Isolation and screening of pectinase producing Aspergillus niger and use of whey to optimize enzyme production
Mahsa pouya
1
(
1M.Sc of Industrial Microbiology, Department of Microbiology, Faculty of New Science, Tehran Medical sciences, Islamic Azad university, Tehran, Iran
)
Shokoofeh Ghazi
2
(
Department of Microbiology, Faculty of Basic Science, Tehran Medical Branch, Islamic Azad University, Tehran, Iran
)
امیر توکمه چی
3
(
گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه
)
Keywords: Aspergillus niger, Pectinase enzyme, DNS method, Whey,
Abstract :
Abstract Aims and Background: Pectinase that can break down pectin in plant cell walls is one of the most important industrial enzymes in the world, which can be isolated from a wide range of microorganisms such as bacteria and fungi. Therefore, considering the importance and applications of this enzyme in food processing, agriculture, the purpose of this research is isolating and screening pectinase-producing fungi from some of rotten fruits and vegetables, as well as optimizing the enzyme production condition. Materials and methods: Isolation and identification of pectinase producing fungi from rotten fruits (peach, plum and apple) and vegetables (onion) were done. Screening the most capable fungal strains producing pectinase was used by cultivation on specific Pectin Agar medium, staining with lactophenol cotton blue and slide culture method. To identify the isolated fungi more accurately, the molecular technique such as 18S rRNA sequencing was used. Optimizing the production rate of pectinase enzyme carried out using pectin substrate accompanied by different Lactic Whey and Permeate whey under different temperatures and pH levels, and the activity of enzyme was measured by the standard DNS method. Results: one Aspergillus niger fungus producing pectinase enzyme was isolated from onion. Results for optimization conditions shows that the identified strain, shows an enzyme activity equal to 103/018 (IU/ml) in the presence of permeate whey and pectin substrate, under incubation at 25 C° ,140 rpm, and pH 6.5. While, pectinase production with only pure pectin substrate was lower equal to 92.717 (IU/ml) when incubation at 25 C° , same rpm and pH 7. Conclusions: Potent native fungal strains isolated from our country are suitable candidates for the production of important industrial enzymes such as Pectinase. Also using cost effective substrates like Whey can be replaced by expensive pure substrates in production industry for the important enzymes production process.
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