Histopathological evaluation of cyclosporin gel on full thickness skin transplantation (Allograft) in the rat

Subject Areas : Journal of Comparative Pathobiology

Mofidi, A., Jahandideh, A., Hesaraki, S. . ¹

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Received: 2016-08-25 Accepted : 2016-08-25 Published : 2016-07-22

Keywords: Cyclosporine gel, Full thickness allograft trans, Rat,

Abstract :

Nowadays, it is used to skin graft in spread. Cyclosporine is one of the most use and effective immunosuppressive drugs that it has side effect and toxicities on CNS, kidney and liver. In this research is tried to investigation of 0.5% cyclosporine gel effectiveness on allograft full thickness skin rejection in rat. In this experimental study is used to 45 wistar male adult rats as allograft recipient and 15 spiral hole (SD) male adult rats as allograft donor with age range between 14 to 15 week and mean weight: 200±10 gr. After general anesthesia, circles with 1.5 cm diameter containing epidermal and dermal layers had separated from SD donor rats with scalpel cutting and then these parts were grafted to shoulder center (midline) of wistar recipient rats. After transplantation, rats randomly divided to 3 groups with 15 rat (3groups of 5 each) (control, test and 0.5% cyclosporine gel) and then each of them was divided to 3 group with 5 rat (sampling groups in days: 3, 7 and 15). Any treatment is applied in control group. 10 mg/kg IP cyclosporine injected to test group, and in third group, 0.5% cyclosporine gel was applied in the graft place, daily. Then on days: 3, 7 and 15 after graft, it was done sampling from the graft location and applied histopathological studies on it. Then, Statistics data analyzed using kruskal wallis test with SPSS software. Investigations confirmed that cyclosporine gel using caused to significant reduce of vasculitis, folliculitis, dermatitis and dermal degeneration on 3 and 7 days after graft in comparison to control group (P<0.05) and single use of cyclosporine gel on day 15 after graft did not cause to skin graft rejection. By attention to this test challenges, cyclosporine gel has significant effects on Acceptance of skin grafts, but its single use did not cause to skin graft rejection. To more effect on skin graft acceptance can use to cyclosporine gel in combination with other immunosuppressive drugs.