Evaluation of the biofilm-forming ability of Staphylococcus aureus isolates from bovine mastitis in Iran
Subject Areas : Journal of Comparative Pathobiologyبابک Khoramian 1 , M.E Emaneini 2 , محمود Bolourchi 3 , M.A Eslampour 4 , امیر Niasari-Naslaji 5 , مرضیه Aligholi 6 , عباس Barin 7 , سعید Sattari 8 , پرویز Hovareshti 9
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Keywords: Biofilm, Staphylococcus aureus, Mastitis, Cattle,
Abstract :
One important virulence trait utilized by Staphylococcus aureus is the ability to form biofilms ,this organism is able to adhere to and penetrate inside bovine mammary epithelial cells. In S.aureus polysaccharide intercellular adhesin (PIA) was encoded by icaA and icaD genes.Production of PIA is currently responsible for staphylococcal biofilm development. BiofilmInfections are generally chronic and difficult to treat. The aim of this study was to determine theprevalence of phenotypic and genotypic of biofilms from bovine mastitis infections in Iran. Atotal of 90 samples of Staphylococcus aureus were collected from raw milks of five dairy farmsnear Tehran. All of the samples were diagnosed according to the National mastitis council(NMC) methods. Staph aureus isolates confirmed by a polymerase chain reaction (PCR)-basedmethod. In order to determine the biofilm formation capacity of the studied S. aureus strains,microtiter plate assay was performed. All the strains were screened for the presence of ica locusand icaA and icaD genes by PCR. The results of this study showed that 79 of 90 (87.7%) strainsof S.aureus were found to possess the genes for biofilm production as evidenced by positiveamplification of the ica locus as well as the icaA and icaD genes. The analysis of phenotypic bymicrotiter plate method showed that 4.4%, 40%, 43.3% of the S. aureus isolates producedstrong, moderate or weak biofilm respectively, and 12.2% unable to produce biofilm.