation rate was observed in the treatment with the moisture content of 55% F.C, temperature of 30 degrees of Celsius and inoculation with Pseudomonas putida which was 92.8% and the lowest biodegradation rate was observed in the treatment with the moisture of 30% F.C, temperature of 30 degrees of Celsius and without inoculation which was 42.3%.Discussion and Conclusion: These results shows that the optimization of the environmental conditions in bioremediation process may lead to 50.5% increase in the efficiency of removal.
References:
Guarino, C., Spada, V., and Sciarrillo, R., 2017. Assessment of three approaches of bioremediation (Natural Attenuation, Landfarming and Bioagumentation – Assistited Landfarming) for a petroleum hydrocarbons contaminated soil. Chemosphere 170:10-16.
Mirsal Ibrahim, A., 2008. Soil pollution: origin, monitoring and remediation, 1st Ed., Springer, Germany. 312 p.
Sajna, K.V., Sukumaran, R.K., Gottumukkala, L.D., and Pandey, A., 2015. Crude oil biodegradation aided by biosurfactants from Pseudozyma sp. NII 08165 or its culture broth. Bioresource Technology 191:133-139.
Zhu, XAD., Venosa, MT., and Suidan, M., 2004. Literature review on the use of commercial bioremediation agents for cleanup of oil-contaminated estuarine environments. EPA/600/R-04/075.
Yang, S Z., Jin, HJ., Wei, Z., He, RX., Ji, YJ., Li, XM., and Yu, SP., 2009. Bioremediation of oil spills in cold environments: A review. Pedosphere. 19(3): 371–381.
Minoui, S., Minai-Tehrani, D., Eslami, G., and Sobhani-Damavandifar, Z., 2009. Change in cytochromes content of Pseudomonas sp. in the medium containing petroleum. Australian Journal of Basic and Applied Sciences, 3(3):1512-1516.
Tekorienė, R., 2008. Distribution of the genus Pseudomonas bacteria in oil-polluted soil, water, polymeric materials, plant remnants and food products. Ekol. 54(3):143–148.
Taoufik, J., Zeroual, Y., Moutaouakkil, A., Moussaid, S., Dzairi, FZ., Talbi, M., Hammoumi, A., Belghmi, K., Lee, K., Loutfi, M., and Blaghen, M., 2004. Aromatic hydrocarbons removal by immobilized bacteria (Pseudomonas sp., Staphylococcus sp.) in fluidized bed bioreactor. Annals of Microbiology, 54:189-200.
Guieysse, B., Viklund, G., Toes, AC., and Mattiasson, B., 2004. Combined UV-biological degradation of PAHs. Chemosphere, 55(11):1493-1499.
Nnamchi, CI., Obeta, JAN., and Ezeogu, LI., 2006. Isolation and characterization of some poly aromatic hydrocarbon degrading bacteria from Nsukka soils in Nigeria. Int. J. Environ. Sci. Tech. 3(2): 181-190.
Bayat, Z., Hassanshahian, M., Hesni, M.A., 2015. Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf. Marine Pollution Bulletin 101, 85-91.
Di Martino, C., López, N.I., and Raiger Iustman, L.J., 2012. Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation. International Biodeterioration & Biodegradation 67:15-20.
Ma, K.-Y., Sun, M.-Y., Dong, W., He, C.-Q., Chen, F.-L., and Ma, Y.-L., 2016. Effects of nutrition optimization strategy on rhamnolipid production in a Pseudomonas aeruginosa strain DN1 for bioremediation of crude oil. Biocatalysis and Agricultural Biotechnology 6:144-151.
Shim, H., Hwang, B., Lee, SS., and Kong, SH., 2005. Kinetics of BTEX biodegradation by a coculture of Pseudomonas putida and Pseudomonas fluorescens under hypoxic conditions. Biodegradation, 16: 319–327.
Walkey, A., and Black, IA., 1934. An Examination of the Degtjareff Method for Determining Soil Organic Matter and a Proposed Modification of the Chromic Acid Titration Method. Soil Science, 37: 29-38.
Olsen, SR., and Sommers, LE., 1982. Phosphorus. In: Page AL. (ed), Methods of Soil Analysis, Agron. No. 9, Part 2: Chemical and microbiological properties, 2nd ed., Am. Soc. Agron., Madison, pp: 403- 430.
Page, AL., Miller, RH., and Keeney, DR., 1982. Method of soil analysis (part 2: chemical & microbiological properties). American society of Agronomy, Madison, 1121 p.
Atagana, HI., Haynes, RJ., and Wallis, FM., 2003. Optimization of soil physical and chemical conditions for the bioremediation of creosote-contaminated soil. Biodegradation, 14(4): 297-307.
