The Synergistic Effect of Aerobic Exercise and Garlic and Stevia Extract on Sema3e Gene Expression and Body Weight in Obese Male Wistar Rats
Subject Areas : New studies in exercise metabolism and physical activityparastoo roshandel 1 , reza Rezaei 2 , Sayyed-Javad Ziaolhagh 3 , Neda Aghaei bahman begloo 4
1 - Doctoral student of the Department of Physical Education and Sports Sciences, Aliabad Katul Branch, Islamic Azad University, Aliabad Katul, Iran.
2 - 2. Assistant Professor, Department of Physical Education and Sports Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
3 -
4 - 2. Assistant Professor, Department of Physical Education and Sports Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
Keywords: Semaphorin 3e, Garlic extract, Stevia extract, Aerobic exercise, Childhood obesity,
Abstract :
Background and Aim: The expression of the Sema3e gene plays a crucial role in the regulation of adipocyte growth and development and may be involved in obesity- and metabolism-related processes. This study aimed to investigate the effect of obesity induction on Sema3e gene expression along with garlic and stevia supplementation and aerobic exercise in male Wistar rats.
Materials and Methods: In this experimental study, 35 male Wistar rats were subjected to a high-fat diet for 12 weeks to induce obesity. Finally, 30 obese rats were identified using the Lee index and randomly divided into five groups: control, obese, obese+garlic, obese+aerobic exercise, and obese+garlic+exercise. Aerobic training consisted of running on a treadmill for 30 minutes per day at 8 m/min, 5 days per week, for 12 weeks. Garlic and stevia extract (250 mg/kg body weight) were added to the daily drinking water. At the end of the interventions, hypothalamic tissue was collected, and relative fold-change of Sema3e expression was measured using real-time PCR. One-way ANOVA and Tukey’s post-hoc test were used to assess differences between groups at a significance level of 5%.
Results: A significant difference was observed in Sema3e gene expression between groups (p = 0.011). Tukey’s test revealed that the obese+garlic and obese+stevia groups had significantly lower Sema3e expression compared to the obese+exercise group (p = 0.009 and p = 0.024, respectively).
Discussion and Conclusion: The results demonstrated that obesity alters Sema3e gene expression, and garlic and stevia supplementation can modulate this alteration. Moreover, their combination with aerobic exercise showed a distinct effect, suggesting a practical approach for obesity management.
1. Alex S, Boss A, Heerschap A, Kersten S. Exercise training improves liver steatosis in mice. Nutrition & metabolism. 2015;12(1):29.
2. Lu Q, Zhu L. The role of semaphorins in metabolic disorders. International Journal of Molecular Sciences. 2020;21(16):5641.
3. Nam JS, Ahn CW, Park HJ, Kim YS. Semaphorin 3 C is a novel adipokine representing exercise-induced improvements of metabolism in metabolically healthy obese young males. Scientific reports. 2020;10(1):10005.
4. Shimizu I, Yoshida Y, Moriya J, Nojima A, Uemura A, Kobayashi Y, Minamino T. Semaphorin3E-induced inflammation contributes to insulin resistance in dietary obesity. Cell metabolism. 2013;18(4):491-504.
5. Bordenave S, Metz L, Flavier S, Lambert K, Ghanassia E, Dupuy A-M, et al. Training-induced improvement in lipid oxidation in type 2 diabetes mellitus is related to alterations in muscle mitochondrial activity. Effect of endurance training in type 2 diabetes. Diabetes & metabolism. 2008;34(2):162-8.
6. Horuzsko D. Allicin reverses diabetes-induced dysfunction of human coronary artery endothelial cells. 2019.
7. Shakiba E, Sheikholeslami-Vatani D, Rostamzadeh N, Karim H. The type of training program affects appetite-regulating hormones and body weight in overweight sedentary men. Applied Physiology, Nutrition, and Metabolism. 2019;44(3):282-7.
8. Nam JS, Ahn CW, Park HJ, Kim YS. Semaphorin 3 C is a novel adipokine representing exercise-induced improvements of metabolism in metabolically healthy obese young males. Scientific reports. 2020;10(1):1-10.
