Study on effects of selenium on histopathology and histometrical parameters in testis of normal and experimental varicocele rats

Subject Areas : Journal of Comparative Pathobiology

Taghizadeh, L., Eidi, A., Mortazavi, P., Haeri Rohani, A.  . ¹ (.)

Received: 2018-12-20 Accepted : 2018-12-20 Published : 2018-11-22

Keywords: Sodium selenite, Varicocele, Testis, Rat,

Abstract :

Negative effects of oxidative stress on serum, semen and testicular tissue have shown in patients with varicocele or in animal models. This study investigated the effects of sodium selenite on histological and histometrical parameters in testis of normal and varicocele-induced male Wistar rats. Forty-four rats were divided randomly in 11 groups: control group (1 ml/kg of saline intraperitoneally), sham-operated control group (only surgery without varicocele, 1 ml/kg of saline intraperitoneally, experimental varicocele groups (sodium selenite at doses 0.05, 0.1, 0.2, and 0.4 mg/kg, intraperitoneally) and normal experimental groups (sodium selenite at doses 0.05, 0.1, 0.2, and 0.4 mg/kg, intraperitoneally). Two months after surgery using Turner method, varicocele was induced in rats. Then, sodium selenite was administered continuously for 60 days. Testis tissue samples were collected for histological and histometrical studies. Sodium selenite caused a significant increase in thickness of epithelium of seminiferous tubules compared to varicocele control group (P<0.01). Tubular differentiation index (TDI) and spermiogenesis index (SI) of seminiferous tubules showed an increase in sodium selenite groups compared to normal control group. TDI and SI of seminiferous tubules showed a significant decrease in varicocele control group compared to normal control group (P<0.05). Sodium selenite caused a significant increase in number of leydig cells in varicocelized rats, compared to varicocele control group (P<0.01). Taken together, the results of this study suggest that selenium treatment may have beneficial effect on the testis tissue of varicocelized rats.

References:

1- مروتی، ح.، مرادی، ح.ر.، ادیب مرادی، م.، شیبانی، م.ت.، سالار آملی، ج. (1396): تأثیر عصاره جوانه گندم بر ساختار هیستولوژیک و هیستومتریک بیضه و پارامترهای اسپرم موش صحرایی مواجهه شده با سرب، مجله دانشکده دامپزشکی تهران، 72 (1): 101-87.

2- Agarwal, A., Sharma, R.K., Desai, N.R., Prabakaran, S., Tavares, A., Sabanegh, E. (2009): Role of oxidative stress in pathogenesis of varicocele and infertility. Urology. 73(3): 461-469.

3- Azizollahi, G., Azizollahi, S., Babaei, H., Kianinejad, M., Baneshi, M.R., Nematollahi-mahani, S.N. (2013): Effects of supplement therapy on sperm parameters, protamine content and acrosomal integrity of varicocelectomized subjects. J. Assist. Reprod. Genet. 30(4): 593-599.

4- Behne, D., Weiler, H., Kyriakopoulos, A. (1996): Effects of selenium deficiency on testicular morphology and function in rats. J. Reprod. Fertil. 106(2): 291-297.

5- Cameron, D.F., Snydle, F.E., Ross, M.H., Drylie, D.M. (1980): Ultrastructural alterations in the adluminal testicular compartment in men with varicocele. Fertil. Steril. 33(5): 526-533.

6- Chabory, E., Damon, C., Lenoir, A., Henry-Berger, J., Vernet, P., Cadet, R., Saez, F., Drevet, J.R. (2010): Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity1. J. Anim. Sci. 88(4): 1321-1331.

7- De Stefani, S., Silingardi, V., Micali, S., Mofferdin, A., Sighinolfi, M.C., Celia, A., Bianchi, G., Giulini, S., Volpe, A., Giusti, F. (2005): Experimental varicocele in the rat: early evaluation of the nitric oxide levels and histological alterations in the testicular tissue. Andrologia. 37(4): 115-118.

8- Dorostghoal, M., Seyyednejad, S., Jabari, A. (2014): Protective effects of Fumaria parviflora L. on lead‐induced testicular toxicity in male rats. Andrologia. 46(4): 437-446.

9- Garg, H., Kumar, R. (2016): An update on the role of medical treatment including antioxidant therapy in varicocele. AJA. 18(2): 222-228.

10- Hamada, A., Esteves, S.C., Agarwal, A. (2015): Varicocele and male infertility: current concepts, controversies and consensus. Springer, USA.

11- Hsu, G.L., Ling, P.Y., Hsieh, C.H., Wang, C.J., Chen, C.W., Wen, H.S., Huang, H.M., Einhorn, E.F. (2005): Tseng, Guo-Fang. Outpatient varicocelectomy performed under local anesthesia. AJA. 7(4): 439-444.

12- Kass, E.J., Chandra, R.S., Belman, A.B. (1987): Testicular histology in the adolescent with a varicocele. Pediatrics. 79(6): 996-998.

13- Morovvati, H., Najafzadehvarzi, H., Rashidi, K. (2013): Effect of urtica dioica extract on histological and histometrical changes of testis of hamster after testosterone administration. ZJRMS. 15(11): 4-8.

14- Mostafa, T., Anis, T., El‐Nashar, A., Imam, H., Othman, I.A. (2001): Varicocelectomy reduces reactive oxygen species levels and increases antioxidant activity of seminal plasma from infertile men with varicocele. Int. J. Androl. 24(5): 261-265.

15- Olson, G.E., Winfrey, V.P., Hill, K.E., Burk, R.F. (2004). Sequential development of flagellar defects in spermatids and epididymal spermatozoa of selenium-deficient rats. Reproduction. 127(3): 335-342.

 16- Rezvanfar, M., Sadrkhanlou, R., Ahmadi, A., Shojaei-Sadee, H., Rezvanfar, M., Mohammadirad, A., Salehnia, A., Abdollahi, M. (2008): Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress. Hum. Exp. Toxicol. 27(12): 901-910.

17- Sun, H.J., Rathinasabapathi, B., Wu, B., Luo, J., Pu, L.P., Ma, L.Q. (2014): Arsenic and selenium toxicity and their interactive effects in humans. Environ. Int. 69: 148-158.

18- Treuting, P.M., Dintzis, S.M. (2011): Comparative anatomy and histology: a mouse and human atlas (expert consult). Academic Press, USA. P: 285-308.