Study of kidney anatomy and microscopic of renal proximal and distal tubules of middle and posterior part of kidney tissue in Oncorhynchus mykiss and Astronotus ocellatus

Subject Areas : Journal of Comparative Pathobiology

. S Mohammadalipour ¹ , Iraj pousty ² , , S Hesaraki ³

1 - Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Faculty of science and research university
3 - Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2021-11-01 Accepted : 2021-11-01 Published : 2020-11-21

Keywords: histology, Anatomy, Kidney, Oncorhynchus mykiss, Astronotus Ocellatus,

Abstract :

The present study was conducted to compare the histology of renal proximal tubules (I) and (II) in two species (Rainbow trout and Oscar). Rainbow trout, known as Oncorhynchus mykiss scientific name from the Salmonidae family which is cultivated and freshwater. Oscar fish, the scientific name is Astronotus Ocellatus, which is one of the most well-known ornamental fish of the Chichlidae family. Five piece of the two fish species were selected randomly and their macroscopic specification and morphometric measurements were recorded. Samples were fixed in %10 formalin buffer and the cuts were prepared with a thickness of five micron and then they were stained by Hematoxylin and eosin and Periodic acid–Schiff method and studied by light microscopy. The data were analyzed with SPSS software and T-Test statistical test (P <0.05). Proximal and distal tubes were detected and the variables were measured. The size of the cell nucleus and the outer diameter of the proximal (I) had a significant difference (P<0.01). There has been significant difference in the number and the size of cell and its nucleus, the inner and outer diameter of the proximal II between two species (P<0.001). The number, cell size of the distal tubules has been significant difference between two species (P<0.01). The size of the cell nucleus, the inner and outer diameter of the distal tubules have been significant difference between two species (P<0.001). According to the results, the anatomy and histology of kidney tissue are different in the Rainbow trout and Oscar fish. 

References:

1. Schlenk D. , Benson W.H. New Perspectives: Toxicology and the Environment: Target Organ Toxicity in Marine and Freshwater Teleosts. CRC Press, Taylor & Francis Us. 2001; 93-98


2. Baltz D. M. Introduced fishes in marine systems and inland seas. Biological Conservation, 1991; 56: 151-177.


3. Pronek N. translated by Emami F & Heidaripour S. Oscars. Scientific-Research Journal of Experimental Animal Biology. 2015; 4:12-41, 56-61


4. Morovvati H , Khaksary Mahabady M , Mesbah M, Fakoori R. Anatomical and histological study of kidney in Barbus grypus. Journal of Marine Science and Technology, 2017; 16(2): 49-61


5. Takashima F. An atlas of fish histology: Normal and pathological features. 2nd ed, 8th chapter. 1994; 171-174, 294-303.


6. Roberts, R. J. Fish pathology. 4th ed. Wiley-Blackwell publishing Ltd. Chapter2: the anatomy and physiology of teleosts. 2012; 35-90


7. Roomiani E, Abdi R, Zolgharnein H, Savari A, Morovvati H. Localization and Effect of Hyper and Hypo-osmotic Environments on Distribution of Na+, K+- ATPase in Kidney Tubules of Juveniles of (Epinephelus coioides) Na+/K+-ATPase Epinephelus Coioides . Journal of Cell & Tissue (JCT). 2012; 2(4): 407-414


8. Eslami M., Movahedinia A., Rounagh M. Study of anatomical variety and tissue structure of kidney in som  fish  spicies  from  the  north  of  the Persian  Gulf. Aquatic Ecology Magazine. 2016; 8(1); 104-112


9. Anthony L. Mescher, McGraw Hill NY, Text and Atlas: Junquiera’s Basic Histology,13 th ed.2013;1-5,385-401


10. Krayushkina L. S. Characteristics of osmotic and ionic regulation in marine diadromous sturgeons Acipenser brevirostrum and Acipenser oxyrhynchus (Acipenseridae). Ichthyology, 1998; 38: 660 -668.


11. Cataldi E., Garibaldi L., Crosetti D., Leoni C.& Cataudella S. Variations in renal morphology during adaptation to salinities in tilapia. Environmental Biology of Fishes, 1999; 31: 101-106.