9. Mirzaei-Aghsaghali A. Importance of medical herbs in animal feeding: A review. Ann Biol Res. 2012;3(9):3-933.
10. Neufer PD, Bamman MM, Muoio DM, Bouchard C, Cooper DM, Goodpaster BH, et al. Understanding the cellular and molecular mechanisms of physical activity-induced health benefits. Cell metabolism. 2015;22(1):4-11.
11. Egan B, Zierath JR. Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell metabolism. 2013;17(2):162-84.
12. Peteliuk V, Rybchuk L, Bayliak M, Storey KB, Lushchak O. Natural sweetener Stevia rebaudiana: Functionalities, health benefits and potential risks. EXCLI journal. 2021;20:1412.
13. Abdallah SH, Mostafa NM, Mohamed MAEH, Nada AS, Singab ANB. UPLC-ESI-MS/MS profiling and hepatoprotective activities of Stevia leaves extract, butanol fraction and stevioside against radiation-induced toxicity in rats. Natural Product Research. 2021:1-7.
14. Giles ED, Jackman MR, MacLean PS. Modeling diet-induced obesity with obesity-prone rats: implications for studies in females. Frontiers in Nutrition. 2016;3:50.
15. Soares TS, Andreolla AP, Miranda CA, Klöppel E, Rodrigues LS, Moraes-Souza RQ, et al. Effect of the induction of transgenerational obesity on maternal-fetal parameters. Systems biology in reproductive medicine. 2018;64(1):51-9.
16. Eidi A, Eidi M, Oryan S, Esmaeili A. Effect of garlic (Allium sativum) extract on levels of urea and uric acid in normal and streptozotocin-diabetic rats. Iranian Journal of Pharmaceutical Research. 2004;3(2):52-.
17. Ajagannanavar SL, Shamarao S, Battur H, Tikare S, Al-Kheraif AA, Al Sayed MSAE. Effect of aqueous and alcoholic Stevia (Stevia rebaudiana) extracts against Streptococcus mutans and Lactobacillus acidophilus in comparison to chlorhexidine: An in vitro study. Journal of International Society of Preventive & Community Dentistry. 2014;4(Suppl 2):S116.
18. Inoue DS, Bin Maideen MF, Jiménez-Maldonado A, Lira FS. Role of Neuronal Guidance Cues in the Pathophysiology of Obesity: A Peripheral and Central Overview. Current Pharmaceutical Design. 2021;27(21):2512-21.
19. Shapoori S, Mosayebi G, Ebrahimi Monfared M, Ghazavi A, Khansarinejad B, Farahani I, Ganji A. Gene expression of semaphorin-3A, semaphorin-7A, neuropilin-1, plexin-C1, and β1 integrin in treated-multiple sclerosis patients. Neurological Research. 2020;42(9):783-8.
20. Koda T, Namba A, Kinoshita M, Nakatsuji Y, Sugimoto T, Sakakibara K, et al. Sema4A is implicated in the acceleration of Th17 cell-mediated neuroinflammation in the effector phase. Journal of Neuroinflammation. 2020;17(1):1-9.
21. Sezgin G, Kar F, Uslu S. The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients. Archives of physiology and biochemistry. 2022;128(2):558-67.
22. He J, Zhu N-L, Kong J, Peng P, Li L-F, Wei X-L, et al. A newly discovered phenylethanoid glycoside from Stevia rebaudiana Bertoni affects insulin secretion in rat INS-1 islet β cells. Molecules. 2019;24(22):4178.
23. Salehi B, López MD, Martínez‐López S, Victoriano M, Sharifi‐Rad J, Martorell M, et al. Stevia rebaudiana Bertoni bioactive effects: From in vivo to clinical trials towards future therapeutic approaches. Phytotherapy Research. 2019;33(11):2904-17.
24. Lemus-Mondaca R, Vega-Gálvez A, Rojas P, Stucken K, Delporte C, Valenzuela-Barra G, et al. Antioxidant, antimicrobial and anti-inflammatory potential of Stevia rebaudiana leaves: effect of different drying methods. Journal of Applied Research on Medicinal and Aromatic Plants. 2018;11:37-46.
