The evaluation of antibacterial activity of TiO2 nanoparticles , EDTA and garlic on standard strain of Pseudomonas aeroginusa by microdilution method

Subject Areas : Journal of Comparative Pathobiology

مینا سعادت ¹ , شهلا رودبارمحمدی ² , رمضانعلی خاوری نژاد ³ , الهام تقوی ⁴

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Received: 2010-10-14 Accepted : 2011-01-04 Published : 2011-08-23

Keywords: Antibacterial agent, Pseudomonas aeroginusa, TiO2 nanoparticles,

Abstract :

Importance of microbial contamination in food industrial is always important health problemespecially in high risk population such as children and old individual. TiO2 nanoparticles wereobtained through the hydrolysis of TiCl4 (Titanium tetrachloride).It was slowly added intodistilled water under constant rotation for 5 hours. This Solution was dried at the 80-100 0C andcalcinated at the 5500C. Size and type of these Nanoparticles were characterized by scanningelectron microscope (SEM) and X-Ray-: Diffraction (XRD) pseudomonas aeroginusa strain(ATCC 27853) were cultured on nutrient agar medium (NA) for 24h at 370C. Suspension byconcentration 1×106 cells/ml was prepared. Minimum Inhibitory Concentration (MIC) test forTiO2 and EDTA and garlic were evaluated.Evaluation of Morphology and diameter of the TiO2 nanoparticles with SEM showed thatnanoparticles were spherical with diameter between 40-65nm. MIC of TiO2 and EDTA andgarlic on pseudomonas aeroginusa ATCC27853 were 2/2, 24/92 and 40 μg/ml respectively.In this study we synthesized the TiO2 nanoparticles with chemical method and showed that ithas antimicrobial activity compared with garlic, EDTA and positive control, so it couldeliminate Pseudomonas aeroginusa in field of food industrial specially in packaging.