Study of effect of Ginseng on testicular tissue after Orchidopexy operation in immature rat

Subject Areas : Journal of Comparative Pathobiology

Safavi, E., Mousavi, G. . ¹

1 - .

Received: 2017-02-12 Accepted : 2017-02-12 Published : 2017-06-22

Keywords: Ginseng, Testis, Cryptorchidism, Orchidopexy, Rat,

Abstract :

Perturbation of testicular descension into the scrotum result in cryptorchidism. After surgical correction of cryptorchidism (Orchidopexy), recovery of damaged testicular tissue, were observed gradually. The aim of present study, was to histological evaluation on the effect of Ginseng against testis after Orchidopexy in rat. In this study, 40 male Wistar rats (90-120 gr and 28 days old) randomly were divided in to 4 groups of 10 animals each. In first groups, after Orchidopexy, saline normal used orally for 30 days. In second groups, after Orchidopexy, Ginseng (500mg/kg/day) used orally for 30 days. In third groups, as a sham, after incision, testes were replaced from scrotum to abdominal cavity then returned to scrotum and used saline normal orally for 30 days. In fourth groups, as a healthy control, no operated. At the end of the experimental periods, testicular tissue samples were obtained for morphologic and histomorphometerical studies. The results were analyzed with one-way ANOVA and Tukey test and P 

References:

1. صفوی، س.ا.، خیاط نوری، م.،شهروز، ر. (1387): مطالعه آپوپتوز در بافت بیضه متعاقب ایجاد نهان بیضگی تجربی یکطرفه در موش صحرایی بالغ. فصلنامه علمی-پژوهشی فیض، 12(3):39-33


2. AL-Asadi, F. (2011): Effect of Vitamin C on Apoptotic Germ Cells of Cryptorchid testis in Rabbits. Bas. J. Vet. Res. 10(1):67-81.


3. Attele,  A.S., Wu, J.A., Yuan, C.S. (1999): Ginseng pharmacology: mul­tiple constituents and multiple actions. Biochemical  Pharma­cology. 58:1685-1693.


4. Babaei, H., Azari, O., Kheirandish, R., Abshenas, J. Mohammadi, N. (2010). Zinc Therapy Improves rious Effects of Experimental Unilateral Cryptorchidism: Histopathological Evaluation of Testes, Iranian Journal of Veterinary surgery, 5(12):77-88.


5. Danno, S., Itoh, K., Matsuda, T. (2000). Decreased expression of mouse Rbm3, a cold – shock Protein, in sertoli cells of cryptorchid testis. American Journal of Pathology. 156(5):1685-1692.


6. Diab, A., Abd El-Aziz, A, Hendawy, A. , Zahra, M., Hamza, R. (2012): Antioxidant role of both propolis and ginseng against neurotoxicity of chlorpyrifos and profenofos in male rats. Life Science Journal. 9(3):987-1008.


7. Gracia R., Ferrandez, L., Alvarez, F. (1990): Experimental cryptorchidism in the wistar rat. Cirugia Pediatrica. 3(3):97-102.


8. Hosseini, A.,  Zare, S., Ghaderi Pakdel, F.,  Ahmadi, A. (2010): Effects of Vitamin E and Ginseng Extract on Fertility Changes Induced by Cyclophosphamide in Rats. Journal of reproduction &infertility. 11(4):227-237.


9. Hwang, S., Sohn, S.H., Wee, J., Yang, J.B., Kyung, J.S., Kwak, Y., Kim, S.W., Kim, S. (2010): Panax ginsengimproves senile testicular function in rats. Journal of Ginseng Reserch. 34(4): 327-335.


10. Hye-Min, P., Shang-Jin, K., Hyeon-Kyu, G., Gi-Beum, S. K., Jin-Shang, K., Hyung-Sub, K. (2011): Korean red ginseng prevents ethanol-induced hepatotoxicity in isolated perfused rat liver. Korean J Vet Res. 51(2):159-164.


11. Ikeda, M., Kodama, H., Fududa, J. (1999): Role of radical oxygen species in the rat testicular germ cell apoptosis induced by heat stress. Biology of Reproduction. 61:393-399.


12. Jegou, B., Peake, R., Irby, D., Kretser, D. (1984): Effects of the induction of experimental cryptorchidism and subsequent orchidopexy on testicular function in immature rats. Biology of reproduction. 30(1):179-187.


13. Kaki, T., Sofikitis, N. (1999): Effects of unilateral cryptorchidism on controlateral sperm quality quantatity and ferfilizing capacity. Yanago Acta Medica. 42:79-86.


14. Kim ,Y. , Kim, G., Shin, J., Kim, K., Lim, J. (2010): Effect of korean red ginseng on testicular tissue injury after torsion and detorsion. Korean journal of urology. 51:794-799.


15. Kumagai, A., Kodama, H., Kumagai, J., Fukuda, J., Kawamura, K., Tanikawa, H., Sato, N., Tanaka, T. (2002): Xanthine oxidase inhibitors suppress testicular germ cell apoptosis induced by experimental cryptorchidism. Mol Hum Reprod. 8(2):118-123.


16. Liamara, R., Luis, A., Fabio, F. (2005): Sperm retrival techniques in rats with suppressed spermatogenesis by experimental cryptorchidism. Human Reproduction. 20(2):443-447.


17. Liao, B., Newmark, H., Zhou, R. (2002): Neuroprotective effects of ginseng total saponin and ginsenosides Rb1 and Rg1 on spinal cord neurons in Vitro. Experimental neurology. 173(2):224–234.


18. Meistrich, M., Wilson, G., Porter, K. (2003): Restoration of spermatogenesis in DBCP – treated rats by hormone suppression. Toxicol. SCi. 76(2):418-426.


19. Mendis, S., Kerr, J. (1990): Experimental cryptorchidism in the adult mouse: qualitative and quantitative light microscopic morphology. Journal of Andrology. 11(6):539-547.


20. Pinart, E., Sancho, S., Briz, M. (1999): Morphologic study of the testis from spontaneous unilateral and bilateral abdominal cryptorchid boars. Journal of Morphology. 239(3):225- 243.


21. Ren, L., Medan, M., Ozu, M. (2006): Effects of experimental cryptorchidism on sperm motility and testicular endocrinology in adult male rats. The Journal of Reproduction and Development. 52(2): 219- 228.


22. Saalu, L., Oluyemi, K., Omotuyi, I. (2007): a-Tocopherol (vitamin E) attenuates the testicular toxicity associated with experimental cryptorchidism in rat. African Journal of Biotechnology. 6(12):1373-1377.


23. Saleh, A.S. (2012): Effects of taurine and/or ginseng and their mixture on lipid profile and some parameters indicative of myocardial status in streptozotocin-diabetic rats. The journal of basic & applied zoology; 65:267-273.


24. Shetty, G., Wilson, G., Huhtanieme, I. (2000): Gonadotropin–Releasing Hormone analogs stimulated and testosterone ingibits the recovery of spermatogenesis in irradiated rats. Endocrinology. 141:1735-1745.


25. Shidone, T.,  Billing, H. (1994). Experimentally induced cryptorchidism increases apoptosis in rat testis. Biology of Reproduction. 51:866-872.


26. Vigodner, M., lewin, L. (2003): Evaluation of damage to the testicular cells of golden hamsters caused by experimental cryptorchidism using flow cytometry and confocal microscopy. International Journal of Andrology. 26(2): 84-90.