Evaluation of the effect of different doses of folic acid supplementation on serum lipid profiles in experimental obstructive cholestasis in rat

Subject Areas : Journal of Comparative Pathobiology

Mohammadian, Z. *, Eidi, A. , Mortazavi, P. , Tavangar, SM. , Asghari, A. . ¹ ( )

Received: 2014-05-30 Accepted : 2014-09-19 Published : 2014-09-23

Keywords: Bile duct ligation, Cholesterol, Folic Acid, Lipoprotein,

Abstract :

Hypercholesterolemia was the result of the cholestasis and failure of cholesterol and bile saltsclearance through the bile duct.The main proposal of this study is to investigate the effect offolic acid supplementation on serum lipids composition in a rat model of cholestasis. 81 maleWistar rats were randomly divided into nine experimental groups with 9 rats in eachgroup.Group 1, normal control rats ,Group 2, sham-operated rats (The rats in this groupunderwent the same procedure except that the bile duct ligation) ,Group 3, cholestatic rats,Groups 4-6, folic acid control rats;Groups 7-9, cholestatic rats treated with folic acid; Folic acidtreated groups were given folic acid (1,5,10 mg/kg) respectively , once a day by gavage for 28days. Cholestasis was produced by Bile Duct Ligation (BDL). Serum samples were analyzedbiochemically and lipids profile were measured according to standard protocols. cholestatic ratsgroup showed higher concentrations of total cholesterol (TC) , triglyceride (TG) and low densitylipoprotein cholesterol (LDL) in serum. Administration of folic acid supplementation ,significantly decreased the serum levels of TC , TG, and LDL and increased the serum level ofhigh-density lipoprotein cholesterol (HDL). Cholestasis induced hypercholesterolemia and Folicacid supplementation showed hypolipidemic and hypocholesterolemic effects in cholestatic rats.These effects on the lipid profile varied with the different concentrations of folic acid. Ourresult suggested that Folic acid can be used an antilipidemic agent in pation with cholestasis.