USEPA. 2001. Guideline for the bioremediation of marine shorelines and fresh water wetland. Office of research and development, US Environmental Protection Agency.
Eaton, AD., and Franson, MAH., 2005. Standard Methods for the Examination of Water & Wastewater: American Public Health Association. Washington DC. 1020 p.
Asrari, E., Tavallali, H., and Hagshenas, M., 2010. Removal of Zn (II) and Pb(II) ions Using Rice Husk in Food Industrial Wastewater, Journal of Applied Sciences and Environmental Management, 14(4): 159–162.
Safahie, A., Mojudi, F., and Zolgharnain, H., 2011. Evaluation and Comparision of the Ability of Pseudomonas strain indigenous to Khormousa in removal of Aromatic Cmpounds. Journal of Environmental Studies, 58:149-158. (In Persian).
Naji Rad, S., Alikhani, HA., Savaghebi, GR., Saleh-Rastin, N., and Farahani, M., 2006. Bioremediaton of Soil Contaminated to Petroleum Hydrocarbons. Master of Science Thesis, University of Tehran. (In Persian)
Das, k., and Mukherjee, K., 2007. Crude petroleum-oil biodegradation eyciency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India. Bioresource Technology 98: 1339-1345.
Kucerova, R., 2006. Application of pseudomonas putida and rhodococcus sp. By biodegradation of PAH(s), PCB(s) and nel soil samples from the hazardous waste dump in pozďátky (czech republic). Rud. Geol. Naft. 18:97-101.
Liu, B., Ju, M., Liu, J., Wu, W., Li, X., 2016. Isolation, identification, and crude oil degradation characteristics of a high-temperature, hydrocarbon-degrading strain. Marine Pollution Bulletin 106:301-307.
Bushnaf, K.M., Puricelli, S., Saponaro, S., Werner, D., 2011. Effect of biochar on the fate of volatile petroleum hydrocarbons in an aerobic sandy soil. Journal of Contaminant Hydrology 126:208-215.
Varjani, S.J., 2017. Microbial degradation of petroleum hydrocarbons. Bioresource Technology 223:277-286.
Yuan, SY., Wei, SH., and Chang, BV., 2000. Biodegradation of polycyclic aromatic hydrocarbons by a mixed culture. Chemosphere 41:1463–1468.
Nwinyi, O.C., Ajayi, O.O., Amund, O.O., 2016. Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas. Brazilian Journal of Microbiology 47:551-562.
Alexander, M., 1999. Biodegradation and bioremediation. Gulf Professional Publishing, San Diego. 453 p.
_||_
Guarino, C., Spada, V., and Sciarrillo, R., 2017. Assessment of three approaches of bioremediation (Natural Attenuation, Landfarming and Bioagumentation – Assistited Landfarming) for a petroleum hydrocarbons contaminated soil. Chemosphere 170:10-16.
Mirsal Ibrahim, A., 2008. Soil pollution: origin, monitoring and remediation, 1st Ed., Springer, Germany. 312 p.
Sajna, K.V., Sukumaran, R.K., Gottumukkala, L.D., and Pandey, A., 2015. Crude oil biodegradation aided by biosurfactants from Pseudozyma sp. NII 08165 or its culture broth. Bioresource Technology 191:133-139.
Zhu, XAD., Venosa, MT., and Suidan, M., 2004. Literature review on the use of commercial bioremediation agents for cleanup of oil-contaminated estuarine environments. EPA/600/R-04/075.
Yang, S Z., Jin, HJ., Wei, Z., He, RX., Ji, YJ., Li, XM., and Yu, SP., 2009. Bioremediation of oil spills in cold environments: A review. Pedosphere. 19(3): 371–381.
Minoui, S., Minai-Tehrani, D., Eslami, G., and Sobhani-Damavandifar, Z., 2009. Change in cytochromes content of Pseudomonas sp. in the medium containing petroleum. Australian Journal of Basic and Applied Sciences, 3(3):1512-1516.
Tekorienė, R., 2008. Distribution of the genus Pseudomonas bacteria in oil-polluted soil, water, polymeric materials, plant remnants and food products. Ekol. 54(3):143–148.
Taoufik, J., Zeroual, Y., Moutaouakkil, A., Moussaid, S., Dzairi, FZ., Talbi, M., Hammoumi, A., Belghmi, K., Lee, K., Loutfi, M., and Blaghen, M., 2004. Aromatic hydrocarbons removal by immobilized bacteria (Pseudomonas sp., Staphylococcus sp.) in fluidized bed bioreactor. Annals of Microbiology, 54:189-200.
Guieysse, B., Viklund, G., Toes, AC., and Mattiasson, B., 2004. Combined UV-biological degradation of PAHs. Chemosphere, 55(11):1493-1499.
