Biochemical structure and antibacterial activity of hemolymph in male and female of fresh water prawn (Macrobrachium nipponense (

Subject Areas : Journal of Comparative Pathobiology

Karimzadeh, K.*, Zahmatkesh, A., Pormehr, M. . ¹ ( . )

Received: 2018-04-13 Accepted : 2018-04-13 Published : 2018-03-21

Keywords: antibacterial activity, hemolymph, electrophoresis, disk diffusion,

Abstract :

The aim of this study was to characterize the biochemical structure and bactericidal activity of hemolymph Microbrachium in different doses on several human bacterial pathogenFirst 80 individual (40 for male and 40 for female) of prawn (M. nipponense) with an average length of 8.1 ± 0.12 were collected from Anzali wetland. After separation of males and female from each other, hemolymph of samples were taken from ventral sinus of the prawn and structure of collected hemolymph was determined through an electrophoresis and FTIR analysis.The antibacterial activity of M. nipponense hemolymph was investigated against five pathogenic bacteria (Vibrio cholera, Klebsiella pneumonia, Bacillus subtilis, Staphylococcus aureus, Escherichia coli) in doses of 25 to 100 micrograms per liter by disk diffusion method.The electrophoretic pattern of males and females hemolymph revealed presence of proteins with molecular weights between 22-100 Kda. Hemolymph structure contained both secondary regular and irregular protein structures by FTIR analysis. Different doses of hemolymph, indicated inhibitory activity against different bacterial strains. Also significant difference was observed between male and female hemolymph in case of antibacterial activity p <0.05).Highest inhibitory effect of hemolymph was shown against Staphylococcus aureus, Bacillus subtilis and Vibrio cholera in male. While the lowest level of antibacterial activity was recorded in female against Bacillus subtilis and Escherichia coli with zone of inhibition 5.9 ± 0.12 and 6.5 ± 0.08 mmResults of this study indicated that fresh water prawn hemolymph was shown well bactericidal activity and can be a substitute for antibiotic-based chemical drugs.

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