Bacterial Bioremediation of Surficial Oil- Contaminated Soil in Tabriz Refinery Campus
Subject Areas : soil pollutionRezvan Agha mohammadi 1 , Kamal Siahcheshm 2 , Gholamreza Zarrini 3 , Ali Kadkhodaee 4
1 - M.Sc., Environmental Geology, Earth Sciences Dept., Natural Sciences Faculty, University of Tabriz.
2 - Associate Professor, Economic Geology, Earth Sciences Dept., Natural Sciences Faculty, University of Tabriz. *(Corresponding Author)
3 - Associate Professor, Microbiology, Natural Sciences Faculty, University of Tabriz.
4 - Associate Professor, Petroleum Geology, Natural Sciences Faculty, University of Tabriz.
Keywords: Oil pollution, soil, Bacterial Bioremediation, Tabriz refinery,
Abstract :
Background and Objective: Nowadays, the use of bacteria to clean oil pollution from water and soil has been considered by scientists and this method is more economical than other methods and does not cause toxic compounds in the environment. Method: In the present study, the results of the bioremediation process have been presented to identify the most optimal bacterial isolates of petroleum degraders in physicochemical and textural conditions of contaminated soil of Tabriz refinery. Gas chromatographic analysis with thermal detection (Gc-FID) was used to evaluate the removal rate of hydrocarbons. Findings: The 7 isolates of bacteria were purified in this study. A Pseudomonas isolate was introduced as a strain with the highest efficiency. Discussion and Conclusion: After 30 days, as a result of Pseudomonas and Acinetobacter performance, the amount of TPH in contaminated soil samples was decreased in rate of 65.25 and 42.39 percent, respectively. Based on this investigation, the Bacterial Bioremediation method to clean up contaminated soils by petroleum hydrocarbons due to simplicity of implementation, environmentally friendly, safety and low cost is highly proposed to conduct in other oil refineries in Iran.
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- Shah Alian, F., Safahiyeh, A., Salamat, N., Mojudi, F., Zare Dust, M., 2015. Investigating the role of isolated bacteria from Gulf precipitates in biological removal of oil pollutants (Antracene), Journal of Environmental Science and Engineering 1(4), pp. 11-17 (In Persian).
- Johnsen, A., R and Karlson, U., 2004. Evaluation of bacterial strategies to promote the bioavailability of polycyclic aromatic hydrocarbons. Applied microbiology and biotechnology, 63, pp. 452-459.
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- Andreoni, V., Gianfreda, L., 2007. Bioremediation and monitoring of aromatic-polluted habitats. Applied Microbiology and Biotechnology, 76, pp. 287-308.
- Namkoong, W., Hwang E., Y., Park, J. S., Choi, J. Y., 2002. Bioremediation of diesel-contaminated soil with composting. Environmental Pollution, 119, pp.23-31.
- Alexander M, 1995. How toxic are toxic chemicals in soil. Environmental Science and Technology, 29: 2713–2717.
- Chan, H., 2011. Biodegradation of petroleum oil achieved by bacteria and nematodes in contaminated water. Separation and purification technology, 80, pp.459-466.
- Semprini, L., 1998. Current and potential applications of in situ bioremediation. Biotechnology for Soil Remediation, pp. 21-27.
- Priya, N. S., Doble, M., Sangwai, J. S., 2015. Bioremediation of Costal and Marine Pollution due to Crude Oil using a Microorganism Bacillus Subtilis. Procedia Engineering, 116, pp. 213-220.
- Abed, R. M., Al-Sabah,i J., Al-Maqrashi F,Al-Habsi, A., Al-Hinai, M., 2014. Characterization of hydrocarbon-degrading bacteria isolated from oil-contaminated sediments in the Sultanate of Oman and evaluation of bioaugmentation and biostimulation approaches in microcosm experiments: International Biodeterioration & Biodegradation 89, pp. 58-66.
- Lee, K., Merlin, X., 1999. Bioremediation of oil on shoreline environments: development of techniques and guidelines. Pure and applied chemistry, 71, pp. 161-171.
- Timmis, K., N and Pieper, D. H., 1999. Bacteria designed for bioremediation. Trends in biotechnology, 17, pp. 201-204.
- Coelho, F. J. R. C., Sousa, S., Santos, L., Santos, A. L., Almeida, A., GOMES, N. C., CUNHA, Â., 2010. PAH degrading bacteria in an estuarine system. Interdisciplinary studies on environmental chemistry—biological responses to contaminants, pp77-87.
- Shah Alian, F., Safahiyeh, A., Salamat, N., Mojudi, F., Zare Dust, M., 2015. Investigating the role of isolated bacteria from Gulf precipitates in biological removal of oil pollutants (Antracene), Journal of Environmental Science and Engineering 1(4), pp. 11-17 (In Persian).
- Johnsen, A., R and Karlson, U., 2004. Evaluation of bacterial strategies to promote the bioavailability of polycyclic aromatic hydrocarbons. Applied microbiology and biotechnology, 63, pp. 452-459.