Comparison of Serumic Concentration of Vitamin A and Beta-Carotene in the Serum Positive Against Peste des Petits Ruminant and Healthy Dams

Subject Areas : Journal of Comparative Pathobiology

, A.  Ganjkhanloo ¹ (Resident of Large Animal Internal Medicine, Department of Clinical Pathology and Large Animal Internal Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.)
, A.  Hassanpour ² (Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran)
, Sh  Agajani ³ (دانشجوی دوره دکتری عمومی دامپزشکی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران)

Received: 2020-11-07 Accepted : 2020-11-07 Published : 2020-05-21

Keywords: Serum, Sheep, Vitamin A, Beta-carotene,

Abstract :

Peste des petits ruminants (PPR) is an economically important viral disease between sheep and goats that is endemic in Iran. This study was performed to Comparison of Serumic Concentration of Vitamin A and Beta-Carotene in the Serum Positive Against Peste des Petits Ruminant and Healthy Dams in sheep and goats in healthy animals in Khodabandeh area in summer of 2018.During this study, 200 samples were collected from Jugular veins of sheep (150) and goats (50) which were not vaccinated against PPR disease and after serum isolation the samples were analyzed using competitive ELISA kit (ID.vet French). Seroprevalence of PPR in sheepwas 108 cases (72%) and in goats was 36 cases (72%). Seropositive animals were placed in the sick group (144 animals) and Seronegative animals were placed in the healthy group (56 animals). Then Serum levels of Vitamin A and Beta-Carotene measured in all serum samples by hexane method. The mean and standard deviation of vitamin A and Beta-Carotene in serum was 27/6 ± 4/1 mg/dl and 19/5 ± 3/2 mg/dl in diseased group and 58/9 ± 3/2 mg/dl and 19/8 ± 2/1 mg/dl in healthy group, Respectively. Serum level of vitamin A was significantly different between healthy and diseased animals (p<0.05), but Beta-Carotene serum level was not significantly different. The final result is that given the low levels of vitamin A in animals infected with the disease, in areas where the disease is contagious, it can be administered injectable form to provide this vitamin and strengthen the immune system.

References:

1. Constable PD, Hinchliff KW, Done SH and Grnberg W. Vitamin A Deficiency. Veterinary Medicine. 11th ed. London: Bailliere Tindall; 2017. p. 1314-20.


2. Munir M, Abubakar M, Khan M, Abro S. Comparative efficacy of Single Radial Haemolysis Test And Countercurrent Immunoelectroosmo-Phoresis with Monoclonal Antibodies-Based Competitive Elisa for the Serology of Peste Des Petits Ruminants In Sheep And Goats. Bulgarian journal of veterinary medicine. 2009;12(4): 246-253.


3. Foroughi A, Chaharaein B, Ownagh A, Mardani K. Comparison of c-ELISA and RT-PCR Methods in Detection of Peste Des Petits Ruminants in Early Stage from Clinical Specimens in Goats and Sheep in Kermanshah Province. Quarterly Journal of Experimental Animal Biology. 2013;1(4):51-56. [In Persian]


4. Couacy-Hymann E, Roger F Fau - Hurard C, Hurard C Fau - Guillou JP, Guillou Jp Fau - Libeau G, Libeau G Fau - Diallo A, Diallo A. Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. 2016;(0166-0934 (Print)).


5. Suzuki J, Katoh N. A simple and cheap method for measuring serum vitamin A in cattle using only a spectrophotometer. Japanese Journal of Veterinary Science. 1990;52(6):1281-3.


6. Rasooli A, Nouri M, Esmaeilzadeh S, Ghadiri A, Gharibi D, Javaheri Koupaei M, et al. Occurrence of purulent mandibular and maxillary osteomyelitis associated with Pseudomonas aeruginosa in a sheep flock in south-west of Iran. Iranian Journal of Veterinary Research. 2018;19(2):133-6.

 

7. Kihu SM, Gachohi JM, Ndungu EK, Gitao GC, Bebora LC, John NM, et al. Sero-epidemiology of Peste des petits ruminants virus infection in Turkana County, Kenya. BMC Vet Res. 2015;8(11):87- 94..


8. Yousuf MA, Rahman MM, Alauddin M, Rahman SB, Islam SS, Islam MR, et al. Sero-surveillance of peste des petits ruminant viral antibody in goats at different areas of Bangladesh. Asian Journal of Medical and Biological Research. 2017;3(3):347-51.


9. Kgotlele T, Torsson E, Kasanga C, Wensman JJ, Misinzo G. Seroprevalence of Peste Des Petits Ruminants virus from samples collected in different regions of Tanzania in 2013 and 2015. 2016; 7(6):1-5.


10. Farougou S, Gagara M, Mensah GA. Prevalence of peste des petits ruminants in the arid zone in the Republic of Niger. Onderstepoort Journal of Veterinary Research. 2013; 80(1): 324-331.


11. Das KK, Shil NK, Islam MR. Sero-epidemiological investigation on Peste des Petits Ruminants in black Bengal goats. Bangladesh journal of microbiology. 2007;24(2):143-5.


12. Balamurugan V, Krishnamoorthy P, Raju DSN, Rajak KK, Bhanuprakash V, Pandey AB, et al. Prevalence of Peste-des-petits-ruminant virus antibodies in cattle, buffaloes, sheep and goats in India. Virusdisease. 2014;25(1):85-90.


13. Ali AE-RA. Residual effect of heavy metals due to used drinking water polluted with sewage on health performance and blood serum antioxidant vitamins in sheep and goats in Assiut governorate. Ass Univ Bull Environ Res. 2005;8(1):41-50.


14. Mert H, Kozat S, Mert N, Değer Y. Vitamin status in yearling rams with growth failure. Turkish Journal of Veterinary and Animal Sciences. 2008;31(6):407-9.


15. Hedayat N, Ghadrdan MA, Shahriari A, Zarei M. Determination of beta-carotene and vitamin A contents of serum and liver of sheep slaughtered in Ahvaz abattoir during different seasons of the year. Veterinary Clinical Pathology. 2016;10(39):213-24.


16. Afshari G, Hassanpour A, Haghpanah H, Amoughli-Tabrizi B. Seasonal variation of vitamin A and Beta-carotene levels in Ghezel sheep. Turkish Journal of Veterinary and Animal Sciences. 2008;32(2):127-9.


17. Mashayekhi M, Khayat NM, Ebadi A, Panahi F. Comparison of the serumic levels of vitamin A, vitamin C and zinc between apparently healthy and those affected by febrile pneumonia in Ghezel sheep. Journal of Veterinary Clinical Pathology. 2010;4(3):883-90.


18. Kataria A, Kataria N. Evaluation of oxidative stress in sheep affected with peste des petits ruminants. Journal of Stress Physiology & Biochemistry. 2012;8(4):143-9.


19. Herdt TH, Stowe HD. Fat-soluble vitamin nutrition for dairy cattle. Veterinary Clinics of North America: Food Animal Practice. 1991;7(2):391-415.


20. Remillard R, Stem E, Michel K, Engelking L, Sollod A. Oral vitamin A supplementation to debilitated cattle during Sahelian dry seasons. Prev Vet Med. 1990;9:173-83.