Molecular detection of Streptococcus uberis in cows with sub clinical mastitis by PCR method

Subject Areas : Journal of Comparative Pathobiology

Emadi, S.R., Tajik, P., Bolourchi, M., Eslampour, M.A. . ¹ (.)

Received: 2017-02-12 Accepted : 2017-02-12 Published : 2017-01-20

Keywords: Dairy cow, PCR, Streptococcus uberis,

Abstract :

With progress in monitoring and controlling of contagious mastitis and with development of dairy farms the importance of environmental mastitis such as Streptococcus uberis has been increased. This study conducted to evaluate frequency of Streptococcus uberis in cows suffering from subclinical mastitis. Study was performed in 4 dairy farms in Tehran province. Samples were collected from cows with subclinical mastitis which diagnosed with CMT, and were sent for bacterial culture, DNA preparation with commercial kit and PCR assay. Final product was electrophoresed in 2 percent agarose gel with 80 V during 2 hours. A total of 64 of 85 samples had amplicons with a size of 445 bp. A total of 6.6% of cows were suffering from subclinical mastitis with Streptococcus uberis pathogen. In conclusion monitoring and control methods of mastitis with environmental agents such as Streptococcus uberis Should be more considered and also could use more accurate and faster molecular methods to differentiate it.