Isolation and identification of dominant Salmonella species and their virulence factors in pets and their owners in Isfahan city

Subject Areas : Journal of Comparative Pathobiology

آتنابلالی Balali Dehkordi ¹ , علی Sharifzadeh ²

1 - Department of Microbiology, Faculty of Veterinary Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, I.R. Iran.
2 - Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, I.R. Iran

Received: 2022-10-21 Accepted : 2023-02-19 Published : 2023-02-19

Keywords: Salmonella, Dog, Cat, Virulence, Antibiotic resistance,

Abstract :

Salmonellosis is a common disease between humans and animals, and in some cases, animals carrying bacteria are a potential source of contamination for humans. The aim of this study was to assess the potential role of dogs and cats and their owners in epidemiology of salmonellosis in in Isfahan city. For this purpose, Rectal swabs of 115 asymptomatic dogs and cats from Isfahan city (2021) were cultured and evaluated by PCR. Salmonella isolates were serotyped and virulence gene and tested for antimicrobial susceptibility applying standard methods. The results obtained in the present study indicated that Salmonella spp. were isolated from 8% of cats,16% of cat owners and 12% of dog owners samples. 2 salmonella serotypes were observed including S. typhymurium (80%), and S. enteritidis (20%). The results showed that there is no significant relationship between age, condition of the digestive system and the environment of storage with the amount of salmonella infection in dogs and cats. Also, no connection was observed between the contamination of animals and their owners. According to the results, all cases were infected with invA and flic strains. According to the antibiogram tests performed, they were sensitive to ciprofloxacin, azithromycin, nalidixic acid, semi-sensitive to tetracycline, gentamicin, cephalothin   and resistant to ampicillin, amoxicillin, cephalexin. Isolation of Salmonella spp. from asymptomatic cats and dogs makes them dangerous source of Salmonella and a treat for human and animal health.

References:

1. Groisman EA, Ochman H. How Salmonella became a pathogen. Trends in microbiology. 1997;5(9):343-9.

2. Coburn B, Grassl GA, Finlay B. Salmonella, the host and disease: a brief review. Immunology and cell biology. 2007;85(2):112-8.

3. Besser JM. Salmonella epidemiology: A whirlwind of change. Food microbiology. 2018;71:55-9.

4. Reimschuessel R, Grabenstein M, Guag J, Nemser SM, Song K, Qiu J, et al. Multilaboratory survey to evaluate Salmonella prevalence in diarrheic and nondiarrheic dogs and cats in the United States between 2012 and 2014. Journal of clinical microbiology. 2017;55(5):1350-68.

5. Weiss G, Schaible UE. Macrophage defense mechanisms against intracellular bacteria. Immunological reviews. 2015;264(1):182-203.

6. Jajere SM. A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors, host specificity and antimicrobial resistance including multidrug resistance. Veterinary world. 2019;12(4):504.

7. Johnson R, Mylona E, Frankel G. Typhoidal Salmonella: Distinctive virulence factors and pathogenesis. Cellular Microbiology. 2018;20(9):e12939.

8. Lim Y-H, Hirose K, Izumiya H, Arakawa E, Takahashi H, Terajima J, et al. Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar Typhimurium. Japanese journal of infectious diseases. 2003;56(4):151-5.

9. Namroodi S, Staji H, Sharafi S. Survey on Salmonella contamination of Golden jackals by microbiological culture methods and PCR in Golestan and Mazandaran Provinces. Journal of Veterinary Research. 2017;72(3).

10. Kocabiyik A, Cetin C, Dedicova D. Detection of Salmonella spp. in stray dogs in Bursa Province, Turkey: first isolation of Salmonella Corvallis from dogs. Journal of Veterinary Medicine, Series B. 2006;53(4):194-6.

11. Namroodi S, Estaji H, Dehmordeh M. Frequency and antimicrobial resistance pattern of Salmonella Spp in asymptomatic rural dogs. Journal of Mazandaran University of Medical Sciences. 2016;26(135):153-7.

12. EMADI CS, Hasanzadeh M, BOZORG MM, Mirzaei S. Characterization of the Salmonella isolates from backyard chickens in north of Iran, by serotyping, multiplex PCR and antibiotic resistance analysis. 2009.

13. Salehi TZ, Badouei MA, Madadgar O, Ghiasi SR, Tamai IA. Shepherd dogs as a common source for Salmonella enterica serovar Reading in Garmsar, Iran. Turkish Journal of Veterinary & Animal Sciences. 2013;37(1):102-5.

14. Bayatmakoo Z, Bayatmakoo R. A study multi-drug resistant of salmonella Typhi strains Tabriz. Journal of Ardabil University of Medical Sciences. 2004;4(1):12-7.

15. Organization WH. Antimicrobial resistance: global report on surveillance: World Health Organization; 2014.

16. Vaez H, Ghanbari F, Sahebkar A, Khademi F. Antibiotic resistance profiles of Salmonella serotypes isolated from animals in Iran: a meta-analysis. Iranian Journal of Veterinary Research. 2020;21(3):188.

17. Kumarss A, Taghi ZS, Gholamreza N, Reza R, Javid A, Shahrnaz BA. Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from human and animals in Iran. African Journal of Microbiology Research. 2010;4(21):2202-10.

18. Nashwa MH, Mahmoud A, Sami SA. Application of multiplex polymerase chain reaction (MPCR) for identification and characterization of Salmonella Enteritidis and Salmonella Typhimurium. J Appl Sci Res. 2009;5:2343-8.

19. Van Kessel J, Karns J, Gorski L, McCluskey B, Perdue M. Prevalence of Salmonellae, Listeria monocytogenes, and fecal coliforms in bulk tank milk on US dairies. Journal of Dairy Science. 2004;87(9):2822-30.

20. Rastegar H, Gharemani M, Hallaj S, Jalali M, Anjarani S, Khosrokhavar R. Isolation and identification of Salmonella typhimurium in milk by conventional and PCR methods. Iranian Journal of Nutrition Sciences and Food Technology. 2008;3(3):45-52.