Nnamchi, CI., Obeta, JAN., and Ezeogu, LI., 2006. Isolation and characterization of some poly aromatic hydrocarbon degrading bacteria from Nsukka soils in Nigeria. Int. J. Environ. Sci. Tech. 3(2): 181-190.
Bayat, Z., Hassanshahian, M., Hesni, M.A., 2015. Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf. Marine Pollution Bulletin 101, 85-91.
Di Martino, C., López, N.I., and Raiger Iustman, L.J., 2012. Isolation and characterization of benzene, toluene and xylene degrading Pseudomonas sp. selected as candidates for bioremediation. International Biodeterioration & Biodegradation 67:15-20.
Ma, K.-Y., Sun, M.-Y., Dong, W., He, C.-Q., Chen, F.-L., and Ma, Y.-L., 2016. Effects of nutrition optimization strategy on rhamnolipid production in a Pseudomonas aeruginosa strain DN1 for bioremediation of crude oil. Biocatalysis and Agricultural Biotechnology 6:144-151.
Shim, H., Hwang, B., Lee, SS., and Kong, SH., 2005. Kinetics of BTEX biodegradation by a coculture of Pseudomonas putida and Pseudomonas fluorescens under hypoxic conditions. Biodegradation, 16: 319–327.
Walkey, A., and Black, IA., 1934. An Examination of the Degtjareff Method for Determining Soil Organic Matter and a Proposed Modification of the Chromic Acid Titration Method. Soil Science, 37: 29-38.
Olsen, SR., and Sommers, LE., 1982. Phosphorus. In: Page AL. (ed), Methods of Soil Analysis, Agron. No. 9, Part 2: Chemical and microbiological properties, 2nd ed., Am. Soc. Agron., Madison, pp: 403- 430.
Page, AL., Miller, RH., and Keeney, DR., 1982. Method of soil analysis (part 2: chemical & microbiological properties). American society of Agronomy, Madison, 1121 p.
Atagana, HI., Haynes, RJ., and Wallis, FM., 2003. Optimization of soil physical and chemical conditions for the bioremediation of creosote-contaminated soil. Biodegradation, 14(4): 297-307.
USEPA. 2001. Guideline for the bioremediation of marine shorelines and fresh water wetland. Office of research and development, US Environmental Protection Agency.
Eaton, AD., and Franson, MAH., 2005. Standard Methods for the Examination of Water & Wastewater: American Public Health Association. Washington DC. 1020 p.
Asrari, E., Tavallali, H., and Hagshenas, M., 2010. Removal of Zn (II) and Pb(II) ions Using Rice Husk in Food Industrial Wastewater, Journal of Applied Sciences and Environmental Management, 14(4): 159–162.
Safahie, A., Mojudi, F., and Zolgharnain, H., 2011. Evaluation and Comparision of the Ability of Pseudomonas strain indigenous to Khormousa in removal of Aromatic Cmpounds. Journal of Environmental Studies, 58:149-158. (In Persian).
Naji Rad, S., Alikhani, HA., Savaghebi, GR., Saleh-Rastin, N., and Farahani, M., 2006. Bioremediaton of Soil Contaminated to Petroleum Hydrocarbons. Master of Science Thesis, University of Tehran. (In Persian)
Das, k., and Mukherjee, K., 2007. Crude petroleum-oil biodegradation eyciency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India. Bioresource Technology 98: 1339-1345.
Kucerova, R., 2006. Application of pseudomonas putida and rhodococcus sp. By biodegradation of PAH(s), PCB(s) and nel soil samples from the hazardous waste dump in pozďátky (czech republic). Rud. Geol. Naft. 18:97-101.
Liu, B., Ju, M., Liu, J., Wu, W., Li, X., 2016. Isolation, identification, and crude oil degradation characteristics of a high-temperature, hydrocarbon-degrading strain. Marine Pollution Bulletin 106:301-307.
Bushnaf, K.M., Puricelli, S., Saponaro, S., Werner, D., 2011. Effect of biochar on the fate of volatile petroleum hydrocarbons in an aerobic sandy soil. Journal of Contaminant Hydrology 126:208-215.
Varjani, S.J., 2017. Microbial degradation of petroleum hydrocarbons. Bioresource Technology 223:277-286.
Yuan, SY., Wei, SH., and Chang, BV., 2000. Biodegradation of polycyclic aromatic hydrocarbons by a mixed culture. Chemosphere 41:1463–1468.
Nwinyi, O.C., Ajayi, O.O., Amund, O.O., 2016. Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas. Brazilian Journal of Microbiology 47:551-562.
Alexander, M., 1999. Biodegradation and bioremediation. Gulf Professional Publishing, San Diego. 453 p.
