Effect of dietary inclusion of Mentha piperita on histomorphometric parameters of bursa of Fabricius, cecal tonsils and thymus of Broiler chickens

Subject Areas : Journal of Comparative Pathobiology

Hamedi, S., Shomali, T., Zeinali Tajani, R.  . ¹ (.)

Received: 2017-06-18 Accepted : 2017-06-18 Published : 2017-09-23

Keywords: histology, Menthea piperita, Broiler, Lymphatic organs,

Abstract :

To evaluate immunostimulatory effects of Mentha piperita on histological characteristics of bursa of Fabricius, cecal tonsils and thymus of broilers 40 male one-day old Ross 308 chickens were randomly allocated into 5 groups of 8 birds in separate cages. Four groups received dried M. piperita powder at 10, 20, 40 and 80 g/kg of diet while group 5 birds were kept as control and fed with standard diet. After 6 weeks all birds were slaughtered and samples of bursa, cecal tonsils and thymus were removed. Six-micrometer thick slides were made and stained with H&E for evaluation of histomorphometrical parameters by a linear graticule under light microscope. Data analysis was performed by ANOVA method. Consumption of diet supplemented with M. piperita resulted in a dose-dependent significantly increase in number of follicles and height of plicae in bursa. Villi became shorter and wider in cecal tonsils while the density of follicular units significantly increased. Lobular thickness increased in thymus. The results of this study demonstrate that dietary consumption of M. piperita during rearing period is associated with expansion and stimulation of histological immune structures of bursa, cecal tonsils and thymus in broiler chickens.

References:

1. امین، غ. (۱۳۷۰): گیاهان دارویی ایران، چاپ اول، انتشارات معاونت پژوهشی وزارت بهداشت و آموزش پزشکی، صفحه: 230.


2. دوستی، ا.، طاهرپور، ک.، نصر، ج.، قاسمی،  ح. (1392): بررسی اثر جیره های حاوی نعناعع، پروبیوتیک یا پریبیوتیک بر عملکرد و برخی فراسنجه های بیوشیمیایی خون جوجه های گوشتی، نشریه علوم دامی، 101: 91-100

1. AL-kassie, G.A.M (2008): The effect of anis and rosemary on broiler performance. Inter. J. poult. Sci. 7(3):243-245.


2. Amasaib, E.O., abd elrahman, B.H., abdelhameed, A.A., atta elmnan, B.A. and mahla A.G. (2013): Effect of dietary levels of spermint (mentha spicata) on broiler chick’s performance. Online J.   Anim. Feed Res. 3(4):193-196


3. Anonymous. Peppermint, In: Dombek C, ed. (1990): Lawerence Review of Natural Products. St. Louis: Facts and Comparison.


4. Ashayerizadeh,O., Dastar, B.,  Shams Shargh, M., Ashayerizadeh, A., Rahmatnejad E., and  Hossaini S.M.R. (2009): Use of garlic (Alium sativum) Black seed (Nigella sativa) and will mint (Memta longifolium) in broiler ckicken diet, J.   Anim. Vet. Advance. 8(9):1860-1863


5. Awaad, M. H. H., Abdel-Alim, G. A., Sayed, Kawkab, K. S. S., Ahmed, A., Nada, A. A., Metwalli A. S. Z. and Alkhalaf A. N. (2010): Immunostimulant Effects of Essential Oils of Peppermint and Eucalyptus in Chickens. Pakistan Vet. J. 30(2): 61-66


6. Bahraminejad, S., Asenntorfer, R.E., Riley, I.T., Zwer, P., Schultz, C.J. and Schmidt, O. (2008): Genetic variation of flavonoid defense compound concentration in oat (Avena sativa L.) entries and testing of their biological activity. Australasian Plant Breeding Conference, Christchurch, NewZealand, 18 (21):1127 - 32.


7. Bahraminejad, S., Asenstorfer, R.E. (2008): Riley IT and Schultz CJ. Analysis of the antimicrobial activity of flavonoids and saponins isolated from the shootsoats (Avena sativa L.). J. Phytopathol. 156: 1 - 7.


8. Barbour, E.K. and Danker, S. (2005): Essential oils of eucalyptus and peppermint improve the homogeneity of immune responses and performance in MG/H9N2- infected broilers. J. Am. Holistic. Vet.  Medic. Association. 24: 23-27.


9. Durrani, F.R., Sultan, A., Muhammad LatifMarri,   N., Chand, Z. (2007): Durrani Effect of Wild Mint (Mentha longifolia) Infusion on the Over All Performance of Broiler Chicks. Pakistan J. Biologic. Sci. 10 (7): 1130-1133


10. Fallah, R., Kiani, A., and Azarfar, A. (2013): Effect of Artichoke Leaves Meal and Mentha Extract (Mentha piperita) on Immune Cells And Blood Biochemical Parameters of Broilers. Glob. Vet. 10 (1): 99-102


11. Gardnier, P. (2000): Pepperminl (Mentha piperita).the longwood task force. Thecwnter for holistic pedaiatric educa tion and research, 1-16


12. Iscan, G., Kirimer, N., Kurkeuglu, M., Baser K.H. and Demirci, F. (2002): Antimicrobial screening of Mentha piperita essential oils. J. Agric. Food. Chem. 50: 3943-3946.


13. Khempaka, S., pudpila, U., and molee, W. (2013): Effect of dried peppermint (mentha cordifolia) on growth performance, nutrient digestibility, cacass traits, antioxidant peroperties, and ammonia production in broilers. Appl. Poult. Res. 22:904-912


14. Nickels, C.H.F. (1996): Antioxidants improve cattle immunity following stress. Anim. Feed. Sci. Technol. 62:59-68.


15. Schuhmacher, A., Rrichling, J. and Schnitzler, P. (2003): Virucidal effect of peppermint oil on the enveloped viruses, herpes simplex virus type 1 and type 2 in vitro. Phytomedic. 10: 504-510.


16. Taylor, D.J. (2001): Effects of antibiotics and their alternatives. Br. J. poult. Sci. 412:67-687.