Investigating the antimicrobial and biofilm inhibitory effects of gingerol against multidrug resistant isolates of Pseudomonas aeruginosa
Subject Areas : Biotechnological Journal of Environmental MicrobiologyMarzieh Aghaie 1 , Leila Asadpour 2 , Masoud Mokhtary 3 , Hadi Habibollahi 4
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Keywords: Gingerol, Antimicrobial, anti-Biofilm, Pseudomonas aeruginosa,
Abstract :
Pseudomonas aeruginosa is a Gram-negative bacterium and one of the most important opportunistic pathogens causing hospital infections that usually acquires multidrug resistance. This study was conducted to investigate the effect of gingerol in inhibiting the growth and biofilm formation of Pseudomonas aeruginosa isolates.
This study was conducted on P. aeruginosa isolates with multiple antibiotic resistance. The disk diffusion method was used to determine the antibiotic resistance of the strains. The diffusion and broth microdilution methods were used to investigate the antimicrobial effect of gingerol. The anti-biofilm effect of gingerol was investigated by the microplate method, and its effect on the expression of the biofilm pslA gene by real-time PCR.
Gingerol showed an antimicrobial effect against pathogenic P. aeruginosa strains, and its minimum inhibitory concentration (MIC) in varied between 512-1024 µg/mL. The use of this substance caused a 44-52% reduction in biofilm formation of isolates (P˂0.05). Gingerol also significantly reduced (more than 52%) the expression of the pslA gene.
The present results indicate the possibility of using gingerol to combat planktonic and biofilm forms of antibiotic-resistant bacteria. The use of this substance with or instead of antibiotics may be a way to reduce the use of existing antibiotic drugs and thus reduce the side effects and treatment costs of infectious diseases.
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