12. Charmi A., Parto P., Bahmani M. and Kazemi R. Morphological and Histological Study of Kidney in Juvenile Great Sturgeon (Huso huso) and Persian Sturgeon (Asipenser persicus). Agricltural and Enviromental Sciences, 2010; 7: 505-511


13. Morovvati H., Erfanimajd N., Peyghan R., Mobaraki GH. Histological Study of Exceretory portion of kidney in Grass Carp (Ctenopharygodon Idella). Scientific Research Iranian Veterinary Journal. 2011; 4 (29):25-32


14. Tang C. H., Wu W. Y., Tsai S. C., Yoshinaga T. and Lee, T. H. Elevated Na+/K+-ATPase responses and its potential role in triggering ion reabsorption in kidneys for homeostasis of marine euryhaline milkfish (Chanos chanos) when acclimated to hypotonic fresh water. Comparative Physiology and Biochemistry, 2010; 180: 813 -824.


15. Khalil R. M. Histological studies on the kidneys of the red sea Syngnathiformes fishes. Society of Zoology Egyptian Bulletin, 1979; 29: 28-36.


16. Tojar S., khodadadi M., Javaheri M. Compare the appropriate doses of three anesthetics: 2-phenoxyethanol, clove extract and PI22 in silver carp. Journal of Marine Science and Technology Research.  2011; 6(3): 41-52


17. Kessel R.G. Basic medical histology: The Biology of Cells tissue and organs. New York: Oxford University Press. Chapter 1 and 2. 1998; 1-4, 452-469


18. Bancroft J.D. & Gamble M. Theory and practice of histological techniques. Fifth edition, 2002; 80,85-97,113,153.

 

19. Luna HT. Manual of Histologic Staining of the armed forces institute of pathology. New York: Blackiston Division: mc grow hill. 3th ed. 1968;1-46


20. Ostrandef G. K. The laboratory, 1st ed. Diego Academic press. 2000.


21. Lőw P.; Molnár K. & Kriska G. Atlas of Animal Anatomy and Histology. Springer Cham Heidelberg New York Dordrecht, London Springer International Publishing Switzerland, 2016; ix


22. Aughey E. & L Frye,F. Comparative veterinary histology with clinical correlates. Manson publishing, CRC Press , 2001;137-142


23. Mumford S., Heidel J., Smith C., Morrison J., MacConnell B & Blazer V. Fish histology and histopathology. Chapter2, 2007p:1-10


24. Krayushkina L. S., Panov A. A., Gerasomov A. A. & Potts W. T. W. Changes in sodium, calcium and magnesium ion concentrations in sturgeon (Huso huso) urine and in kidney morphology. Comparative Physiology and Biochemistry, 1996; 165: 527-533.


25. Baustian M. D. and Beyenbach K. W. Natriuretic peptides and the acclimation of aglomerular toadfish to hypo-osmotic media. Compparative Physiology and Biochemistry, 1979;169: 507-514.


26. Miraali A, Movahedinia E, Abdi R, Salati EP. Investigation of the tissue structure of all Sparidentex hasta. Scientific-Research Journal of Marine Biology, Ahvaz Branch. 2013; 5(18); 71-80


27. Khodabandeh S., Khoshnood Z. and Mosafer S. Immunolocalization of Na+/K+- ATPase-rich cells in the gill and urinary system of Persian sturgeon (Acipenser persicus), fry. Aquaculture Research, 2009; 40: 329 -336.


28. Chenari F, Morovvati H. , Ghazilou A. , Savari A. , Ronagh M. Rapid variation in kidney histology in spotted scat Scatophagus argus on exposed to abrupt salinity changes. Iranian Journal of Veterinary Research, 2011; 12( 3):256-259


29. Heidari B, Avarjeh S, Taghavi Jelodar H. The combined effects of gradual changes in salinity and temperature on gill tissue of common carp (Cyprinuscarpio). Journal of Aquatic Physiology and Biotechnology. 2015; 2:80-94


30. Azizi S, Kochanian P, Peyghan R, Khansari A, Darvish Bastami K. Effects of different salinities on histological changes of kidney in (Cyprinus carpio L.). Journal of Animal Environment, 2010;1(3):47-50