Comparative study of sedation and pre-anesthetic effects of polar, semi polar and non-polar fractions of vinca minor extract in comparison with midazolam in rat

Subject Areas : Journal of Comparative Pathobiology

A. Rezaei, ¹ , E. Issabeagloo, ² , مهرداد Pashazadeh, . ³

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Received: 2013-10-23 Accepted : 2014-02-24 Published : 2015-01-21

Keywords: Sedation, Pre-anesthetic, Polar, Semi-polar, Non-polar, Vinca Minor,

Abstract :

Periwinkle is a plant known as a Vinca minor in Persian. This plant contain bitter substancecalled Vincine, Tannin, Pectin, Carotene, vincamine Alkaloids, Vinci nine, Catarin, and a kindof Saponin and large amount of Vinine Alkaloids and Pubcine. All parts of this plant can beused; especially the leaves that can be picked in any season and seared by heat. The purpose ofthis study is evaluating sedation and pre-anesthetic effects of polar fractions, semi polar andnon-polar of Vinca minor plant in comparison with midazolam found in rats. 60 Wistar rat headin different groups is applied in these tests. Three polar, semi-polar, and non-polar fractions and0.04 mg/kg dose of midazolam and dimethyl sulfoxide (DMSO) with equal volume aretransfused into peritoneum as a drug, half an hour before experiment. In a test the sedation andpre-anesthetic induction time and sleeping time effects are surveyed by seconds and recorded bychronometer. Findings show that in medical team the mentioned doses of extract meaningfulboth in infused dream by ketamine and increase in life time (p<0.01). According to sedationand pre-anesthetic effects of this plant, studies indicate that polar fraction of Vinca minor with100 mg/kg dose has sedation, pre-anesthetic effects.