Study of helicobacter Spp. infection in stray dog's stomach in Mashhad

Subject Areas : Journal of Comparative Pathobiology

فرنوش ارفعی ¹ , شهرام جمشیدی ² , معصومه عظیمی ³ , حسین دبیری ⁴ , محمدرضا زالی ⁵

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Received: 2011-02-04 Accepted : 2011-06-02 Published : 2011-08-23

Keywords: Helicobacter, Stray dogs, Stomach, Mashhad,

Abstract :

The present study was performed to determine if Helicobacter spp is present in the stomach ofstray dogs by different diagnostic methods in mashhad.48 stray dogs were studied. The presence of Helicobacter spp was determined by single PCR,Urea test, cytology and culture from fundus, body and gastric juice. Genus specific PCRpositive samples were further evaluated for H.pylori, H.felis and H.heilmannii using specificprimer pairs. Helicobacter spp DNA was detected by single PCR in 40(83.3%) fondues,40(83.3%) body and 46(95.8%) gastric juice samples. H.pylori specific-species PCR wasuniformly negative. The prevalence of H.felis was 64.58% and H.heilmannii was 89.6% in thestomach. High helicobacter spp. Infection rate in dog's stomach and possibility of zoonotictransmission to human, proper hygienic control is necessary to keep the risk to a minimum.