New methods for molecular assessment and surveillance of Enteroviruses as viral indicators in aquatic environments
Subject Areas : Virology
Reza Sedighi Shiri
1
,
Mohammad Kargar
2
,
Farshid Kafilzadeh
3
,
Niloofar Neisi
4
1 -
2 - Department of Microbiology, Zand Institute of Higher Education, Shiraz, Iran
3 - دانشگاه آزاد اسلامی، واحد جهرم، گروه میکروبیولوژی
4 - Infectious and Tropical Diseases Research Center, Health Research Institute, Department of Medical Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Keywords: Waterborne viruses. Enteroviruses. Virus indicators. Biomonitoring. Concentration methods.,
Abstract :
Environmental water quality monitoring is a global issue, and many efforts are being made to manage it properly. Enteroviruses are one of the most significant waterborne viruses. These viruses pose significant risks to the public health of humans, animals, and aquatic species through the discharge of sewage, agricultural operations, and runoff from landfills, penetrating into surface and groundwater. Early, rapid, and effective detection of culturable EVs in aquatic systems is essential to ensure water hygiene levels and implement appropriate water and wastewater treatment strategies. Biological monitoring of aquatic environments, despite the lower cost of measuring bacterial indicators such as fecal coliforms, Enterococci, and Clostridium perfringens, does not guarantee the absence of enteric viruses due to their resistance to chlorination and tertiary treatment. Enteroviruses are one of the viral indicators for monitoring of water quality and wastewater treatment systems, which have not yet been widely and commonly used. In this article, the practical adequacy of various new methods for the concentration and molecular identification of Enteroviruses in water and wastewater is assessed by evaluating reputable scientific databases.
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